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Through immunocytochemistry study, we found that the α3 and/or α5 subunits of nicotinic acetylcholine receptor are predominantly expressed in the oxytocinergic neurons. Our finding is quite different from the conclusion of the British scholar Dr. Mason's work in 1980s. We also found in this study that, the α3 and/or α5 subunits of N-AChR can also be expressed on the axons of oxytocinergic neurons, and these immunoreactive axons could be seen projecting to the median eminence and further to the posterior end of neurohypophysis. In the hypothalamus of the 7-day postnatal pup rats, the expression patterns of α3 and/or α5 subunits of N-AChR are similar to that in adult rats.

我们用免疫组化的方法发现,α3和/或α5型烟碱型乙酰胆碱受体(Nicotinic acetylcholine receptor,N-AChR)亚单位在大鼠下丘脑OT能神经元中呈优势性的表达,这与上世纪八十年代英国学者Mason类似工作的结论不一致;而且我们还发现α3和/或α5型N-AChR亚单位还表达在OT能神经元的轴突,此阳性免疫反应的轴突一直延续到正中隆起和神经垂体的末端;在新生7天大鼠的下丘脑,α3和/或α5型N-AChR亚单位的分布类似成年鼠。

According to the survival periods, the rats in the neurotomy group and neurotripsy group were divided into 3,7,14, 21,30 and 60-day subgroups with 20 rats in each subgroup.

其中,坐骨神经切断组和压榨组再各自按不同的生存时间分成3,7,14,21,30,60d6个亚组,每亚组为20只大鼠。

Methods: Forty-five adult female wister rats were divided randomly into 3 groups. The rats were received a neurotomy to bilateral sciatic nerve and subsequently reconnected with regeneration chambers.

将45只健康雌性wister大鼠,随机分成三组,切断双侧坐骨神经制成坐骨神经再生室模型,并保留断端间隙为3mm。

Most of the experiment rats whose autonomic nerves controlling uterus were cut off couldn′t pregnancy,nidation was delayed in the other rats.

选用成年雌性 Wistar大鼠,采用外科手术方法分别在妊娠前和妊娠后切断支配大鼠子宫的植物性神经,观察了切断神经对 Wistar大鼠着床的影响,并用常规组织学方法观察了子宫肥大细胞数的变化。

Methods Tissue culture was used to study the cataractous lenses of rats induced by sodium selenite or galactose. At the early stage, the content of nonprotein sulfhydryl, protein sulfhydryl, non-soluble disulfide bond, malonaldehyde and the activities of glutathione peroxidase, glutathione reductase and glutathione-S-transferase were measured in the cataractous lenses, and they were compared with that of the normal lenses. Results The two kinds of material could all induce cataract in rats, sodium selenite being more potent.

采用器官培养方法,以不同浓度亚硒酸钠及半乳糖作用于晶状体,诱发大鼠晶状体形成白内障,在培养初期测定晶状体内非蛋白质巯基(nonprotein sulfhydryl, NP-SH、蛋白质巯基(protein sulfhydryl ,P-SH)和不溶性蛋白质二硫键的含量,脂类过氧化水平,以及与谷胱甘肽代谢有关的谷胱甘肽过氧化物酶(GSH peroxidase, GSH-PX)、谷胱甘肽还原酶(GSH reductase, GSSG-R)及谷胱甘肽硫转移酶(GSH S transferase, GSH-S)的活性。

In arterioles of hypertensive rats, vasodilator responses to KCl were augmented compared with those in normotensive rats.

高血压大鼠的细动脉对 KCl 的血管扩张反应比血压正常大鼠要显著。

Methods The influence of DA on the electric activity of the pain-excited neuron in the caudate nucleus of normal rats or morphinistic rats was recorded after the sciatic nerve was noxiously stimulated.

在给予坐骨神经伤害性刺激后,记录中枢痛兴奋神经元的电活动,观察多巴胺对正常大鼠和吗啡成瘾大鼠中枢痛兴奋神经元电活动的影响。

As tail-suspended rats model can form an abnormal model of lipid metabolism as well as bone metabolism, We added three levels offish oil to the diets of rats of this model to determine the effects of n-3 polyunsaturated fatty acids on lipid and bone metabolism, so as to determine the interaction of these two chronic diseases and clarify the mechanism of these diseases.

尾部悬吊模拟失重大鼠模型能够同时造成大鼠脂质代谢与骨代谢失调,而应用此模型进行脂质代谢与骨代谢失调相关关系及膳食(n-3)多不饱和脂肪酸干预对模拟失重条件下脂质与骨代谢的影响的研究尚未见报道。本实验通过添加以鱼油为来源的不同剂量的(n-3)多不饱和脂肪酸作为膳食干预手段,研究其对模拟失重条件下构建的大鼠脂质代谢与骨代谢异常的调节作用,以加强对脂质代谢与骨代谢相互作用生理进程的深入了解为目标,为阐明中老年人群慢性疾病并发或多发的生理生化机制提供一定的实验依据。

Results The number and optical density of Bax in hippocampus of PTZ group rats was higher than that of control group rats.

然后处死大鼠取脑,免疫组织化学的方法检测海马神经元Bax和Bcl-2蛋白的表达。

Objective To observe effects of acupuncture serum of ovariectomized rats on the number of in vitro cultural osteoclast of newborn rats.

目的:探讨针刺抑制骨吸收的细胞学机制。

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