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METHODS The models of acute inflammation in ear of mice induced by dimethylbenzene,arthroncus in rats made by carrageenin,subacute inflammation of tampon granuloma in mice and adjuvant arthritis in rats were made,and all above animal models were treated with25,50,100mg/L of the bear bile lyophilized powder injection and observed the curative effects.

方法] 分别制作二甲苯所致小鼠急性耳壳炎模型,交叉菜胶所致大鼠关节肿胀模型和小鼠棉球肉芽肿亚急性炎症模型。各组均分别给予25,50,100mg/L熊胆冻干针剂,观察疗效。

Results: The changes following hydrocortisone intervention of rats could be identified by PCA, and the kidney deficiency syndrome rats entered a state of "hyperfunction", involving a series of changes in metabolic pathway and metabolic network. The response integral area of lactic acid (δ 1.37) increased, indicating the accumulation of metabolites of lactic acid. The amount of dimethylamine (δ 2.72) also increased, indicating that the disorder of kidney function was related to kidney-deficiency syndrome. The response of creatinine (δ 3.87), asparaginic acid (δ 2.83), taurine (δ 3.44, 3.28), and hippurate (δ 7.84, 7.56, 7.64, 3.97) all decreased significantly, indicating that the hydrocortisone might cause adrenal cortex excretion injury. The response integral area of succinate (δ 2.41) and citric acid (δ 2.53, 2.68) decreased, which was usually caused by disorder of the mitochondrial function.

结果:PCA方法处理肾虚证和空白对照大鼠尿液数据显示,两组大鼠的数据可以在得分图实现分类,与对照组比较肾虚组大鼠由于氢化可的松诱导,大鼠机体进入一个过消耗后衰弱的状态,相关代谢发生显著变化,乳酸(δ 1.37)代谢发生堆积;二甲胺(δ 2.72)的含量增加,提示肾虚与肾功能异常是密切相关的;天冬氨酸(δ 2.83),牛磺酸(δ 3.44, 3.25),马尿酸(δ 7.84, 7.56, 7.64, 3.97),肌氨酸(δ 3.87)等的相对积分面积明显下降,这预示糖皮质激素可能引起了肾上腺皮质分泌功能的损害;琥珀酸(δ 2.41)和柠檬酸(δ 2.53, 2.68)是三羧酸循环能量代谢及糖酵解的中间产物,其含量的降低,通常是由于线粒体功能紊乱所引起。

The COD value in the dorsal and dorsolateral subdivisions of the PAG in the kindled audiogenic seizures-prone rats were significantly higher than that in the naive audiogenc seizures-prone rats.

用光镜观察PAG各节段各亚区切片上突触密度的变化,以期在光镜水平上了解PAG在惊厥状态下超微结构的某些变化。

Results The value of corrected optical density of the synaptophysin P38 immunoreactivity in the PAG of the naive and kindled audiogenic seizures-prone rats were significantly higher than that of the normal Wistar rats.

据此,本实验以P38免疫细胞化学反应的强度为指标,间接地对听源性惊厥易感大鼠(P77PMC)中脑导水管周围灰质(periaqueductal gray, PAG)各亚区突触的分布情况进行了研究。

Results:The three groups of P77PMC rats which experienced 30 and less than 30 times of audiogenic seizure and the Wistar control group did not display MF sprouting in dentate gyrus, however, the group of P77PMC rats which experienced 50 times of AGS was found hippocampal MF sprouting into the inner molecular layer of dentate gyrus.

