查询词典 reagents

To further testify the high efficiency of these new reagents in the synthesis of sterically hindered peptides, we successfully synthesized the extensively -N-methylated peptide moiety of the antineoplastic and lipophilic depsipeptide Dolastatin 15 using reagent BEP and FEP. We also synthesized the highly hindered tetrapeptide segment of Cyclosporin A using reagent BEMT and BEP, respectively.

为进一步检验这类吡啶正离子型缩合剂在有空间位阻的多肽合成中的性能，我们以BEP和FEP为缩合剂，成功地合成了具有抗肿瘤活性的亲脂性酯肽Dolastatin 15分子中高N-烷基化的肽链部分；分别以BEMT和BEP为缩合剂合成了生物活性肽Cyclosporin A中空间位阻最大的四肽片断。

Methods Mix appropriate of the negative and positive in the box by looking at serum, compare the differences between different reagents and CO values of differents titers, screen out the suitable blending ratios, then subpackage、refrigerate、preserve to use.

利用试剂盒内的阴、阳性对照血清按一定量混合，通过比较不同试剂、不同滴度的CO值，筛选出合适混合比例，分装冷冻保存使用。

Learn the significance of sulphonation, various sulphonation reagents and active sequence.

了解磺化反应的重要性，各种磺化试剂及其活性顺序。

Low valent selenium and telluronium compounds have high reducing selectivity to be used to form carbon-hydrogen bonds as special reducing reagents.

低价硒、碲化合物具有良好的还原选择性，可用于多种碳-氢键的形成，碲叶立德和α-芳硒基取代的胂叶立德均可发生Wittig型反应，是形成碳-碳双键的重要方法。

Low valent selenium and telluronium compounds have high reducing selectivity to be used to form carbon-hydrogen bonds as special reducing reagents.

低价硒、碲化合物具有良好的还原选择性，可用於多种碳-氢键的形成，碲叶立德和α-芳硒基取代的胂叶立德均可发生Wittig型反应，是形成碳-碳双键的重要方法。

We provided biomimetic synthetic methods for tetrahydro-β-carboline by using the benzimidazoline as a new tetrahydrofolate coenzyme model with tryptamine as nucleophile reagents under the condition of acid.

由四氢-β-咔啉类化合物，可制得多种具有较好抗病毒、抗菌和抗肿瘤活性的β-咔啉类生物碱，因而四氢-β-咔啉类化合物的合成受到人们的广泛关注。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例，分成三份，分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本，用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA，以RT-PCR方法扩增β-Actin基因，经1.5%琼脂糖凝胶电泳，溴化乙锭染色，紫外透射仪下观察分析。

Write equations for all steps in the synthesis of treelike compound from 1,3,5-trimethylbenzene using any other needed reagents.

答：用1，3，5-三甲基苯及其它试剂为原料合成树状物

Dithioester and trithiocarbonate RAFT reagents were synthesizedby different methods, and were used for the polymerization of styrene ormethacrylates, by thermal, thermo-initiator and photo-initiation.

针对这一问题，本文尝试用三种方法合成了结构不同的RAFT试剂，考察了热引发、引发剂引发以及紫外光引发条件下，二硫酯／三硫酯调控的常见单体苯乙烯以及丙烯酸酯的RAFT聚合。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。