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B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.
体细胞胚胎发生研究的结果表明:(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料;(2)在所试验到的MS、MS1/2(将MS的所有成分均减半)、WPM、DKW、B〓、SH等六种基本培养基中,MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基;(3)水曲柳的体细胞胚胎发生以直接发生为主,体细胞胚主要来自于从合子胚分离的完整子叶;(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响,诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2,5,10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0,2mg/L+TDZ 0.05mg/L;(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力,少数愈伤组织可以分化出体细胞胚;(6)愈伤组织的诱导也受培养基和激素配比的显著影响,最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2,5mg/L+TDZ0.05mg/L;(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力,此时种子尚未成熟,胚乳已呈固态,种胚的各个部分已分化完全,种胚体积占胚腔的大约2/3;(8)在各自综合的最适条件下,完整子叶的体细胞胚诱导率可达33.68%,下胚轴的愈伤组织诱导率可达90.7%。
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The maxima of low-temperature internal friction peaks plot a symmetrical peak-like curve at logarithmic frequency spectrum and the peak-like curve shifts toward high frequencies with the increase of heating rate, indicating that phase transition relaxation time decreases; the maxima of high-temperature internal friction peaks exhibit a linear relation to the reciprocal frequencies, and as the heating rate increases its slope rises, which implies that the heating rate drives the normal movement of phase interface further in low-frequency measurements.
低温内耗峰峰值与对数频率呈现峰形函数关系,随升温速率的增加,频率峰向高频方向移动,相变弛豫时间减小;高温内耗峰峰值与倒数频率成直线关系,随升温速率的增加,直线斜率变大,表明低频测量时升温速率对相界面法向运动的驱动作用更强。
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The most widely used bank-to-bank lending rate, the London interbank offered rate, which has risen sharply, is also used to calculate interest rates on credit cards and many adjustable-rate mortgages.
运用最广泛的伦敦银行同业拆息近期急剧上扬,该指标也被用于计算信用卡和许多可调息按揭的利率。
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" Floating rate note: Note issued from five to fifteen years with a fixed spread, usually over the six month London Interbank Offered Rate, normally with provision for a minimum interest rate."
浮动利率票据:这种票据期限为5到15年,利率通常为6个月伦敦同业拆放利率加固定利差,并有最低利率规定。
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Difference in N uptake responsiveness was linked to their difference in N uptake rate and root characteristics. N uptake rate of high-responding cultivars increased significantly with increasing N rate, while their total root length exhibited a comparatively lower variation.
对氮响应度高的4个水稻品种的平均吸氮速率随着供氮水平的增加其增幅显著,而其总根长的变化幅度则较小。
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The formation of our 1D nickel wires can be ascribed to the cooperative effect of the reaction rate and magnetic field.The reaction rate in the system is controlled mainly by adjusting the reactant concentration when other reaction conditions are fixed.When the concentration of Ni~(2+) is low,the reaction rate is correspondingly slow,so the complex[Ni(N_2H_4)_x]~(2+) preferentially migrates to the magnetic line of force since paramagnetic metal ions are attracted toward the maximum field.
当反应物Ni~(2+)浓度较低时,反应速率相对较慢,[Ni(N_2H_4)_x]~(2+)优先选择迁移到磁力线附近并且沿着磁力线方向排列,导致电极电势增大,于是[Ni(N_2H_4)_x]~(2+)的化学还原反应也是沿着磁力线发生,结果导致了镍纳米线的形成。
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The results showed that explant sterilized in 0.1% aqueous mercuric chloride for 5 min had better effect, and the contamination rate was 31%, the germinating rate was 79%, inducement rate was 65.2%.
