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ELISA method was used for comparison. Results The positive rate of DIGFA in testing the serum from 50 infected rats was 96.0%(48/50). The negative rate of DIGFA in healthy rats was 100%(50/50). Cross reaction with Enteroclysis (13 samples) and Tapeworm (11 sqamples) was not observed. The cross reaction rate in 18 Strongyloidiasis rats was 5.6%. The sensitivity and specificity of DIGFA is comparable with ELISA (96.7%).

结果 用DIGFA检测广州管圆线虫实验感染鼠血清50份,其阳性检出率为96.0%,50份正常鼠血清的阴性符合率达100%,DIGFA分别检测蛲虫阳性鼠血清13份,绦虫阳性鼠血清11份和粪类圆线虫阳性鼠血清18份,前二者均为阴性,后者交叉反应率为5.6%;DIGFA与ELISA平行检测30份广州管圆线虫阳性鼠血清,两法符合率达96.7%。

Methods Rats were randomly divided into several groups. One group of rats received gambogic acid solution containing 0.5% CMC-Na 2 mgmL^(-1, once per day orally for six days. At the same time, the rats in the other group received 0.5% CMC-Na solution serving as blank control group. Blood samples were collect at different time point, and the plasma concentration of cocktail probe drugs was determined by HPLC.

将SD大鼠随机分组,藤黄酸组给予藤黄酸0.5%CMC-Na溶液2mgmL^(-1,以0.5%CMC-Na为空白对照,在一定的时间点采集血样,通过HPLC检测探针药物在大鼠体内的代谢情况,以评价藤黄酸对肝细胞色素P450酶的影响。

Administration of methotrexate to rats caused severe enterocolitis with 66. 7% and 16. 7% mortality within 72 hours in the first group and the second group (2% Gln), respectively. But the sucrase and aminopeptidase activities, protein content, DNA and RNA content of small intestine in rats of group 3 and group 4 were significantly higher than those in rats of group 1 and group 2. These meant that GEON enhanced digestion processes, so it can be used to improve nutritive conditions of the small intestine.

用氨甲蝶呤诱发小肠炎后,分别饲喂Gln含量不等的氨基酸营养液,实验结果表明,氨甲蝶呤诱发的小肠炎是一种较严重的应激状态,在72小时内,零剂量组大鼠的死亡率高达66.7%,2%Gln组的死亡率为16.7%,其它测定指标也相对较低,说明对小肠炎模型而言,营养液中2%的Gln含量可能偏低,第三组和第四组(结晶氨基酸组,Gln含量同第三组)的死亡率为零,体重有所恢复,蔗糖酶活、氨肽酶活、DNA和RNA含量增加,这说明,Gln活性肽营养液对小肠炎具有一定的治疗作用,可作为小肠损伤后的辅助治疗药物。

METHODS: RT-PCR was used to observe the expressions of AT2 mRNA in the cortex of rat kidney, including neonate rats (1 d), mature rats (8 weeks) and gerontic rats (20 months).

分别观察新生(1d)、成年(8wk)及老年(20mo)3组大鼠肾脏皮质中AT2mRNA的表达及其增龄性变化,并测定肾皮质中AngⅡ水平。

Methods]ninety femal sd rats,weight 230~280 gram,were rangdonmized into three groups: qiangguyin group,miacalcic group and control group.all rats were variectomized.after 10 weeks,the osteoporosis model succeed,the treatment group,contrast group were given qiangguyin,miacalcic respectively.the treatment group used qiangguyin everyday,1ml/g.the contrast group used miacalcic 0.72u/kg,everyday hyodermic.and 3,4.5,6 months after the treatment,kill 10 rats to explore.the neck of femur were cut and stined with masson golder trichrome for bone histomorphomemetric contrast group analysis.

方法]健康雌性sd大鼠90只,体重230~280g。随机分为3组,分别为治疗组、对照组和空白组,每组30只。分别采取切除双侧卵巢方法进行骨质疏松造模,10周造模成功后,开始给药,治疗组用自拟强骨饮灌胃,每日一次,每次1ml/g,对照组用密盖息,皮下注射,每日1次,每次0.72u/kg。空白组,不做处理。在给药后3、4.5、6个月每组各取10只大鼠进行检测,先测量体重,空气栓塞处死,分离股骨颈,获得股骨颈样本,经切片等处理后,显微镜下作骨形态计量学检测。

Methods Whole boold, heparinized blood, thrombin or saline were infused into the right caudate nucleus of rats in a stereotactic frame. Twenty rats were treated with hirudo powder (250mg/kg, twice daily for 5d, po). Brain water and electrolyte levels in these rats were measured at different times.

分别制作大鼠自体全血、凝血酶、肝素抗凝血、生理盐水及假手术脑血肿模型,观察不同时间血肿周围脑组织含水量,Na、K含量的变化以及病理改变;并观察水蛭粉(250mg/kg口服,每天2次,治疗5d治疗脑水肿的疗效。

Comparisons between oxytocin-induced antinociception in different hyperalgesic models The order of antinociceptive effects induced by intrathecal administration of OT is: in normal rats, in rats with inflammation, in rats with ligation .

这些结果提示,在正常及炎症痛大鼠,催产素系统和阿片系统彼此参与介导对方的镇痛效应;而在神经损伤性痛大鼠,催产素系统和阿片系统在痛觉调制上是相对独立的。

Intrathecal injection of nonselective opioid antagonist naloxone blocked the OT-induced antinociception in normal rats and in rats with inflammation; intrathecal injecion of naloxone lightly enhanced the OT-induced hyperalgesic effect in rats with ligation during hyperalgesia phase.

在正常和炎症痛大鼠鞘内注射naloxone几乎完全阻断催产素的镇痛作用,在神经损伤性痛大鼠中naloxone轻微增强催产素的痛敏作用。

To explore the influence on the expression of orexin A in the hypothalamus and orexin type 1 receptor (OX1R) in the medulla of neonatal rats after prenatal nicotine exposure, the 20 pregnant rats were divided into 2 groups randomly. Either nicotine (6 mg/kg per day) or normal saline solution (as same volume as nicotine group) was administered into the pregnant rats hypodermically from the fifth day to the end of gestation.

为了观察出生前尼古丁暴露对新生大鼠下丘脑orexin A及延髓内orexin 1型受体(OX1R)表达的影响,本实验将20只雌性成年大鼠随机均分为二组,怀孕后第5 d开始每天分别皮下注射尼古丁6 mg/kg或等量的生理盐水,直至分娩。

Observation with transmission electron microscope showed that interalveolar septa were widened with increased amount of collagen in the MP infected rats while there were no obvious abnormalities in the other two groups.(2) Positive expression of bFGF mRNA were found in alveolar walls of MP infected rats. No expression of bFGF mRNA was found in control animals. In the rats infected with MP but treated with erythromycin positive expression of bFGF mRNA was found to be scarcely distributed in alveolar walls.

2感染组动物肺泡壁见明显的bFGF mRNA阳性表达颗粒,对照组基本未见bFGF mRNA阳性表达,感染加红霉素治疗组仅见散在分布的少许bFGF mRNA阳性表达;定量分析结果表明,感染组bFGF mRNA阳性表达的积分吸光度为41.32±10.44,与对照组(0.30±0.13)和感染加红霉素治疗组(6.03±2.41)比较,差异有显著性( P <0.01)。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。