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To investigate whether atrail fibrosis occur in a pressure overload model,adult male Wistar rats was randomly divided into sham operated rats and operated rats.

1.1 动物模型及分组将体重200 g左右的Wistar大鼠42只,随机分成假手术组和手术组,两组再按不同时间(4,8,12周)分别分成三个亚组,每组7只。

Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞,然后用携带lac-Z基因的腺病毒载体转染肌干细胞,待转染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围,分别于注射后5、15d,处死大鼠取出膀胱颈及后尿道,行组织学检查及X-gal染色。

Totally 36 male Wister rats were randomly and averagely divided into three groups, and all groups were fed for 6 weeks: control group were fed with basic food; the rats in hyperlipidemia group were fed with hyperlipidemia diet; the rats in hyperlipidemia stress group were given hyperlipidemia diet, and were exposed under the different stressors at the same times, including limit, circumvolve and huddle.

36只Wister雄性大鼠随机平分为3组,均饲养6周,正常对照组喂食普通饲料;高脂组喂食高脂混合饲料;高脂加应激组除喂食高脂混合饲料外,同时给予限制应激、旋转应激、拥挤应激。

To study the different expression of Metabotropic Glutamate Receptor 1 ain the different brain areas of the kindled epilepsia models of rats induced by Coriaria LactoneMethods: 30 healthy male SD rats were randomized into 3 groups and received0.75,1.0 and 1 .25ml/kgCoriariaLactonemtramuscular injection respectively.5 same rats received saline 1.0ml/kg intramuscular injection as the control group.

研究代谢性谷氨酸受体1α(MGluR1α)在马桑内酯点燃致癫模型大鼠脑组织不同区域表达的差异及其与癫痫发生的联系。

From the histology, partial neurons of 15 d model rats got atrophy, and most neurons in 1 month model rats got cytomorphosis and atrophy, however partial neurons in 2 month model rats recovered normally.

从组织学看:15d时部分细胞萎缩,1月时大部分细胞变形萎缩,2月时部分细胞形态恢复正常。

The remaining 9 rats in control group, 16 rats in treated grouop and 15 rats in model group were decollated and obtained brains to detect the relative mRNA expression of nuclear receptor-related factor 1 (Nurr1) and muscarinic acetylcholine receptor 5(M5) byusing RT-PCR, which was the consequence of those comparing with reference geneβ-actin mRNA expression.

将对照组、治疗组、模型组其余9、16、15只大鼠,断头取脑,采用RT-PCR方法检测大鼠脑组织孤儿核受体及乙酰胆碱毒蕈碱型受体5(M5)mRNA相对于内参基因β-actin mRNA的相对表达值。

In other three groups, the left coronary artery was ligated to establish myocardial infarction models. Following 15 minutes of coronary artery deligation, the chest was opened. Rats in the cotransplantation group were treated with 250 μL (1×1010/L) BMSCs suspension + 8 mg/L Salvianolic acid B-treated EPC suspension 250 μL (1×108/L) in five points of the myocardium surrounding the deligation region. Rats in the BMSCs group received 500 μL BMSCs suspension. Rats in the model group were injected with an equal volume of cell medium.

结扎冠状动脉15 min后再次开胸,共移植组吸取骨髓间充质干细胞悬液250 μL(浓度1×1010 L-1)+ 8 mg/L丹酚酸B预处理的内皮祖细胞悬液250 μL(浓度1×108 L-1),于结扎区附近心肌组织分5点注射;骨髓间充质干细胞组同法注射单纯骨髓间充质干细胞悬液500 μL,模型组注射等量细胞培养液。

TFC could remarkably inhibit the ear edema induced by dimethylbenzene in mice at the dose of 125,250,500mg.kg~(-1), the swelling of rats hind paws induced by carrageenan and primary inflammatory reaction in AA rats at the dose of 84,168,336 mg.kg~(-1) and reduce the weight of granuloma induced by cotton in rats at the dose of 84,168,336mg.kg~(-1), respectively.

TFC的免疫调节作用体外研究发现,TFC0.005,0.05,0.5,5,50 mg·L~(-1可明显提高环磷酰胺(cyclophosphamid,Cy)诱导免疫功能低下小鼠的脾淋巴细胞增殖反应,而对正常小鼠的脾淋巴细胞增殖无明显作用。

Experimental study indicated: the flavone of rhizome drynaria can improve the cytoactive of marrow stroma cell of rats, promote its multiplicative division, and protect the marrow stroma cells' apotosis with lipidsion.The flavone of rhizome drynaria's serum can extently protect the osteoblast apoptosis of rats induced by TNF-α and the osteoblast senescence of rats induced by t-BHP.

实验研究表明:骨碎补总黄酮能够提高大鼠骨髓基质细胞的活性,促进其增殖分裂,并对脂质化大鼠骨髓基质细胞凋亡具有保护作用,而且骨碎补总黄酮含药血清对TNF-α诱导的大鼠成骨细胞凋亡和t-BHP诱导的大鼠成骨细胞衰老均具有一定的保护作用。

Results The control group had no seizures. NMDA-induced automatisms and emprosthotonus in (12-13)-day-old rats were elicited while automatisms were only elicited by NMDA from 14-day-old rats to 25-day-old rats without emprosthotonus.

结果:对照组无癫痫发作,12~13日龄Wistar乳鼠引出甩尾等自动症及前弓反张发作,14~25日龄Wistar乳鼠只有自动症而没有前弓反张发作。

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