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All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Dron had never once been drunk or ill since he had been appointed elder; he had never after sleepless nights or severe labour shown the slightest signs of fatigue; and though he could not read or write, he never forgot an account of the pounds of flour in the huge waggon-loads he sold, and of the money paid for them, nor missed a sheaf of wheat on an acre of the Bogutcharovo fields.

德龙在全部任职期间没有醉过一次酒，没有生过一次病；不论是一连几天几夜不睡觉，也不论干了多劳累的话，从来没有露出过一丝倦容，他虽然目不识丁，却从来不曾忘记一笔帐，他轻手卖掉无数车的面粉，从来也没有忘掉——普特，他从来没有忘掉在博古恰罗沃的每俄亩土地上收获的任何一堆粮食。

In the test, we use CCD camera to obtain the videos of the well-cutting picks and the worn-cutting picks, then use Matlab to read image and process it, and then use the method of statistics to calculate the average area of the two cutting picks, at last can get the wear rate of cutting picks.

用CCD摄像机获得完好截齿和磨损截齿的视频段，然后用Matlab读取图像并对其处理，利用统计学的方法，快速计算出两个截齿的平均面积，从而得到截齿的磨损率。

Of all ages, people who read this chapter of Tao Te Ching, most of them explained that when world is peaceful and there is no war, steed and swift horse becomes useless, then, they are used to furrow field and carry fertilizer cart.

自古以来，读道德经的人对此章的解释都是说，当天下太平，没有战争时，那些千里马，善走的好马都变得英雄无用武之地了，再好的千里马，也只能利用来耕田、拉粪车而已。

Most of us probably read Margaret Mitchell's "gone with the wind", and are impressed by Scarlett , the heroine of the novel, who brings herself and the family through the bitter years of the Civil War and regains her footing in Atlanta.

也许很多人都读过米切尔的《飘》而且对于女主角Scarlett记忆犹新。她带着自己和家庭渡过了艰辛的内战并且之亚特兰大重新立足。

The promise of a Virgin-born son as a sign of the firmness of God's covenant of old with David and his house; the now unfolded meaning of the former symbolic name Immanuel; even the unbelief of Ahaz, with its counterpart in the questioning of Joseph,'all this' could now be clearly read in the light of the breaking day.

的承诺处女出生的儿子，作为一个标志的坚定性上帝的盟约旧与戴维和他的房子；现在开展的意义，前符号名称伊曼纽尔；甚至unbelief的亚哈斯，与它对应的，在质疑约瑟夫，'这一切'，现在可以清楚地阅读，根据打破天。

Austrian law is likely to be staggered on the opposite time of the outbreak, at the same time must not be exposed to their own 3 FS before the foundation keep silent in the CD, when read carefully, the faster the first time a big fireball will be to come out, and then run around the pillars to the distance, after all, are the opposite of the skills of flight time, one is able to escape for a while, there can be up and down the terrain map is the best, courage would be more dangerous, but there are Two-base, but also about you, probably is this way, RP is not the risk of poor or less basic.

我还见过3奥法，贼法法，法法骑，贼法骑，这些里面最惧怕的还是贼法骑吧。3奥法很容易就可以错开对面的爆发时间，一定不要把自己同时暴露在3个FS面前，留着根基CD在被沉默的时候，谨慎读条，迅捷在中第一个大火球的时候就要给出来，然后围绕柱子向远方跑，毕竟对面的技能都是有飞行时间的，能躲开一会就是一会，有能上上下下地形的地图是最好的，勇气会比较危险，不过有双根基，也差不多吧，大概就是这个样子，RP不差的话基本危险还是不大的。

Although market whole growth puts delay, but grow quickly as user of domestic mobile phone, add cost of mobile phone endowment to reduce and of big screen, smartphone gain ground further, domestic mobile phone read a number to appear to grow apparently, what occupy scale to already arrived from the 2.7% growth 2006 5.9% 2007, and China movesThose who move moveBusiness of the town that use a book was obtained last year grow substantially.

尽管市场整体增长放缓，但随着国内手机用户迅速增长，加上手机资费下调以及大屏幕、智能手机的进一步普及，国内手机阅读人数出现了明显增长，所占比例已从2006年的2.7%增长到2007年的5.9%，而中国移

Wenzhou pens Association Secretary-General Zhang Shao-Qing read out a letter of congratulation, ZHANG Han-ping, chairman of the Association of Wenzhou pens speech congratulated the dealer made a statement, the Chinese Association of high-chairman of the pens was made a warm speech, the text is Pen made the rapid development of express warmest congratulations.

温州市制笔协会秘书长张少清宣读了贺信，张汉平，对温州制笔协会会长表示祝贺讲话的经销商发了言，对高笔华人协会主席发了热情洋溢的讲话，文本笔使得表示最热烈的祝贺快速发展。

In 1815 he read it to the London Horticultural Society and instructed its members to 'make the surface of your green house roof parallel to the vaulted surface of the heavens, ot to the plane of the sun's orbit'.

在1815年他读到它以伦敦园艺学会，并指示其成员'，使表面上你的绿房子的屋顶平行向拱形表面的老天爷，职能治疗，以平面太阳的轨道。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。