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For normal mouse, Yi Shen Granule could prolong the swimming time of mouse, the survival in Yi Shen Granule groups was more than that in model control group in same cold condition. For low-immune mouse, The eliminated rate of carbon granule in Yi Shen Granule groups rats was faster than that in model control group rats, it showed that phagocytosis was boosted up, the absorbency of upside liquid in Yi Shen Granule groups rats was more than that in model control group rats in hemolysin mensurated test, it indicated that more antibody was come into being by giving Yi Shen Granule.

益肾颗粒可以延长小鼠游泳时间;增加在同一寒冷条件下,相同时间内小鼠的存活数;对免疫力低下小鼠,给药组动物的碳粒廓清指数增大,反映吞噬功能增强;在血清溶血素含量测定试验中,给药组动物的上清液吸收值增大,说明机体产生的抗体较多。

Objective To conduct Pharmacodynamics research of Qianliean pellet and provide the basis for the clinical medication. Methods (1) The influence on the rats with non-bacterial prostatitis of 1.5% carrageenin. Rats were divided randomly into the normal control group, the model control group, the Qianliean low-dose group (1.5 g/kg), mid-dose group (3.0 g/kg), high-dose group (6.0 g/kg), with 10 rats in each group. All groups were treated continuously for 7 days, once a day. The model control group was given distilled water, 30mins after the last medication, injects 1.5% carrageenin 0.05ml in each rat's prostate, after 24hs, behead execute the rats and record the white blood cell number and Lecithin density.

方法(1)对1.5%角叉菜胶致大鼠非细菌性前列腺炎的影响:将大鼠随机分为正常对照组、模型对照组、前列安颗粒小剂量组(1.5 g/kg)、前列安颗粒中剂量组(3.0 g/kg)、前列安颗粒大剂量组(6.0 g/kg)及阳性药前列舒乐颗粒组(2.4 g/kg)6组,每组10只,各给药组大鼠连续灌胃给药7日,每日1次,模型对照组给予等容量蒸馏水,末次给药后30 min,在每鼠前列腺内注入1.5%角叉菜胶0.05 mL,24 h后断头处死大鼠,取致炎前列腺组织,镜下记录白细胞数和卵磷脂小体密度。

Results Punctiform erosion, hemorrhage were observed by nake eyes in each groups. Epithelium cellular necrosis, defluxion, neutrophil being dominant in propria lamina and intact glandular were observed with microscopy. The erosion became severe with the successive stimulate of ethanol. There is no difference between distillate spirit and sodium chloride rats in Guth in 7th day, but Guth in distillate spirit rats was higher than that in sodium chloride rats in 30th day. PGE2, TGF-α contents in gastric mucosa of treated group increased significangly compared with control group. PGE2 showed no difference between each group in 7th and 30th day; TGF-α in gastric mucosa of distillate spirit and sodium chloride rats decreased after 1 month.

结果 茅台组、乙醇组、生理盐水组胃黏膜均肉眼可见程度不同的点状糜烂、出血;光镜下黏膜部分上皮坏死脱落,固有层以中性粒细胞为主炎性细胞浸润,腺体结构基本完整,病变随时间延长有所加重,Guth积分1周茅台组与生理盐水组差异无统计学意义,1月两者相比差异有统计学意义;茅台组、乙醇组胃黏膜组织内PGE2水平与生理盐水组相比明显增高,同时TGF-α表达增强;但PGE2水平各组间1周与1月相比差异无统计学意义;TGF-α表达强度在茅台组、生理盐水组1月后逐渐降低,差异有统计学意义。

METHODS: Liver fibrosis models were established in 24 SD rats by intragastric administration (7 g/kg) of alcohol (56°, 560 mL/L) every morning, and 10 normal rats served as controls. The rats in model group were killed by exsanguination from femoral vein at 4, 12 and 24 wk. Pathological changes of liver tissues in rats were identified by Masson staining, and the expression of VEGF mRNA was detected by semiquantitative reverse transcription-polymerase chain reaction.

56度的白酒(560 mL/L)平均以7 g/kg的剂量每日早晨灌胃一次制备肝纤维化模型,灌胃4 wk、12 wk及24 wk采用股静脉放血法分别处死大鼠,观察肝脏病理变化并采用半定量逆转录聚合酶链反应检测VEGF mRNA的表达。

It were analyzed DNA polymorphism in baby rats between outbred Wistar and SD rats with 30 microsatellites DNA loci of PCR amplification. And was breeding baby inbred rats using high and middle of Coefficient of similarity with female SD rats of F0. It was indicated that there were hybrid and polymorphism loci, there weren't homo-gene loci of F2. It were monomorphism homo-gene for 27, rate of homo-gene loci for 90%.

