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Methods Focal cerebral ischemia and reperfusion model in male adult SD rats was established by occluding the right middle artery with 40 nylon thread blood flow blocked for 90 minutes and then reperfusion for 1 day, 7 days and 14 days.One microliter MK801 (5mmol/L and NBQX (50mmol/L) were respectively injected into the cerebral ischemic area of SD rats under stereodirectional instrument right after ischemia.

以线栓法制作成年SD大鼠局灶性脑缺血再灌注模型(阻塞90min再灌注1d、7d和14d),分别向其大脑皮质缺血区立体定向注射5mmol/L 的MK801和50 mmol/L的NBQX各1μl,用免疫荧光组织化学法检测脑缺血再灌注后成年大鼠早期大脑梗死中心区、梗死周边区和缺血对侧NG2和O4阳性细胞数量。

MethodsThe model of the middle cerebral artery occlusion in SD rats was established by nylon monofilament suture. The rats were randomly divided into treated and control groups, and they were further divided into 2 , 12 hour, 1 , 2 , 3 , 7 and 14 day reperfusion subgroups.

线栓法建立大脑中动脉缺血再灌注模型,随机分为治疗组和对照组,每组再随机分为缺血1.5 h再灌注2 h、12 h、1 d、2 d、3 d、7 d、14 d组(n=4),另取4只作假手术组。

In conclusion, Schwann cells from wallerian degenerating facial nerves of rats might bear with similar biological characteristics and functional phenotype to those from sciatic nerves in vitro, and they might be able to produce soluble NTFs for trophic support on facial motoneuron cultures. The establishment of methods for obtaining high-purity facial motoneuron cultures of rats with partially labeled fluorescence should be of significance in contribution to further investigation on facial motoneuron's response to NTFs and other affecting factors.

综上所述,本文通过面神经雪旺氏细胞体外培养条件下生物学特性、功能表型,及其对培养面神经运动神经元细胞的营养活性作用的研究,为在体外条件下研究雪旺氏细胞对面神经运动神经元的营养支持作用时,如何选择细胞来源,提供了实验依据;建立了获得较高纯度并带荧光标记的面神经运动神经元的分离和体外培养方法,为进一步研究影响面神经生长、损伤后再生修复的因素奠定了实验基础。

Methods:48 male SD rats,weighting 250-300 g,were divided into 4 groups,each group had 12 rats,they are normal control group,ischemic/reperfusion group,ischemic preconditioning group(Ⅲ group,Murry method) and adnine sulphate preconditioning group.

实验采用体重为250-300 g雄性SD大鼠48只,分4组,每组12只,即正常对照组;缺血再灌组;经典缺血预处理组;硫酸腺嘌呤预处理组。

BUN of rats taking the extract of pine needles is obviously less than that of the control group, which shows that the extract of pine needles can slow down rats metabolism rate of proteolysis, hold myotome and delay exercise-induced fatigue.

2服用松针提取液的小鼠血尿素氮含量明显低于其对照组,说明松针提取液可以降低运动小鼠体内蛋白质分解代谢速率,保持肌力,延缓运动性疲劳的发生。

The controls were only given 0.2mL corn oil injection simultaneously. The rats were sacrificed after suffering from dyspnea, and the survived rats were sacrificed at narcotism in 1 year after the first 3, 4-benzopyrene toxicosis. Lung, brain, liver, esophagus and stomach of all rates were anatomized in search of tumor.

处理后观察1年,期间出现呼吸困难时处死大鼠,首次处理后1年处死全部仍存活大鼠,解剖大鼠肺、食管、胃肠道、肝及脑等脏器,观察有无肿瘤形成,并对肿块及全部大鼠的肺组织切片进行苏木素伊红(hematoxylin and eosin,HE)染色分析。

AIM: To explore the mechanism of immune tolerance to experimental autoimmune myasthenia gravis in nasally tolerized rats and Wistar rats.

目的:探讨鼻腔耐受和Wistar大鼠对实验性自身免疫性重症肌无力耐受的机制。

While the rats in experiment group were narcotized with 25 mg/kg nembutal via abdominal cavity, and the male hepatic oval cells were inoculated (106 each point) under the liver envelope after the abdominal part was cut open. The rats received transplantation were also fed with DAB-containing food for 14 weeks to promote the formation of liver cancer.

实验组按25 mg/kg体重戊巴比妥腹腔麻醉,消毒开腹,用吸取♂Wistar大鼠卵圆细胞悬液,接种至肝脏包膜下,每点106个,之后连续喂含DAB饲料14wk,促进肝肿瘤的生成。

In addition, Immune nephelometry assay was imployed to analysis the level of rats' plasmic Fg, in the mean time, Platelet aggregation meter was used in observing the ratio of rats' platelet aggregation at 3 min. and 10 min, respectively.

实验采用酶联免疫吸附法测定各组大鼠血浆中TXB2、6-Ke-PGF1α、PGE2的水平;另外,采用免疫比浊法测定两组大鼠血浆中纤维蛋白原的含量,并同时观察各组大鼠3min,10min内的血小板聚集率。

In young rats,the glomerular filtration rat e and single nephron GFR were unchanged.In old rats the GFR and SNGFR fell significantly.

对年轻大鼠,不改变肾小球滤过率和单个肾单位GFR;对老年大鼠GFR和SNGFR显著下降。

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