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The first day after C6 cells were seeded in rats bearing glioma, these rats were abdominally injected PA at the dose of 550mg/kg/d, twice a day for two weeks or DMF 570mg/kg/d in the same way.

分别在荷瘤大鼠种植胶质瘤细胞C6后第1d,对大鼠进行苯乙酸550mg/kg/d,二次/日,连续两周腹腔注射,或二甲基甲酰胺570mg/kg/d,二次/日,连续两周腹腔注射治疗。

Methods 120 healthy Wistar rats were randomly divided into 6 groups after adaptative feeding for one week: 4month control (4C) group, 14month control (14C) group, 14month JL (14JL) group, 24month control (24C) group,24month JL (24JL) group and (1424)month JL (1424JL) group, 20 rats per group, male and female were half to half in every group.

选用Wistar大鼠120只,适应性喂养1 w后随机分为6组:4月龄空白对照组(4C组)、14月龄空白对照组(14C组)、14月龄加味青麟丸组(14JL组)、24月龄空白对照组(24C组)、24月龄加味青麟丸组(24JL组)和14~24月龄加味青麟丸组(14~24JL组),每组各20只,雌雄各半。各组均按实验设计要求分别喂养后剖检。

Methods: Diabetes gastroparesis model rats were built by means of intraperitoneal injection 5% alloxanate aqu and Prepared Radix Rehmanniae once. Observed the effect of JG on rats' stomach power, serum glucose, serum motilin, cholecystokinin.

采用一次性腹腔注射5%四氧嘧啶水溶液加熟地复制糖尿病胃轻瘫模型,观察加味柴平颗粒剂对大鼠胃动力、血糖、血清胃动素、胆囊收缩素的影响。

It showed similar pattern with that in lung tissues. Conclusion: There were TGF-β1 mRNA expression in both normal rats and pulmonary fibrosis rats. AMs seemed to be the main source of TGF-β1 mRNA in lung tissues, especially at stage of alveolitis.

TGF-β1 mRNA在正常大鼠及肺纤维化大鼠中均有表达,后者表达明显高于前者;肺纤维化大鼠肺内TGF-β1 mRNA 可能主要来源于AM。

Methods: 60 SD rats were divided into 4 groups randomly, the nomal group, the model group, QZHJ group, and the hydrocortisone group. Rats were sacrificed in bdtch on the 3rd, 7th and 21st day after treatment, then the concentration of interleukin-8(IL-8) in bronchoalveolar lavage fluid and the content of transforming growth factor-β1 (TGF-β1) of lung tissue were deected, while the severity of alveolitis and fibrosis were measured.

将60只健康SD大鼠随机分为正常组、模型组、芪术合剂组和强的松组,经气管滴入博莱霉素复制肺纤维化大鼠模型,并分组予以治疗,于治疗后第3、7、21日分批处死大鼠,每组每次6只;用免疫组化法观察肺组织转化生长因子-β1的表达,并测定支气管肺泡灌洗液中中性粒细胞和白介素-8的水平。

Expressed as a fraction ofthe minimum alveolar anesthetic concentration preventingmovement in 50% of rats, the amnestic 50% effective dose (ED50)for fear to tone in control rats inhaling isoflurane and injectedwith saline intraperitoneally i.p.

用能防止50%大鼠动反应的最小肺泡浓度的一个分数来表示的遗忘音调恐惧的50%有效剂量( ED50),在吸入异氟醚且腹膜内

Methods Hyperlipemia model rats were established by feeding the rats with high fat di- et,meanwhile they were given 50,100 and 200 mg.kg~(-1) Thelenata anax and Apostichopus japonicus for 30 days.

方法采用高脂饲料饲喂法建立高脂血症大鼠模型,同时分别灌胃不同剂量(50、100和200mg/kg)的北极刺参与日本刺参匀浆,30d后测定大鼠血清TC,TG。

Rats in LPS group (n = 12) were intratrachealy administered 200 μg of LPS dissolved in 100 μl saline after anesthetization, rats in control group(n = 12 ) were given intratracheal 100 μl saline.

通过检测LPS组大鼠气管注入LPS后第2、4、7天肺组织炎症指标包括肺炎症积分、支气管肺泡灌洗液中性粒细胞计数、巨噬细胞计数、TNF-α、IL-1β及IL-8含量和气道黏液分泌指标包括肺组织阿先蓝—过碘酸雪夫阳染面积、免疫组化黏蛋白Muc5ac及逆转录-聚合酶链反应Muc5ac mRNA的表达,对LPS诱发大鼠肺炎症和气道黏液高分泌的改变进行动态观察。

Methods: Tissue of rat ovary was obtained from 30 healthy sexually mature non-mated femal SD rats. The rats were divided into two groups (oestrus group and anoestrus group). The expression of α-SMA and vimentin were detected and evaluated by immunohistochemistry and image analysis system in the theca cells of different type follicles.

分别取动情期和非动情期大鼠卵巢,运用免疫组化方法检测肌成纤维细胞的特征性标记和波形蛋白分别在这两组中各级卵泡卵泡膜细胞上的表达情况,并进行图像分析。

Methods ICH was induced in rats using stereotactic infusion antilogous blood 50μl into the caudate nucleus. The male rats were randomly divided into control group and hemorrhage group. TUNEL method was used to detect apoptosis, and immunohistochemistry method to detect the expression of Caspase-3、 Ref-1 in cerebral tissues at different time.

采取立体定向技术,将SD大鼠自体不凝血50μl注入大脑尾状核区制备脑出血模型,将大鼠随机分为正常组和出血组,分别在不同时间点断头取脑,连续切片作TUNEL、Ref-1和Caspase-3免疫组化染色。

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