利用PCR扩增30个微卫星DNA位点对封闭群SD和Wistar大鼠交配繁殖的仔代鼠进行微卫星DNA多态性分析,仔代中与母代SD大鼠相似系数高的与中的进行定向交配繁殖。F2代大鼠均为杂合多态的位点,没有纯合位点;到F9代时基因纯合位点达27个,纯合基因位点率为90%。

Methods ①anti-inflammatory trial: 40 rats were randomly divided into control group, high-dose bushenlizhuo capsule group, middle-dose bushenlizhuo capsule group and low-dose bushenlizhuo capsule group(10 rats in each group).the rats in control group were given distilled water, and the rats in experimental groups were given different doses of capsule(4.0、2.0、1.0g/kg), then enhance the capillary permeability of abdominal skin of rats by using xylic acid,and the od numerical value was detected.

(1)抗炎实验:将40只小鼠随机分为补肾利浊胶囊高、中、低剂量组及对照组各10只,补肾利浊胶囊高、中、低剂量组分别给予相应剂量的补肾利浊胶囊(分别为4.0、2.0、1.0 g/kg),对照组给予蒸馏水,采用二甲苯酸致小鼠腹部皮肤毛细血管通透性增高,测定光密度值;(2)镇痛实验:40只小鼠分组及给药同抗炎实验,采用腹腔注射醋酸致小鼠出现扭体反应,纪录扭体次数并计算镇痛百分率;(3)抗前列腺炎实验:将雄性大鼠50只随机分为补肾利浊胶囊高、中、低剂量组及模型对照组及正常对照组,各10只。

Methods 64 SD male rats were fed a normal diet for one week then they were randomly divided into two groups ,one was normal group(which were 12 rats)fed normal diet, the other , which were 52 rats fed high fatty diet for 12 weeks. At the end of 13th week, 2 rats from fed normal diet group and 8 rats from fed high fat diet group were scarified to test fatty liver model .

将雄性SD大鼠64只正常喂养1周后,随机分为正常组12只饲基础饲料,实验组52只给予高脂饲料饲食(含胆固醇2%,猪油10%,基础饲料88%),13周末随机抽取正常组2只、高脂饲养8只,验证高脂饲养大鼠脂肪肝形成。

Results:(1) The rats produced amphetamine CPP after treated with 2 mgkg^(-1) amphetamine for 4 days. Ketamine and low, middle, high dose rhynchophylline all could eliminate the CPP effect, and the effect of rhychophylline was increased in a dose-dependent manner. Rhychophylline itself could not induce CPP in normal rats.(2) When compared with normal rats, the NR2B expression in nucleus accubens and amygdaloid of the model rats was obviously increased. Ketamine and middle, high dose rhynchophylline could decrease NR2B expression induced by amphetamine, while low dose rhynchophylline did not affect NR2B expression. Rhynchophylline had no effect on RN2B expression in normal rats.

结果:(1)建立了苯丙胺(2mgkg^(-1),连续4d)诱导的位置偏爱模型,氯胺酮及钩藤碱低、中、高剂量均可消除苯丙胺诱导的位置偏爱效应,随钩藤碱剂量增加其效应加强,且本身无精神依赖性;(2)苯丙胺模型组大鼠伏核和杏仁核NR2B蛋白表达增加,氯胺酮及钩藤碱中、高剂量抑制NR2B表达,低剂量及本身对NR2B表达无影响。

Methods Ten mature Wistar rats were divided into normal control group (5 rats) and adenovirus (E1, E3-Deleted and carried math1 and enhanced green fluorescent protein report gene, Ad-Math1-EGFP) scala vestibuli transfer group (5 rats). Right ears of the Ad-Math1-EGFP transfering group rats were deliveried 5μl Ad-Math1-EGFP (physical tite 2.1×10^11v.p./ml) into cochleas through the way of drilling scala vestibuli of cochlear basal turn. As a control, the normal group received nothing to inner ear. In order to estimate functional condition of vestibule and cochlea, the click-evoked potentials on the surface of the cervical dura mater, auditory brain stem response and swimming time were recorded in all rats at 7 days after treatment, and then histologic and morphologic observation were carried out after animals were sacrificed. Results All animals' morphologic observation showed that inner ear hair cells were normal after transfer.

将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein, Ad-Math1-EGFP)前庭阶导入组,每组5只,实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×10^11v.p/ml的上述腺病毒5μl,对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater, CDM-CEP)、听性脑干反应阈值检测和游泳试验,评价前庭和耳蜗功能,然后将动物处死进行组织形态学观察。

The 45 SD rats were randomly divided into normal control group with 10 rats,simple stroke group with 10 rats,stress group with 10 rats and poststroke depression group with 15 rats.

SD大鼠45只随机分为正常对照组10只、单纯脑卒中组10只、孤养应激组10只、脑卒中后抑郁组15只。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

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