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Results:(1) FSN can inhibit secondary joints swelling and multi-arthritis evidently, improve the whole condition of rats; at the same time, it can also lighten the synovial inflammation and hyperplasia of lesion joints distinctly, and prevent the joint cartilage and bone from destruction; the collective efficiency of FSN is better than TWP.(2) FSN can raise AA rats low LTT of spleen to normal nearly, remedy the disorder of Th / Ts and Thl / Th2 cells balance in peripheral blood, restrain exorbitant TNF- a ? IL-1 produced by PM O , thereof exert anti-inflammatory and immunoregulation effect.(3) Inside the lesion joints, FSN can depress abnormal hyper-expression of TNF- a mRNA and NF-kB in synovial tissues, as well as advance the expression of Caspase-3 (a proteolytic enzyme of apoptosis), reduce synovial immflamination and proliferation.(4) FSN can lower the expression of VEGF in synovial tissue,reduce neogenetic veins, so inhibit the growth of pannus tissue and the damage of cartilage and bone by that.(5) The above effect of FSN are in proportion to its dosage.Conclusions: FSN has inhibitory effects on symptom and condition of experimental RA, which is better than TWP as a whole.

结果:(1)FSN有明显的抗炎作用,可显著抑制AA大鼠足跖肿胀与多关节炎,改善大鼠的全身情况,同时明显减轻病变关节滑膜炎症与增生,防止关节软骨及骨质的破坏,其综合作用优于TWP;(2)FSN还能使AA大鼠低下的脾LTT恢复至接近正常,纠正外周血中T细胞亚群Th/Ts及Th1/Th2平衡紊乱,抑制大鼠PHφ过高的TNF-α、IL-1分泌,而发挥抗炎和免疫调节作用;(3)在病变关节局部,FSN能显著抑制AA大鼠滑膜细胞异常增高的TNF-αmRNA的表达,降低滑膜组织中NF-kB表达,并增加凋亡蛋白酶Caspase-3表达,从而抑制滑膜的炎症与增殖;(4)FSN还可下调滑膜组织中VEGF表达,减少血管新生,由此抑制血管翳的形成及其对软骨和软骨下骨的侵蚀;(5)FSN的上述作用皆呈现一定的量效依赖关系,高剂量组作用优于低剂量组。

Overloaded rat models were established by using abdominal aorta constriction method. The levels of creatinine and urea nitrogen in the rats?? serum as well as those of malonaldehyde and glutathion of the rats?? nephric tissue were measured respectively.

采用腹主动脉缩窄法制备压力超负荷大鼠模型,分别测定大鼠血清肌酐和尿素氮含量及大鼠肾组织匀浆丙二醛、谷胱甘肽含量。

Healthy SD male rats were given DEG with the dose of 8 mL/kg body weight (intraperitoneal injection I.P.). The level of the serum Cr, BUN and CO2CP(CO2 Combining Power CO2CP)were tested before and after the injection the 2nd , 5th , 7th, and 14th day. The nephridial tissue of all the rats was taken for pathological examinations.

选用健康雄性SD(Sprague-Dawley)大鼠,腹腔注射8 mL/kg的DEG,于注射前和注射后第2天、5天、7天以及14天尾静脉采血检测血肌酐、尿素氮和CO2结合率(CO2CP),收集死亡大鼠及第7天和14天处死大鼠的肾组织进行病理检查。

This study is to observe the effect of of Fluvastatin in adriamycin-induced nephropathic rats. Methods The 45 male rats were randomly divided into three groups:normal control group、non-treated nephropathy group、Fluvastatin-treated group.

本课题旨在通过阿霉素肾病大鼠肾小球硬化模型的建立,并应用氟伐他汀治疗,观察其对血脂、尿蛋白的影响,以及对促进ECM降解的基质金属蛋白酶-9(MMP-9)表达的影响,探讨氟伐他汀延缓肾小球硬化的作用机制。

Methods The pregnant rats mating in the corresponding period were divided into four groups.As for the Nepionic Rats at the body growth characteristic of different days ages,the method of observing the distance and the relative distance of the anus and the vulvas when they were three to four days old was used to discriminate the sex.When they are five to eight days old,the method of observing the papillas was added to discriminate the sex.

