查询词典 rat-tat

Had rarely or never used protective;(11) MiceGrayAuto had realized the automatic processing of video data from the sexual preference observation system of rats, and MiceGrayManu had accomplished the analysis of images of sexual behaviors of rats;(12) Peripheral given of high dose androgen to female rats before its brain sexual differentiation had caused masculinization and defemininization in female rats, therefore led to homosexuality, and based on the results, we had established the female rat homosexual model;(13) Peripheral given of estrogen to male rats before its brain sexual differentiation had little effects on the sexual preference and sexual behavior of adult rats;(14) Peripheral given of estrogen antagonist to male rats before its brain sexual differentiation had markedly reduced masculine sexual behavior and interfered male sexual preference, but had no effect on feminine sexual behavior, and based on such results, we had established the male rat homosexual model, however the model have to be improved for better sensitivity;(15) The volume of sexually dimorphism nucleus of the preoptic area of the high dose androgen-treated female rats was significantly greater, while on the other hand the volume of AVPV of which was significantly lesser, than the female rat control.

并且有17.79％的男性同性恋者在1年内与异性保持着性关系。但在与异性阴道性交时，绝大多数（92.51％）偶然或不使用安全套；(11)MiceGrayAuto软件实现了大鼠性偏爱视频数据自动处理功能，MiceGrayManu软件实验了大鼠性行为图象分析功能；(12)大脑性分化前高剂量的雄激素有促雌鼠雄性化和去雌性化的作用，并表现为同性性倾向，建立了雌鼠同性恋模型；(13)在雄鼠大脑性分化前外周给予雌激素对成年后的性偏爱和性行为未产生明显影响；(14)在雄鼠大脑性分化前外周给予雌激素拮抗剂明显削弱雄性性行为，阻碍了雄性性偏爱，但雌性行为无明显变化，雄鼠的同性恋模型还有待进一步调整和改进：(15)高剂量雄激素处理的雌鼠SDN-POA体积明显大于对照雌鼠组，而AVPV体积明显小于对照雌鼠组。

Part 4: Brain-derived neurotropic factor genetically modified olfactory ensheathing cells of the neonatal rat Objective To culture the olfactory ensheathing cells from the neonatal rat and modify them genetically by human Brain-derived neurotropic factor to increase the formation of neurophic factors. Methods The OECs were dissociated from the neonatal rat olfactory bulbs and purified with time-speed difference stick.

第四部分：脑源性神经营养因子基因转染新生大鼠嗅鞘细胞目的为提高嗅鞘细胞(Olfactory ensheathing cells，OECs)分泌脑源性神经营养因子(BDNF，Brain-derived neurotropic factor)的能力，探讨将外源性BDNF基因转染入OECs，为今后采用BDNF基因修饰的OECs移植治疗SCI奠定基础。

DST showed that the pretreatment with dexamethasone can not repress the total number of CRF immunoreactive neurons in the rat model induced by CUMS, by contrast with a normal repression in the number of CRF neurons in rat induced by acute stress. In brief, the rat model induced by CUMS represented decreased body weight, obvious depression-like behavior, hyperactivity in HPA axis and nonsuppression on the DST, which fits the criteria and neurobiological feature of depression.

简而言之，CUMS诱导的大鼠模型有典型的抑郁症样行为和表现、HPA轴活性升高、DST的失抑制，符合抑郁症模型的行为学标准和主要神经生物学特征，是抑郁症稳定可靠的模型，也是研究应激诱导的糖皮质激素升高所带来的神经生物学效应的良好模型。

Objective To establish rat model with polycystic ovary with two different methods:testosterone plus human chorionic gonadotropin and progestogen plus HCG,compare the rat models induced by testosterone plus HCG with the patients with polycystic ovary syndrome in the aspects of ovarial morphous and gonadal hormone,and investigate whether testosterone with HCG is the effective method to establish rat model with polycystic ovary syndrome,than compare two methods(T+HCG and P+HCG)finally to draw a conclusion if there are any difference or similary between the two methods and which one is more ideal and convenient.Methods 60 SD female rats of 23 days were divided into three groups in random.

目的 本实验用睾酮联合绒毛膜促性腺激素及炔诺孕酮联合HCG两种方法分别建立多囊卵巢大鼠模型，将模型鼠的卵巢形态及血清激素方面改变与人类多囊卵巢综合征的病理改变加以比较，探讨睾酮联合HCG是否是建立多囊卵巢大鼠模型的有效方法，并比较两种造模法是否存在异同点，判断哪一种造模方法更为理想及简便有效。

Object: The project is on the basis of chronic alcoholic myopathy model of rat, to dynamically observe the change of NF-κB and MMP-2, MMP-9 in muscle cell, observe the function in the mechanism of alcohol myopathy, move forward a single step to explain the pathogenesy of this disease from gene and protein leve. We also provide a helpful platform for other metabolic myopathy. Methods:(1) 80 health, male, Sprague-Dawley rats(weight 180~200g), were chosen to sep up the CAM rat myopathy model ,to observe the rat bodyweight, the change of praxiology and the patho-change of muscle tissue.

目的：本实验旨在建立大鼠慢性酒精中毒性肌病模型的基础上，动态观察NF-κB及MMP-2、MMP-9在酒精中毒性肌病发展中的表达、变化情况，探讨其在慢性酒精中毒性肌病发病过程中的作用，从基因和蛋白水平进一步阐述该病发病机制，为慢性酒精中毒性肌病的临床防治提供理论依据，同时对于其他代谢性肌病的研究也提供一个平台。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Except on the testis tissues,the expression of ZFY gene were found on the kidney tissues of human embryo or rat, brain vesicles〔rat embryo 13 days〕,and sclerotome cells〔rat embryo 13 days〕 It is possible that because kidney and genital system come from same origin,ZFY gene expressed on the kidney tissues too, the expressive function of ZFY gene on the embryonic brain vesicles and sclerotome cells is not yet clear.

