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In this study, the effect of nCDHA on chondrogenic differentiation of rat adipose-derived adult mesenchymal stem cells in alginate gel was evaluated. nCDHA/alginate composites containing different amounts of nCDHA were prepared and used to encapsulate rat ADAS. The chondrogenic differentiation of ADAS was enhanced after 7 days and the total collagen content increased after 14 days at 500 ppm of nCDHA.

中文摘要本研究先制备一系列nCDHA/alginate奈米复合材料以三维培养方式,探讨大鼠脂肪干细胞经过ㄧ周与二周培养后,软骨分化基因及软骨基质分泌(collagen,GAG)进行评估,结果发现500 ppm nCDHA可增加第7天软骨基因分化与第14天胶原蛋白增生。

Objective To evaluate the effects of newborn rat hepatocyte intrasplenic transplantation combined with rat augmenter of liver regeneration injection in treating rats with acute hepatic failure. Methods Acute hepatic failure was induced in rats using D-gal (1.2 g/kg).

目的 探讨新生大鼠肝细胞脾内移植联合重组鼠肝再生增强因子治疗大鼠急性肝衰竭的疗效与免疫学机制。

Reach what the SPF class small rat that its feed undertakes bacterium and helminth 1 year to monitor to this system continuously, live to what bandicoot undertakes imitate cuts off the power and bacterioscopy, be in to raising the comparative test that the small rat of SPF class KM of traditional protective screen system and IVC system has immune index.

连续1年对该系统及其饲养的SPF级小鼠进行细菌和寄生虫的监测,对大鼠进行模拟断电的生存和细菌检查,并对饲养在传统的屏障系统和IVC系统的SPF级KM小鼠进行免疫指标的对比实验。

The profile of liver CYP450 induction by DEX in male rat After 4 daily i.p. administering of 0,25, 50 and lOOmg/kg/d DEX in male Wistar rat, it was determined the total CYP450 content, the mRNA and protein expression levels of CYP3A1, CYP3A2 and CYP2B1/2, and the ERD (CYP3A catalytic activity), PROD (CYP2B catalytic activity) and BROD (total CYP450 catalytic activity) in livers.

DEX对雄性大鼠肝脏CYP450的诱导效应雄性Wistar大鼠腹注0、25、50和100mg DEX/kg/d诱导处理4天后,测定大鼠肝脏总CYP450含量,CYP3A1、CYP3A2和CYP2B1/2的mRNA及蛋白表达水平,肝脏红酶素脱甲基酶(ERD,CYP3A活性),苄氧基试卤灵脱乙基酶(PROD,CYP2B活性),苯氧基试卤灵脱乙基酶(BROD,总CYP450活性),结果表明,CYP450含量、ERD、PROD和BROD在DEX多次诱导后都有升高,CYP3A1 mRNA表达水平、蛋白含量和酶活性有明显的升高,剂量效应关系明显;CYP3A2蛋白也有明显升高,而其mRNA表达水平却没有变化,提示CYP3A1和CYP3A2的诱导机制可能不同。

The methods of purification and reconstitution of Ca〓-Mg〓-ATPase were set up and Ca〓-Mg〓-ATPase from both spontaneously hypertensive rat and control rat were isolated, purified and reconstituted in the study.

建立了质膜钙泵的分离纯化方法和磷脂重建方法并用这些方法成功地分离纯化了正常大鼠和自发性高血压大鼠红细胞膜钙泵,而且将其重建于人工脂质体上。

Discovered inside the crater of an extinct volcano, the Bosavi woolly rat is not a giant guinea-pig-like rodent like the capybara, but an actual member of the rat family.