结果:在全身性强直阵挛性惊厥发作次数少于30次的P77PMC大鼠以及对照的Wistar大鼠海马内均未见到MF发芽表现;而在经历50次Ⅳ~Ⅴ级听源性惊厥发作的一组P77PMC大鼠海马内观察到了MF发芽的现象,在海马齿状回内分子层内见到Timm染色呈棕黑色颗粒异常分布的MF末梢。

The main peaks of the EEG power spectra were in delta and theta frequency range. In the stage of ethanol exposure, the rats exhibited much more act of attack than the control, the alpha waves of EEG decreased, while the delta waves increased. The EEG power spectra analysis showed the delta power increased and the theta power decreased. In the stage of ethanol withdrawal, the rats showed some withdrawal behaviors such as wet dog shakes, sniffing, groomingand tonic-clonic seizures for audiogenic stimulus. Spikes and spike-slow waves were found in the EEG. In power spectra analysis, the delta power decreased while the alpha and the theta power increased, the EEG frequency band moved to the high-frequency rang. These changes were found obviously on the sixth day and disappeared on the 1 Oth day after the ethanol was removed.

本研究发现:1、对照组大鼠行为无异常,伏核EEG以α节律为主,散在出现δ和θ节律;功率主峰在δ和θ频段范围,整个过程中未发现手术对伏核EEG及脑电功率谱有影响。2、在慢性饮酒过程中,大鼠攻击性行为增加,伏核 EEG的α节律明显减少,散在出现的高幅慢活动δ波逐渐增多,功率谱分析表明δ频段功率百分比明显增加,而θ频段功率百分比明显减少,总功率减少。3、停饮后,大鼠出现湿狗样抖动、打喷嚏等撤药综合症,并且高频声音刺激能引发惊厥;伏核EEG出现棘波和棘慢综合波,并持续增多,功率谱分析显示δ频段功率百分比 5 南京医科大学硕士学位论文逐渐减少,而0和以频段功率百分比逐渐增加,总功率增加。

Results There were significant difference of autopathy between CV and SPF rats, detection rate was 94%,42.5% in livers,60%, 22.5% in kidneys,32.5%,5% in hearts and hyperplasia percentage in the lymphoid tissue around the bronchial tubes of lungs was 92.31% and 62.5% respectively; There were small focus infection and abnormal tissue changes in SPF rats.

结果普通级与SPF级大鼠均检出自发性感染性病变,且存在较大差异,检出率分别为肝94%、42.5%,肾55%、20%,心22.5%、5%,肺支气管旁淋巴组织增生程度92.31%、62.5%。SPF级大鼠依然存在比较轻微的小灶性感染和非正常组织形态改变。

Objectivc To observe spontaneous pathologic change in main organs of conventional and specific pathogen free rats, to provide necessary and important base of raising environment affecting autopathy in test animels, and to get the message of the statue of autopathy in current laboratories, to provide spontaneous pathologic change type and incidence of it for pathologic diagnosis in SPF rats.

目的观察普通级及SPF级正常大鼠主要脏器病理切片,为实验动物饲养环境对动物自身疾病的影响提供重要依据,同时了解目前实验室内动物自发病状况,为病理诊断提供SPF级饲养大鼠自发病组织学改变类型及发生率。

(1)Both the liver regeneration and apoptosis are influenced by conspicuous bilirubinemia, hepatectomy and hepatic artery resection in rats after obstructive jaundice, especially by the liver resection volume and conspicuous bilirubinemia.(2) Under the condition of conspicuous bilirubinemia, the influence of 70% PH with HAR on liver regeneration and apoptosis is severer than its counterpart of 42% in the rats. Thus, the former is not safe and should be avoided and the later can be used.

高胆红素血症时,肝切除量是影响大鼠肝切除联合肝动脉切除实施安全性的重要因素,42%肝切除联合肝固有动脉切除、胆肠再通内引流,对肝细胞再生和凋亡影响较小,安全可行;70%肝切除联合肝动脉切除、胆肠再通内引流后肝细胞再生显著受抑制,凋亡增多,死亡率高,应避免实施。

Objective To observe the therapeutic effects of the extracts from leaves of ginkgo bilobe on spinal cord injury in rats, and assess its effect on hindlimbs locomator of SCI in rats.

目的 观察银杏叶提取物治疗大鼠脊髓损伤,评估它对大鼠脊髓损伤下肢运动功能恢复的效果。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

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血精的原因很,以良性病变为主。