除了在睾丸组织上表达外，ZFY基因还在肾脏组织及鼠胚脑泡(胚胎13天)，生骨节细胞(胚胎13天)上表达。在肾组织上表达可能是肾脏与生殖系统起源相同有关。在胚脑泡及生骨节细胞上表达其作用不清楚。

Others believe that marrying off the rat is a polite way of exorcizing rat-afflicted disasters. The old-time New Year paintings had a theme of "Rat's Taking a Wife", with a merry wedding parade of lovely rats.

旧时年画，就有&老鼠娶亲&，画面上一支喜气洋洋的迎亲队伍，清一色的老鼠，个个十分可爱，而在这支队伍的最前面，却偏偏蹲着一只笑容可掬的猫儿。

Rresults from those studies can be summariazed as follow:(1) A preliminary study no correlationship between the UV-VIS reflection spectrum of tomato fruit surface and the lycopene amount of tomato fruit was undertaken. Based on observation from this study, a damage-free and in-situ detection technique of lycopene amount from tomato was developed with UV-VIS reflection spectroscopy;(2) The geometrical isomers of lycopene were separated successfully on C30-HPLC-PDA-ELSD. The absorption coefficients of Z-isomers were consequently dedermined. The geometrical isomer composition of lycopene could therefore be assessed. Those efforts formed a solid base to identify the natural product from synthetic compound of lycopene;(3) Lycopene resource from the fruit of Autumn oliver was proved to be available for industrial application. An extraction methos of lycopene from the fruit of Autumn oliver by supercritical carbon dioxide was developed at laboratory scale;(4) A method to extract lycopene by supercritical liquid with improved efficiency was developed; Data from this investigation suggested that a proper raw material pre-extraction process was important for a better extraction efficiency;(5) Variation in the geometrical isomer composition of lycopene during its metabolish in rat was assessed. A large amount of Z-isomers were observed. This variation took place in serum;(6) The absorption rate and accumulation in serum of natural lycopene in rat were assessed;(7) Ability to quench singlet oxygen by different geometrical isomers of lycopene was compared;(8) It was detected that whether natural lycopene had functions to regule blood-lipids and LDL antioxidation;(9) A C31 degradation piece of lycopene was found in the prostate gland of rat.

研究结果包括：[1]应用UV-VIS反射光谱法建立了番茄果实中番茄红素含量的无损伤、现场检测技术；[2]应用C30-HPLC-PDA-ELSD技术实现了对番茄红素几何异构体的分离，测定了不同顺式异构体的吸光系数，建立了天然番茄红素几何异构体组成的检测方法，为区分和鉴定人工合成品和天然产物奠定了基础；[3]证实了秋橄榄果实中的番茄红素资源具有工业开发价值，并建立了超临界CO_2萃取的实验室方法；[4]探索了提高番茄红素超临界流体萃取效率的方法，证明了物料前处理对提高萃取效率起了极其重要的作用；[5]观察了番茄红素在大鼠体内几何异构体组成的变化情况，发现了大量顺式异构体的存在，并证实了几何异构体组成在血清中发生了显著变化；[6]测定了天然番茄红素在大鼠体内的吸收率和在大鼠血清中的动态积累规律；[7]比较了不同番茄红素几何异构体淬灭单线态氧的能力；[8]检测了天然番茄红素是否具有调节血脂作用和抗低密度脂蛋白氧化的功能；[9]首次发现大鼠前列腺内存在番茄红素C31降解片段，并证实其仍具有淬灭单线态氧的能力。

Antinociception and VGSC-modulation of BmK IT2 and BmK AS—1. The results from nociceptive flexion reflex and plantar radiation heat showed: BmK IT2 (a depressant insect-selective neurotoixn) and BmK AS-1 (a new-type β-1ike neurotoxin) have significant antinociceptive effects on rat peripheral nerve system. The antinociceptive effects of BmK IT2 on the carrageenan-inflamed rats were significantly stronger than those on the normal rats. Whole-cell patch-clamping showed: Both TTX-S and TTX-R Na〓 currents induced from rat DRG neurons were significantly inhibited by BmK IT2 and BmK AS—1, and BmK IT2 was more sensitive to TTX-R than TTX-S component. All the results suggested: the peripheral antinociception of BmK IT2 and BmK AS—1 might be attributed to the inhibitory effects of them on the TTX-S and TTX-R Na〓 channel located on the peripheral ends of rat DRG neurons with small diameter.

中文题名特异性钠通道调制剂－长链蝎神经毒素的药理功能研究副题名外文题名 The study of pharmacological function of the specific modulators on voltage-gate Na+ channel-long chain scorpion neurotoxin 论文作者谭智勇导师吉永华研究员学科专业生理学研究领域\研究方向学位级别博士学位授予单位中国科学院上海生命科学研究院学位授予日期2002 论文页码总数93页关键词神经毒素毒理学电压门控钠通道东亚钳蝎钠通道调制剂馆藏号BSLW /2003 /R338 /22 本论文工作利用细胞和行为药理技术系统地研究了东亚钳蝎长链神经毒素的抗伤害性效应、对电压门控钠通道的电生理调制作用、改变胞内离子浓度的效应，并分析了相关的药理功能机制。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。