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Methods:All of 40 male Sprague-Dawley rats were divided into 3 groups:control group(group A, n = 10), diabetic group for 4 weeks(group B, n = 14), and diabetic group for 12 weeks(group C, n = 16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NF-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组:A组为正常对照组(10只),B组为糖尿病4周组(14只),C组为糖尿病12周组(16只),采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后,A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能,并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达,同时采用电泳迁移率变动分析法进行NF-κB 活性检测。

The rats demonstrating an average of at least 280 complete contralateral turns/ 40 min for 4 times in response to apomorphine were selected as PD ra...

结果:发现6-OHDA注入大鼠右侧中脑黑质后,大鼠向损毁对侧的旋转次数明显增加(大于280圈/40min),PD模型成功率为50.5%。

Methods All of 40 male Sprague-Dawley rats were divided into 3 groups: control group (group A, n=10), diabetic group for 4 weeks (group B, n=14), and diabetic group for 12 weeks (group C, n=16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NE-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组:A组为正常对照组(10只),B组为糖尿病4周组(14只),C组为糖尿病12周组(16只),采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后,A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能,并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达,同时采用电泳迁移率变动分析法进行NF-κB活性检测。

Aim To observe the c-fos expression in the caudate-putman of 6-OHDA-lesioned rats after Apomorphine intraperitoneal injection and to investigate the inherent mechanism of it.

目的观察6-羟基多巴胺(6-OHDA)毁损黑质后,不同时间点腹腔注射阿朴吗啡大鼠纹状体c—fos表达情况,探讨其可能机制。

Methods Turning tests induced by apomorphine and forelimb functional tests were performed. Also, the expression of TH mRNA in mesencephalon of the rats was detected by semi-quantitative analysis of one step RT-PCR.

利用阿朴吗啡诱发旋转试验和前肢功能试验,检测PD大鼠的神经行为学特点,一管法逆转录聚合酶链反应半定量检测其中脑TH mRNA的表达。

In addition, an expression of c-fos gene could be induced in the caudate putamen by injection of apomorphine from the rats survived 2 months in the experiment group.

免疫组化染色结果表明:6-OHDA损毁7 d,损毁侧TH阳性神经元及强阳性神经元较正常侧数目明显减少(P<0.05),但阿朴吗啡不能诱导c-fos表达。

The rotations of PD rats induced by apomorphine could be inhibited by injection of CCK-8 into venous and nucleus accumbens of the lesioned sides.

静脉注射CCK-8及损毁侧伏核微量注射CCK-8可抑制PD大鼠模型由阿朴吗啡诱导的旋转行为,这种作用由CCK-A受体介导。

The apparent weight of calcium,the length and weight of the rats,and the length,weight,bone density and calcium content of the femurs were measured in every group.

将SD大鼠随机分成6组,分别喂养氨基酸钙复合物、碳酸钙和低钙饲料,通过测量相应饲料喂养大鼠的身长和体重,钙表观吸收率,以及股骨的长度、重量、骨密度、钙含量指标,观察氨基酸钙复合物对SD大鼠骨密度的影响。

Objective: To explore the relationship between corneal storage methods and rejection after corneal transplantation, and to study the roles of intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor-α in aqueous humor and serum in keratoplasty rejection in rats.

目的:探讨角膜保存时间与术后免疫排斥反应的关系,检测角膜植片的细胞间黏附因子-1(ICAM-1)表达及房水、血清中肿瘤坏死因子-α含量与角膜移植排斥反应的关系。

Abstract] Objective:To observe the effect of scald ointment made of rhubarb, dahurian angelica root, and szechwan lovge rhizome dissolved in arachis oil on scalded wound in rats.

摘要] 目的:研究由大黄、白芷、川芎等中药经加工后溶于花生油中而制成的康舒膏对大鼠烫伤的疗效。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。