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Take SYBR Green I as the intercalating fluorescence dye in Real-time PCR,the overexpression of WIPK and SIPK,which were the key genes of MAPK cascades,and the resistant gene PR1 in tobacco treated with copper and magnesium solution were studied.

采用SYBR GreenⅠ作为荧光染料的Real-time PCR方法，明确了铜、镁素能调控烟株体内蛋白激酶级联信号转导途径的关键基因WIPK、SIPK及抗病信号通路末端PR1基因的过量表达，进一步揭示了矿质营养调控烟草对病毒抗病性的分子机制。

Earlier on Sunday, as crisis talks were held in Berlin, the Finance Minister Peer Steinbrueck launched a stinging attack on the managers of Hypo Real Estate, accusing them of misinforming the government by failing to review the true scale of the lender's problems.

早前，当危机会谈星期日在柏林举行时，财政部长史坦布律克对Hypo Real Estate 的经理们发起了激烈的攻击，指责他们因为未能认清贷款问题的真正规模而误导政府。

Bifidobacteria in feces samples can be directly quantitated or semiquantitated by real-time PCR method or common PCR method.

利用PCR、real-time PCR直接半定量和定量粪便中的双歧杆菌可行。

Real Food upends the conventional wisdom on diet and health and explains our taste for good things.

Real Food颠覆了传统上对健康食物的观念。

Therefore it is interesting to clone genes related to lemma and palea. In this thesis, six genes from the lemma/palea related gene pool which is established by Liu (2003) were further studied. Those genes are salT gene (salt-induced protein), GA-SPY gene (gibberellin action negative regulator SPY-related protein), U2AF gene (U2 snRNP auxiliary factor, small subunit-related protein), kinesin-like gene, DnaJ-like gene, and EF hand gene. At first, the gene expression in leaves and 1~4 cm inflorescences of normal lemma/palea, smaller lemma/palea, and stunted lemma/palea was compared by Real Time RT-PCR.

从刘(2003)所建立水稻颖花发育相关候选基因库中，挑选6个基因：salT基因(salt-induced protein)、GA-SPY基因(gibberellin action negative regulator SPY-related protein)、U2AF基因(U2 snRNP auxiliary factor，small subunit-related protein)、kinesin-like基因、DnaJ-like基因、及EF hand基因，以水稻正常型颖花、小颖花突变体及内外颖退化突变体之叶片及1~4公分幼花序为材料，利用Real-Time RT-PCR分析，发现salT基因在突变体幼花序中大量表现。

The Christmas theme of Pacific Place is Real Christmas Spirit this year. The historic Christmas bells are all over the place and around the corners. Along with a huge and gorgeous Christmas bell, they chime to remind you that Christmas is coming. Several Christmas events will be held to celebrate the festival and share the joyful moment with all shoppers.

今年圣诞太古广场以&Real Christmas Spirit&为主题，历史悠久的圣诞钟遍布商场每个角落及藉著华丽大型圣诞钟的清脆钟声向您预布佳节到临，由此亦幻化出一连串精彩节日表演，与购物人士一同体验普世欢腾的圣诞气氛。

Their recent release "Something Real" is currently available in record shops in China, and has been getting considerable airplay on the Australian Broadcasting Company airwaves.

他们新出的唱片&Something Real&正在中国各唱片店热卖，并在澳大利亚广播公司的电台热播。

I didn't even get a chance to check out THE REAL Barneys New York (if you know me by now, you understand my disappointment J ).

我甚至是找不到机会去 THE REAL Barneys New York （如果你了解我，你就会知道我现在有多失望）。

Giemsa staining was used to observe the growth of C.andersoni,and transmission electron microscope was used to observe the developmental stages of C.andersoni.MTT assay was used to evaluate the toxicity of nitazoxanid and ginkgoic acids to HCT-8 cells.C.andersoni parasites growing in HCT-8 cells were treated with different concentrations of NTZ and GAs,and the copies of COWP gene were determined by Real-time PCR.

安氏隐孢子虫卵囊感染人结肠癌(HCT-8)细胞，Real-time PCR技术测定RPMI-1640培养液中分别加入不同浓度胎牛血清(fetal calf serum，FCS)、葡萄糖(glucose，GLU)、维生素C(ascorbic acid，ASC)、叶酸(folic acid，FOLIC)、泛酸钙(calcium pantothenate，CAPAN)和胰岛素(insulin，INSULIN)后虫体的增殖效果。

Real Food reveals why traditional foods are actually healthy: not only egg yolks, but also cream, butter, grass-fed beef, wild salmon, roast chicken skin, and more.

Real Food告诉我们为什么传统的食物是健康的：不仅仅只有蛋黄，还有乳酪，黄油，牛肉，野生的大麻哈鱼，烤鸡。。。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