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The experiments are conducted on adult Sprague-Dawley rats.The author investigates the discharge patterns of the non-motorneurons in the facial nucleus of rats and the alteration of respiratory rhythm to electrical stimulation of the facial nucleus in which the motor neurons are retrogradely degenarated.

实验选用健康成年SD大鼠,观察大鼠面神经核内的非运动神经元的放电样式以及电刺激面神经核对呼吸节律的影响,以探讨面神经核是否参与呼吸调节。

The study on the pathogenesis of the growth of the experimental saccular aneurysms in rats Objective To study the possible pathophysiological mechanisms of the growth of the experimental saccular aneurysms in rats.

大鼠实验性囊性动脉瘤生长塑形机制的研究目的探讨在囊性动脉瘤生长塑形过程中可能存在的血管生物学方面的病理生理机制。

The amount of middle molecule substance in plasma of normal rats and experimental schizophreniac rats changed without obvious rhythm, but during spring and summer.

正常大鼠、精神分裂症模型大鼠血浆中分子总量一年四季无明显节律变化,SP模型大鼠中分子组分有春夏季病理组分增高的趋势。

In Wistar rats, latent period of a-wave in scotopic rod response after 1-week transplantation was shorten (P.05); and latent period of a-wave in scotopic rod response after 2-week transplantation was prolonged (P.05); while latent period of b-wave in scotopic maximum response after 1-week transplantation was shorten (P.05). In RCS rats, amplitude of a-wave in scotopic rod response and photopic response after 4-week transplantation was increased (PO.05); while latent period of a-wave in scotopic rod response after 4-week transplantation was shorten (PO.05).Conclusion 1. At the age of 3 months, photoreceptors of the RCS rat degenerated completely, which leaded to the reduction of amplitudes of a-wave and b-wave.

Wstar大鼠术后1周的暗适应视杆反应,a波潜伏期缩短o叼。05);术后2周的暗适应视杆反应,a波潜伏期延长o功刀5):术后1周的暗适应最大反应,b波潜伏期缩短o功刀5卜*CS大鼠术后4周的暗适应视杆反应和明适应反应,a波振幅增大o功刀匀;术后4周的暗适应视杆反应,a波潜伏期缩短o<0卜结论1、RCS大鼠在3月龄时感光细胞变性死亡,导致FERG的a、b波振幅明显降低;视网膜下腔仍存在感光细胞外节盘膜碎屑,外丛状层缺乏感光细胞的突触末端,同时Muler细胞出现反应性胶质纤维增生。

Aim To investigate the pharmacokinetics and metabolites of scutellarin in normal rats and rats with thrombosis model induced by carrageenan.

目的 研究灯盏花素在正常和角叉菜胶致血栓形成大鼠中的药代动力学,并对其血浆样品中主要代谢物进行初步鉴定。

The bronchial asthmatic model was established by egg protein sensibilization and longterm inhalation provocation.The rats of each treatment groups were lavaged each day from the first time of provocation to execution.After 4 weeks of treatment,the rats were killed to obtain lung tissue for dyeing of HE.A computer assisted image analysis system was used to determine the thickness change of bronchus wall and smooth muscle.The contents of MMP9 and TIMP1 in lung tissue were determined by ELISA double antibody sandwich method.

除正常组外以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型,各治疗组均从第1次哮喘激发开始(造模第3周)至处死前每天灌胃给药,激发并给药4 w后处死大鼠,取肺组织HE染色,彩色图像分析仪测量支气管管壁厚度、平滑肌厚度,采用ELISA双抗体夹心法测定肺组织MMP9、TIMP1含量。

Methods At first, retrovirus vectors encoding IFN-γ or IL-4 gene were constructed. They were transfected into PA317 packaging cells by lipofectamine, PA317IFN-γ and PA317IL-4 cells were obtained. C6 glioma cells were infected with replication deficiency retrovius containing IFN-γ or IL-4 gene, cell morphology、cell growth curve and cloning efficiency assay were examined. The intracranial C6 glioma animal model was established in immunocompetent Wistar rats. PA317IFN-γ and PA317IL-4 cells were stereotactically implanted into the tumor areas alone or together. The survival of tumor bearing rats were examined, tumor volumes were measured and immunohistochemical analyses of CD4〓 and CD8〓 T lymphocyte infiltration were performed.

构建和鉴定携带IL-4或IFN-γ基因的逆转录病毒载体,将其导入逆转录病毒包装细胞PA317,通过G418抗性筛选,得到携带目的基因的包装细胞并鉴定;应用携带IL-4或IFN-γ基因的复制缺陷型逆转录病毒感染C6胶质瘤细胞,观察对C6胶质瘤细胞形态、细胞生长曲线和克隆形成率的影响;建立C6胶质瘤大鼠脑内移植动物模型,将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中,观察其治疗作用,并初步探讨其机制。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞(non-hematopoietic adult stem cells,NASC)体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同,在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓,用Ficoll-hypague分离液分离出单个核细胞后,种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养,用流式细胞仪检测细胞的免疫表型。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞(non-hematopoietic adult stem cells,NASC)体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同,在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓,用Ficoll-hypague分离液分离出单个核细胞后,种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞(non-hematopoietic adult stem cells,NASC)体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同,在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓,用Ficoll-hypague分离液分离出单个核细胞后,种植于含10%胎牛血清的DMEM/LG培养液内。

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