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In short, we first found EO played an important role in hypertension in 1k1c hypertensive rats. On this basis, we put forward a plan to prepare a new depressor to direct against EO. Then we proved that ouabain polyclonal antibody can decrease significantly blood pressure in 1k1c rats.

总之,本课题首先在1k1c高血压大鼠中证实EO在高血压的发生中起着较重要的作用,在此基础上我们提出了研制一种针对于EO的降压新药的设想,并在1k1c高血压大鼠中证明哇巴因多克隆抗体具有明显的降压作用。

Forty adult female rats were divided randomly into 4 groups:① Pseudo-operation control group: no ovariotomy, no common carotid artery occlusion;② Cerebral ischemia reperfusion group: with CCAO only, no OE;③ Ovariotomized control group: with OE and CCAO, no estradiol;④ Estradiol supplement group: with OE, CCAO and estradiol supplemented (30 d after ovariotomy, estradiol was supplemented every day for 14 d). All the rats were sacrificed after 12h of reperfusion injury, 10% of brain tissue was homogenized and 10% alue was determined by UV spectrophotometer, NO content was detected, and frozen sections and NOSmRNA in-situ hybridization were performed.

取SD大鼠40只随机分为4组:①假手术对照组:不切除卵巢,不夹闭颈总动脉;②脑缺血再灌注组:作局灶性脑缺血再灌注手术,不切除卵巢;③去卵巢对照组:切除卵巢,作局灶性脑缺血再灌注手术,不补充雌激素;④补充雌激素组:切除卵巢,作局灶性脑缺血再灌注手术,补充雌激素(切除双侧卵巢30d开始补充雌激素,连续2周)。

METHODS Isolated rat lungs were used as the oxygenator. Venous blood from experimental rats was oxygenated in isolated lungs and then was perfused into the aorta of experimental rats by pump.

CPB中的肺损伤主要由体外循环中血液与非生理性管道界面接触引发的全身炎症反应以及肺的缺血再灌注损伤造成[1]。

The movement of rats' palp began at day 10.4±1.3 and the movement of rats paip approached to normal at day 18.5±1.3 in CDNF gene transfection group.

面神经损伤后经脂质体介导GDNF基因体内转染靶器官后,对受损面神经的修复有促进作用。

Methods Healthy male SD rats of different age groups were recruited(1-, 2- and 3- week groups, 1- and 2- month groups, 6- month grown group, and 18- month aged group; n=5 each). Immunohistochemistry was performed on the coronal sections of brain of the rats perfused with paraformaldehyde.

健康雄性SD大鼠,分为7天、2周、3周、1月、2月、成年(6月龄)和老年(18月龄)7组,每组5只,共35只,多聚甲醛灌注固定,冰冻冠状切片,免疫组织化学染色,光镜观察摄像,细胞计数。

Thirty male SD rats were randomly assigned into pentobarbital group and ketamine group with 15 rats in each group.

取30只雄性SD大鼠,单纯随机分为戊巴比妥组和氯胺酮组,每组15只。

Methods The rat models of ARF were made with mercury perchloride by intraperitoneal injection.Five minutes later,the rats in nmhaFGF group and model group were respectively injected with 40 μg·kg-1 nmhaFGF and normal saline by lingual vein.The rats in control group were injected with normal saline.At the time of 30 min,90 min,24 h and 48 h,the contents of blood urea nitrogen,creatinine and urine protein were detected respectively.

腹腔注射氯化汞复制大鼠急性肾功能衰竭模型,5 min后,nmhaFGF组经舌静脉注射nmhaFGF 40 μg·kg-1,模型组经舌静脉注射等剂量的生理盐水,对照组大鼠麻醉后注射生理盐水,在30 min、90 min、24 h、48 h取血检测大鼠血液中尿素氮、肌酐和尿液中蛋白含量的变化。

Fifty-four anaethetized(1% sodium phenobarbital,50mg/Kg)male or femaleWistar rats weighing 180~210g were used.They were divided into nine groups,with 6 rats in each group.

由于动物经上述模型打击后在短时内死亡,故本部分研究采用针刺法造成脑干损伤,以利于观察伤后改变的时间过程。

Neuronal death in hippocampus in epileptic rats induced by pilocarpin includes necrosis and apopto is. Oxidative stress is involved in hippocampal neuronal damage in epileptic rats induced by pilocarpine.

PILO诱导的海马神经元死亡包括坏死和凋亡两种形式,氧化应激机制参与了PILO诱导的海马神经元损伤。

In a 2003 study Pinesap and Bowling Green State University neurobiologist Jeff Bergdorf demonstrated that if rats are tickled in a playful way, they readily chirp. Rats that were tickled bonded with the researchers and became rapidly conditioned to seek tickles.

早在2003年的一项研究中,潘克塞普和保令·格林州立大学的神经生物学家杰夫·伯格多弗就曾证明,如果人们以一种十分有趣的方式胳肢老鼠,它们就会发出一种唧喳的声音。

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