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The Rats were randomized into sham group, model group and treatment group, 20 rats in each group.

大鼠随机分为假手术组、模型组、通心络组,每组20只。

Methods30 Wistar rats were randomly divided into 3 groups: the sham operation group, the simple operation group and the therapy group, 10 rats in every group.

方法Wistar大鼠30只,随机分成3组:假手术组、单纯手术组及治疗组,每组10只。

Methods:Establish the model of myocardial fibrosis in chronic stage of viral myocarditis by injecting CVB3 into Balb/c rats,divide these rats into model group、Tong xin luo low dose group、Tong xin luo medium dose group、Tong xin luo high dose group and set up normal group,after four weeks of giving medicine execute the animals and observe the ultrastructure of myocardial cell with tramission electron microscope,the quantity of myocardial collagen was observed by staining with H.E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型,随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组,并设正常对照组,用药四周后处死动物通过透射电镜观察心肌细胞超微结构;HE染色、苦味酸天狼星红染色观察心肌胶原数量;放免法测定血清透明质酸的含量;免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

The SMMC-7721 hepatoma cell line of experimental groups and control groups were cultured with the addition of sodium selenite, potassium iodide or triiodothyronine (T3). The cell quantity was checked by the MTT assay , and the cell cycle was observed by flow cytometry and levels of AFP and ALB in the culture solution were determined by RIA.The SD rats were divided into positive control group, negative control group, T3 group and mixture group (the mixture of sodium selenite, KI and T3). After two weeks of the administration of sodium selenite、 KI and T3,Walker256 cells were transplanted into the abdominal cavity. The administration was kept on for another two weeks, then observed the morbility and mortality of the rats with ascites carcinoma. At the end, the ascites were for the cells counting and the cell cycles analysising.

方 法:在体外,应用细胞培养的方法,培养SMMC-7721肝癌细胞系,分为试验组和对照组,试验组加入不同浓度Se、I或T3干预,培养一段时间后,通过噻唑蓝试验、流式细胞仪,观察细胞数量及细胞周期的变化,测定细胞培养上清液中甲胎蛋白、白蛋白的水平,观察细胞分化情况;在体内,将试验用SD大鼠分成阴性对照组、阳性对照组、T3组和混合组(为Se、I和T3混合物),首先预防性用药2周,然后给试验组大鼠和阳性对照组大鼠腹腔注射Walker256细胞,制造腹水癌大鼠模型;继续用药2周,观察各组大鼠发病率及死亡率,测量不同时期大鼠血清FT3含量,通过流式细胞仪测量大鼠腹水细胞数及细胞周期变化。

RESULTS: The mRNA levels of sodium pump α1-subunit increased and α2- and α3-subunit were unchanged in the cortex of kidneys from 1k1c hypertensive rats compared with control rats, while no change has been found for all the three subunits gene expressions at protein level.

结果:正常大鼠肾脏皮质主要表达钠泵α1亚单位,α2与α3亚单位表达较弱;在mRNA水平,1k1c高血压大鼠肾脏皮质钠泵α1亚单位表达明显较强,α2与α3亚单位表达无改变,而在蛋白水平各亚单位基因表达与对照组比较均无明显差异。

Objective To investigate the gene expression of sodium pump α-subunit in aortic smooth muscle of 1-kidney-1-clip (1k1c) hypertensive rats. Methods 1k1c hypertensive rats were prepared by partially ligating the left renal artery and removing the right kidney.

目的 探讨"一肾一夹(1k1c)"高血压大鼠动脉平滑肌细胞钠泵α亚单位各异构体基因表达的改变,为高血压发病机制的深入研究提供理论及实验依据。

The relative wet weight of the soleus muscle in tail-suspended rats was decreased by 11%(unloading 14 days) and 19%(unloading 30 days), respectively, compared with that in normal control rats.

结果 尾悬吊14、30 d后比目鱼肌的相对湿重分别下降了11%和19%。real-time RT-PCR结果表明,大鼠尾悬吊14、30 d后,比目鱼肌中MSTN mRNA表达水平均有所提高,分别是对照大鼠的2.2倍和3.5倍。

The results showed that taurine has no significant influence on testis index, epididymis index, spermaduct index, penis index and testis tectology of baby and young rats, but can make old rat testis tectology better, prevent convoluted seminiferous tubule degeneration, germ cell distortion and leydig cell atrophy. Taurine increase the level of T, E2 and LH in baby rat obviously, but has no significant effect on the level of FSH. The level of T and LH was obviously increased by taurine in young and old rats, but the level of E2 and FSH has no significant changes.

结果证明:牛磺酸对幼年、青年、老年大鼠睾丸指数、附睾指数、输精管指数和阴茎指数没有明显影响;对幼年、青年大鼠睾丸组织形态没有影响,但可明显改善老年大鼠睾丸组织形态,阻止曲细精管退化、生殖细胞变形和间质细胞萎缩;显著促进幼年大鼠 T 、 E2、 LH 分泌,而对 FSH 没有明显影响;明显促进青年和老年大鼠 T 和 LH 分泌,而对 E2和 FSH 没有明显影响。

Sphingomyelin,phosphatidylcholine,phosphstidylethsnolamine,and cardiolipinare observed in model rats and treatment rats.

观察并对比了两种模型大鼠和相应治疗组的神经鞘磷脂、磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰甘油的含量变化。

Femal rats were ovariectomized to build the model of osteoporosis, and then treated with Chinese drugs and stilbestrol respectively for 3 months. The results showed that both Chinese drugs and stilbestrol prevented the BMD, RBS and BTV from further declining in osteoporotic rats; but could not raise the BMD, RBS and BTV that had declined.

用卵巢切除的方法建立大鼠骨质疏松模型,然后对模型分别给乙烯雌酚或中药治疗3个月后发现,乙烯雌酚或中药治疗可以阻止股骨密度,抗弯强度和胫骨骨小梁体积的下降,但两种药物均不能使已经下降的股骨密度、抗弯强度和胫骨骨小梁体积升高。

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