将同期交配的孕鼠分为4组,针对乳鼠在不同日龄的身体发育特点,在3~4日龄采用观察其肛门与外阴距离和相对距离的方法、对5~8日龄再附加对乳头观察的方法进行性别鉴别对比实验。

Methods:Neuroepithelial cells were dissociated from neural tube of embryonic E12 rats and cultured in the serum of rats given BYHWD and the serum of normal controls.

取孕 12d大鼠胚胎神经管上皮细胞,分别接种在灌服补阳还五汤的鼠血清和对照鼠血清的培养基内。

Methods The rat models of neuroglioma were established,and divided into two groups. The rats in experimental group were lavaged with MMC 1 week after injected with C6 glioma cells, 10 mg.kg-1every day,the rats in control group were treated with sodium chloride at the same dose.

制备大鼠脑胶质瘤动物模型,将造模成功的大鼠随机分为对照组和实验组,实验组大鼠注射C6脑胶质瘤细胞1周后,每天灌胃给予10 mg.kg-1MMC,连续5 d;对照组给予相同体积的生理盐水。

Results: Compared with IR group , XSOL could significantly decrease the cerebral water content, the cerebral index, the brain vascular permeability in rats of acute incomplete brain ischemia-reperfusion (P.01,P.05). Cerebral tissue morphology showed that neuronic damage of rats subjected to acute incomplete brain ischemia-reperfusion was ameliorated following administration of XSOL (2.4, 9.6g/kg). Compared with IR group , XSOL could significantly decrease the content of MDA and NO in brain tissue(P.01,P.05)and increase SOD,GSH-px activity. The levels of cerebral Glu,Asp were cut down(P.01,P.05). Conclusion: XSOL shows protective effects on cerebral ischemia-reperfusion.

结果:与缺血再灌注组比较,XSOL大、小剂量组可以显著降低急性不完全性脑缺血再灌注模型大鼠的脑含水量(P.01,P.05)、脑指数(P.01,P.05)、脑毛细血管通透性(P.01,P.05);光镜下脑组织形态学显示各剂量组可不同程度的减轻急性不完全性脑缺血再灌注大鼠脑组织的神经元受损形态;与缺血再灌注组比较,XSOL大、小剂量组可以显著降低缺血再灌注大鼠脑组织中的MDA的含量(P.01,P.05),升高SOD、GSH-Px在脑组织中的活性(P.01,P.05);XSOL大、小剂量组可显著降低脑组织中NO的含量(P.01,P.05),降低脑组织中Glu、Asp含量(P.01,P.05)。

The rats in treatment group were giving tiopronin once a day at a dosage of 100 mg/kg and those in nontreatment group were fed with copper overloaded diet,however,the rats in control group were fed with normal diet.

用铜负荷饮食法制作HLD动物模型,将模型大鼠分为治疗组与非治疗组,治疗组给予MPO 100 mg·kg-1·d-1灌胃,非治疗组继续给予铜负荷饮食,对照组大鼠传统饮食喂饲。

In the third part, Ginaton and FK506 were used to improve the reperfusion of the xenograft model. When the rats were pretreated with Ginaton 30min before the transplantation, the PVBF of the xenografts at time 0, 15, 30, 45 and 60min after transplantation were 0.406±0.020, 0.357±0.025, 0.308±0.013, 0.249±0.019 and 0.205 ±0.014kHz respectively, significantly higher than those of the nontreatment rats.

为了改善豚鼠至大鼠肝移植后移植肝的微循环再灌注障碍,在接下来的第三部分中,分别进行了微循环改善剂金纳多、免疫抑制剂FK506和两者联合应用的作用研究。

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