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OBJECTIVE: To investigate the association between olfactory disturbance and ultrastructural mitochondrial changes in olfactory bulb neurons of aged rats in comparison with young rats.

目的:对衰老和年轻大鼠嗅球内神经元的线粒体超微结构进行对比观察,分析衰老大鼠嗅觉障碍与嗅球内神经细胞线粒体改变的关系。

METHODS: The model of LPS attack rats was established, and different doses of hydrocortisone were given to the rats.

建立大鼠LPS攻击模型,并给予不同剂量的激素,取血检测血浆ALT和AST,并观察肝脏HE染色的形态学改变。

Results: The modality of hippocampus CA1 region neurons of GLT treated AD rats was distinctly improved when compared with that of the model rats that did not receive GLT treatment.

结果:GLT能明显改善模型大鼠脑组织海马CA1区神经元的退行性变化。

The rats in blank control group were not treated by ligation and perforation after cecum separation, while the rats in experimental group and control group were performed by cecum ligation perforation to set up rat model with shock caused by septicaemia.

60只大鼠随机分为实验组、空白对照组和对照组,各20只,空白对照组分离盲肠后不做结扎、穿孔处理,实验组及对照组行盲肠结扎穿孔术建立大鼠败血症休克模型。

METHODS: Rats in each group were anesthetized with intraperitoneal injection of ethyl carbamate at dosage of 1mg/kg; then in intestinal IR group and shenfu injection group rats' superior mesenteric artery was occluded with vascular clamp for 1 hour before 2-hour reperfusion,which was not conducted in blank control group.

各组大鼠给予氨基甲酸乙酯1 mg/kg腹腔注射麻醉,肠缺血再灌注组和参附注射液组用血管钳夹闭肠系膜上动脉1 h然后再灌注2 h,空白对照组不用血管钳夹闭肠系膜上动脉。

Establishment of EAMG model in rats First, extracted rough N2AchR from crassinarke dormitory Takagi electric organ and mixed it with CFA in the same amount, and then immunized for 3 times by multiple injecting antigen liquid contained N2AchR 0.5 ml/rat on tails, feet and backs of rats.

大鼠EAMG模型建立从电鳐电器官中提取N2AchR粗提物,N2AchR粗提物和完全福氏佐剂等量混合,模型组大鼠尾根部,足垫部,背部皮下多点注射上述含N2AchR抗原液0.5ml/只,分别于实验的第1日、第14日、第30日共免疫3次。

The animal model of this method system have, the characteristics of the high fat, high leptin, high Ins, and match with the characteristic of clinical and the pathologic of simple obesity. This studies showed: The acupuncture can obviously reduce the vaule of intaking food and water of the fat rats, and lower the body weight and fat; The acupuncture can urge the diameter, area, physical volume of white fat cell contract, Brown fat tissue of fat rat group surroundings vascular containing large quantity of white fat cell, the acupuncture group was less. this showed: The acupuncture enhanced the white fat cell metabolism, and promotesed the white fat cell to convert to brown fat cell; The frequency of spontaneous electric of nerve cell of pvn of the fat rat is high, the acupuncture counld depress the spontaneous electric of nerve cell of pvn, This showed: the acupuncture can depress the appetite passing to adjust function of pvn nerve; The monoaminergic neurotransmitter of inside of the fat rat brain is disfunction, the acupuncture can rectify this disfunction, and adjust level of 5-HT, and affect appetite, and improve metabolism; The level of leptin and Ins of fat rat increased high, The level of it of acupuncture group decreased, compared with the fat group p.001, This showed: the fat rats have the defeat of leptin and Ins in the body, The acupuncture can lowering this defeat, The level of leptin and Ins of the fat rat in the brain is less than the normal group, The level of leptin of the acupuncture group is high than the fat group, This showed: the acupuncture can increase the transportation of leptin from blood to brain.

本方法所制肥胖大鼠模型具有高体重、高脂、高leptin和高Ins血症的特点,符合人类单纯性肥胖病的临床和病理特征;实验研究显示:针灸能明显减少肥胖大鼠的摄水、摄食量及降低其体重、体脂;针灸能促使白色脂肪细胞直径、面积、体积缩小,减少肥胖大鼠棕色脂肪组织血管周围白色脂肪细胞数量,显示:针灸增强了白色脂肪细胞的代谢,促进了白色脂肪细胞向棕色脂肪细胞的转化:肥胖大鼠PVN神经细胞自发放电频率增加,脑内单胺递质紊乱,针灸通过抑制PVN亢奋的自发放电,调整5-HT水平,达到影响食欲,改善代谢的作用;肥胖大鼠血中leptin及Ins水平增高,与正常组大鼠比有明显差异,提示大鼠体内存在leptin及Ins抵抗,针灸后肥胖大鼠随着体重体脂的下降,血中leptin、Ins水平降低,与肥胖组比均有显著性差异,而针灸后大鼠脑内leptin增加,与肥胖组比P.05,提示:针灸具有改善leptin、Ins抵抗及增加leptin脑内转运的作用。

We observed the variation of TBIL in obstructive jaundice rats and then made the bile duct ligation model. We also investigated the changed regularity of pulmonary capillary membrane permeability in hyperbilirubinemia, utilized the immunohistochemistry method to study the expression of pro-inflammatory cytokines in lung tissue. To search for the reason about the change of pulmonary capillary membrane permeability when the rats in BDL.

本研究应用了胆总管结扎的梗阻性黄疸大鼠模型,研究了高胆红素血症大鼠肺泡毛细血管膜通透性的变化,并利用免疫组化等方法研究了致炎细胞因子在肺组织内表达的改变,以探索肝功能障碍时大鼠肺泡毛细血管膜通透性发生变化的可能原因。

Methods The milk volume, morphology of mammary gland, serum prolactin level and pituitary acidophil number in lactation rats and the growth improvement in suckling mice were observed after administrating various dosages of Maidang Rutong Granules to lactation rats by gastric gavage .

方法哺乳期大鼠灌胃给予不同剂量的麦当乳通颗粒后,观察其泌乳量、乳腺组织形态、血清泌乳素水平和垂体嗜酸性细胞数量的变化,以及对乳鼠生长的促进作用。

Meanwhile, researchers including Inga Neumann, PhD, of the University of Regensburg in Germany have shown that pregnant and lactating rats are less prone to fear and anxiety in the face of stress than virgin rats, as measured by fewer stress hormones in the blood.

与此同时,包括德国 Regensburg大学的Inga Neumann博士在内的研究人员也通过测量血液中的压力荷尔蒙,发现怀孕和哺乳期的大鼠在面对压力时,比未曾交配的大鼠更少出现恐惧和焦虑。

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