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Methods: The osteoporosis model was made by male unsex rats and ANFD model was made by bipedal rats. The following indexes were observed: bone mineral density, bone metrology, fatty deposition in femoral head, blood capillary distrubution and other related biochemical indicators.

采用去势大鼠骨质疏松模型和二腿大鼠股骨头缺血性坏死模型,分别进行了骨密度、骨计量学、股骨头脂肪沉积、股骨头毛细血管分布和相关生化指标测试等试验研究。

After treatment with the above hormones, the rats were established anesthesia with urethan (1g/kg, i. p.); polyethylene catheters were inserted into the external jugular vein and the common carotid artery for drug administration, and then blood pressure was recorded. The arterial catheter was connected to a pressure transducer, and the blood pressure was displayed on the channel of BL-310 Experimental System of Biological Function through IBM computer. We assessed the changes of resting arterial blood pressure, the pressor responses of angiotensin Ⅱ(Ang Ⅱ, 0.01mg/kg), noradrenaline (NA, 0.01mg/kg) and adrenaline (Adr, 0.016mg/kg). The depressor responses of Ach (0.006mg/kg) and carotid sinus baroreflex were also discussed after treatment with the hormones in OVX rats.

用不同雌激素处理21天后,将动物用25%的氨基甲酸乙酯腹腔麻醉(1g/kg),做颈总动脉插管,通过压力换能器,将血压变化输入计算机,通过BL-310智能型生物信号显示与处理系统的压力测定模块,观察去势鼠及不同雌激素替代治疗大鼠静脉注射血管紧张素Ⅱ(AngⅡ,0.01mg/kg)、去甲肾上腺素(NA,0.01mg/kg)、肾上腺素(Adr,0.016mg/kg)的升压反应以及乙酰胆碱(Ach,0.006mg/kg)的降压反应和压力感受性反射的变化。

The protection of CRS was not affected in rats with Vagotomy but disappareds in sympathectomized rats.②...

多巴胺或异丙肾上腺素可使交感神经切除后的CRS保护作用得到部分恢复。

METHODS: 15 sprague dawley rats were randomly divided into three groups according to sample taking time after left cervical vagotomy:1 week, 1 month, 3 months, and another 5 rats without vagotomy were used as normal control.

15只SD大鼠切断左侧迷走神经,术后按取样时间随机分为术后一周、一月、三月组,每组5只;另设5只正常SD大鼠作为对照组。

Methods:Chapter one The chapter is about the effect of galanthamine on the circulating TNF in rats with abdominal infection induced by endotoxin and effects of vagus nerve on galanthamine inhibiting inflammatory response.The models of rats with abdominal infection induced by endotoxin and the animal models of bilateral cervical vagotomy were produced.

目的:研究胆碱酯梅抑制剂加兰他敏对内毒素腹腔感染大鼠循环TNF的影响,迷走神经在加兰他敏炎症抑制效应中的作用;并研究加兰他敏对内毒素刺激后巨噬细胞活化的影响,阐述加兰他敏炎症调控作用的机理。

Objective To investigate regulative effect of estradiol valerate on the expressions of estrogen receptor-alpha and -beta in the vagina of rats after ovariectomy. Methods A total of 80 adult female SD rats weighing about 340 g were randomly and equally allocated into 4 groups: normal control group, sham operation group, ovariectomized group and drug group.

目的 通过研究戊酸雌二醇对去势大鼠阴道雌激素受体(estrogen receptor, ER)亚型α、β表达的调节,探讨雌激素受体亚型与绝经期泌尿生殖道萎缩的相关性。

Objective: To investigate the changes of vasoconstrictor and vasodilator functions of isolated thoracic aorta in the rats with obstructive jaundice, so as to study the mechanism for the functional changes of the aorta in rats with obstructive jaundice.

目的:研究阻塞性黄疸大鼠离体胸主动脉收缩及舒张功能的变化规律,探讨高胆红素血症导致阻塞性黄疸大鼠离体胸主动脉收缩及舒张功能变化的可能机制。

Rats living over 96h were considered survival. To observe the protective effects of ALR gene on acute liver injury rat, thirty-six rats that were injected peritoneally with 50% CCl4 of 2ml/kg were divided into the flowing 6 group according to doses (50?g/kg and 200?g/kg ) and administration routes of pcDNA3-ALR DNA 4h after CCl4 injection: group 1, model group; group 2, ALR gene of 50μg/kg was injected venally; group 3, ALR gene of 200?

另取36只大鼠按2ml/kg腹腔注射50% CCL4,染毒后4h按不同剂量(50μg/kg 和200μg/kg )、不同注射途径(尾静脉注射、腹腔注射和联合注射)随机分为六组。1组:模型组,不注射ALR基因; 2组:尾静脉注射ALR基因 50μg/kg; 3组:尾静脉注射ALR基因 200μg/kg; 4组:腹腔注射ALR基因 50μg/kg; 5组:腹腔注射ALR基因 200μg/kg; 6组:联合注射(尾静脉和腹腔各注射ALR 基因100μg/kg)。

METHODS: A total of 30 neonatal rats were selected to prepare burn models by giving 90 ℃ boiled tap water for 3 seconds, and vibrissa follicle bulge was separated at 12, 24, 36, 48 and 60 hours postoperatively. The remained 6 neonatal rats were served as controls.

随机选取30只乳鼠,采用90 ℃水温持续3 s时间的方式制作大鼠触须部表皮组织浅Ⅱ度烫伤模型,造模后第12,24,36,48,60小时对毛囊隆突区进行分离,并进行匀浆,以同窝新生6只乳鼠正常唇部组织作为对照。

130 rats were randomly divided into five groups: 5F electroacupuncture treatment group, 50Hz electroacupuncture treatment group,100Hz electroacupuncture treatment group, the model group and the control group. 4mm defect was produced in the left sciatic nerve of each rat and silicon nerve guards were used to bridge the nerve defect not including the rats of the control group. After 10 weeks electroacupuncture treatment, gross observation, histological examination of the sciatic nerve were carried with method of HE stainning and S-100 immunhistochemical staining, Sevier-Munger stainnning. The histomorphometric examination of the triceps muscles were observed with measuring width muscle cell and the wet weight, and measuring the content of the heparin and the lactic aid of the triceps muscle with biochemistry methods.

实验方法:将130只Wistar大鼠随机分为五组:正常组、造模组、电针5Hz组、电针50Hz组、电针100Hz组,除正常组外,其余各组手术切取坐骨神经4mm后,做坐骨神经硅胶管桥接术,造成周围神经损伤模型,取患侧足三里、三阴交、环跳穴,术后第四天开始电针,每两天一次,每次10分钟,治疗10周后处死动物,取出硅胶管内新生的坐骨神经,纵切片,HE染色、S100免疫组化染色、Sevier-Munger银染,观察坐骨神经纤维再生情况,以健侧同段坐骨神经做对照;取患侧脖肠肌称取湿重,HE染色,观察腓肠肌肌细胞直径,生化测量腓肠肌肌糖元、乳酸。

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