结果表明:用0.1%氯化汞对青岛百合叶片消毒5 min时效果最好,污染率为31%,萌动率为79%,诱导率为65.2%。
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Recycling rate on benzene with content of 0.5,21.1 and 200μg distilled by methyl alcohol is separately 95%,94% and 91%; recycling rate of 0.5,41.6 and500μg is separately 99%,99% and 93%; recycling rate on dimethylbenzene of 0.5,34.4 and 500μg is separately 101%,100% and 90%.
用甲醇提取法对苯的含量为0.5,21.1和200μg的回收率分别为95%,94%和91%,甲苯的含量为0.5,41.6和500μg的回收率分别为99%,99%和93%,二甲苯的含量为0.5,34.4和500μg的回收率分别为101%,100%和90%。
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The pathogenic bacteria of AV was primarily Gram-positive aerobic bacteria,with the detection rate of 80% including Staphylococci aueas,Enterococcus faecalis and E.coli;low rate of B.streptococcic was detected.The pathogenic bacteria of BV was primarily Gram-negative anaerobic bacteria,while the rate of Gardnerella was detected as less than 40%.There were 8 cases with of AV concurrent infection in 48 cases of BV infection,and 6 cases with BV concurrent infection in 31 cases of AV infection.Conclusion Microphyte profile analysis indicated that patients infected by AV were susceptible to anaerobic bacteria such as Gard...
正常女性阴道中以乳酸杆菌为主,产H2O2乳酸杆菌的检出率89%,AV的致病菌主要是革兰阳性率需氧菌、检出率可达80%,其中金黄色葡萄球菌,粪肠球菌和埃希氏大肠菌较为常见,B族链球菌的检出率较低;BV的致病菌主要是厌氧菌,以革兰阴性厌氧菌的检出率最高,加德纳菌的检出率不足40%。48例BV患者中有8例合并AV感染,31例AV患者中有6例合并BV感染。
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It turned out reproduction rete of chrysanthemum was raised,the regular,non-plant diseases and insect pests could be offered to the market. Shoot tip were used as explants to study the effects of different sterilization methods, illumination intensity and concentrations of 6-BA and NAA on shoot propagation. Rooting with perlite, vermiculite and river sand instead of agar was studied. In the process of preliminary multiplication, the effect of using 0.1% HgCl_2 in sterilization of explants was better than using saturated bleaching solution. The highest multiplication rate was found under whole illumination. MS media with 6-BA from 0.2 mg·L~(-1) to 5 mg·L~(-1) and NAA from 0.05 mg·L~(-1) to 2 mg·L~(-1) were tested and as a result, the highest differentiation rate and the multiplication rate was reached with 0.2 mg·L~(-1) 6-BA, 0.05 mg·L~(-1) NAA.
本实验以菊花的茎尖为外植体,研究了不同灭菌方法,光照强度,全光照、半光照和弱光处理以及不同6-BA和NAA浓度及配比对于试管苗增殖的影响,并研究了珍珠岩、蛭石和河砂等琼脂替代物对于菊花生根的影响,结果表明:在初代培养物建立的过程中采用0.1%升汞进行表面灭菌的效果好于饱和漂白粉的灭菌效果;在全光照条件下外植体的增殖倍数最高;在试管苗增殖培养的过程中,以MS为基本培养基,并在培养基中添加0.2~5mg·L~(-1)6-BA和0.05~2mg·L~(-1)NAA,其中0.2mg·L~(-1)6-BA和0.05mg·L~(-1)NAA最适于外植体的分化和增殖,其分化率为100%,增殖倍数为12。
- 相关中文对照歌词
- Third Rate Romance
- Third Rate Romance
- My Heart Rate Rapid
- Distance Equals Rate Times Time
- The Going Rate (My Fix)
- 推荐网络例句
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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.
。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。
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Go down on one's knees;kneel down
屈膝跪下。。。下跪祈祷
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Nusa lembongan : Bali's sister island, coral and sand beaches, crystal clear water, surfing.
Nusa Dua :豪华度假村,冲浪和潜水,沙滩,水晶般晶莹剔透的水,网络冲浪。