发现与一个死火山的火山口。这个博萨维绒毛鼠不是大型几内亚像猪的啮齿目动物,而是真正的鼠科动物。

The homology of the Myostatin sequences was analyzed among species. The results showed that the mature region DNA for Myostatin of Jinhua pig has the similarity of 100%% 97%, 95%, 95%, 93%, 93%, 93%, 89%, 90%, 89%, 87%, 87%, 86%, 86%, 86%, 85% and 76% compared with that of pig, horse, human, monkey, cow, hare, goat, sheep, house mouse, rat, goose, turkey, chicken, pigeon, Xiaoshan chicken, Fan duck and zebrafish respectively. The mature region DNA for Myostatin of Xiaoshan chicken has the identity of 99%, 97%, 93%, 92%, 92%, 87%, 86%, 86%, 86%, 86%, 85%, 85%, 85%, 84%, 84%, 83% and 74% compared with that of chicken, turkey, pigeon, goose. Fan duck, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively. The mature region DNA for Myostatin of Fan duck has the homology of 91%, 92%, 92%, 90%, 95%, 84%, 84%, 85%, 85%, 85%, 83%, 81%, 81%, 83%, 81%, 82% and 73% compared with that of chicken, Xiaoshan chicken, turkey, pigeon, goose, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively.

将测序结果与GenBank上其它物种的相应序列进行同源性分析,结果显示,金华猪Myostatin与哺乳动物猪、马、人、猴、牛、兔、山羊、绵羊、小家鼠和大鼠的同源性分别为100%、97%、95%、95%、93%、93%、93%、89%、90%和89%,与鸟类鹅、火鸡、鸡、鸽子、萧山鸡和番鸭的同源性分别为87%、87%、86%、86%、86%和85%,与鱼的同源性为76%;萧山鸡Myostatin与鸟类鸡、火鸡、鸽子、鹅和番鸭的同源性分别为99%、97%、93%、92%和92%,与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为87%、86%、86%、86%、86%、85%、85%、85%、84%、84%和83%,与鱼的同源性为74%;番鸭Myostatin与鸟类鸡、火鸡、鸽子、鹅和萧山鸡的同源性分别为91%、92%、90%、95%和92%,与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为84%、84%、85%、85%、85%、83%、81%、81%、83%、81%和82%,与鱼的同源性为73%。

Result: The shoe gum that contains toluene and fourth ketone its LC50 lowest, contain ester only kind shoe glue LC50 is highest; The LC50 of female Xiaobai rat is less than male Xiaobai rat; The LC50 when the temperature of shoe glue environment of akin composition is high is low; The symptom that the shoe gum that contains toluene and fourth ketone produces after contamination of small white rat is much, restore time to grow, pathology sees drop of liver lung haemorrhage.

结果:含甲苯与丁酮的鞋胶其LC50最低,只含酯类的鞋胶LC50最高;雌性小白鼠的LC50小于雄性小白鼠;同种成分的鞋胶环境温度高时LC50低;含甲苯与丁酮的鞋胶小白鼠染毒后产生的症状多、恢复时间长,病理可见肝肺出血点。

The results showed that at the 4th week, high calorie diets induced the UCP2 mRNA expression in rat white adipose tissue , but it had no effect on the level of UCP2 mRNA in rat brown adipose tissue and skeletal muscle and the gene expression of UCP3 and GLUT4 in rat skeletal muscle.

通过本实验研究发现:喂饲4周时,高能饲料就能诱导大鼠白色脂肪组织中UCP2 mRNA表达,但不影响大鼠褐色脂肪组织与骨骼肌中UCP2 mRNA水平;也不影响大鼠骨骼肌UCP3和GLUT4基因表达。

Bcl-2 mRNA was detected by RT-PCR in all cells;(2)After normal rat and bile duct-ligated 14d rat hepatocytes were added 100 μmol/L GCDC and kept for 24 hours, cells were evaluated by FCM and TUNEL. Results:(1)Normal rat hepatocytes did not express Bcl-2 by RT-PCR technique. Bcl-2 was expressed in 7-,14-,21- day BDL rats.

分离对照组及实验组14 d组大鼠的肝细胞行原代培养后,使用100 μmol/L甘氨鹅脱氧胆酸钠作用于两组肝细胞24 h后,用FCM法测定肝细胞的凋亡比率,用TUNEL技术进行肝细胞凋亡的原位检测。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力