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Experimental study on culture and identification of neural stem cells from both ependyma of fetal rat and bone marrow of adult rat[J].

胚鼠室管膜及成鼠骨髓神经干细胞分离培养及分化鉴定的实验研究[J]。

INTERVENTIONS: Embryonal 14-day SD retina was transplantedin-between the epithalamus and hypothalamus of the midbrain in P1 rat and the right eye of the P1 rat was removed simultaneously. The development time of TP on the 9th day after operation was corresponding to the normal retina on the 1st day after born, which was then recorded as TP1, and so on. The development and differentiation was shown by histochemical method.

干预:用胚14 d SD大鼠视网膜移植至P1大鼠中脑左侧上、下丘间,同时摘除P1鼠右眼,术后第9天移植视网膜的发育时间与生后第1天正常视网膜相当,记为TP1,余类推,应用组织化学方法显示神经元的发育和分化。

To investigate the role of gene Gli3 in idiopathic congenital talipes equinovarus, we constructed the Gli3 luciferase reporter gene expression vectors to analyze the promoter activity of the rat gene Gli3. The regulatory element in the promoter region of the rat Gli3 was predicted using P-Match software and further verified by ChIP experiment.

为了探讨人类GLI3基因在单纯性马蹄内翻足(Idiopathic congenital talipes equinovarus, ICTEV)发生时所起的作用,文章构建了大鼠Gli3基因启动子区域荧光素酶报告基因表达载体来分析Gli3基因启动子的活性,并利用P-Match软件预测大鼠Gli3基因启动子区域可能的调控元件,应用ChIP实验加以验证。

Methods:①Pharmacodynamics observed the effects of The root and leaf of Panicled Fameflower on swimming time of mouse, and physiological function, IL-2 IgA IgM IgG C3 C4 of rat of asthenia of the spleen, and The leaf of Panicled Fameflower on determinating mininal inhibitory concentration, swelling of mouse ear, capillary vein permeability of mouse skin, granuloma in rat.

①药效学实验:观察土人参根、叶对小鼠游泳时间的影响,以及对&脾虚&大鼠脾脏生理功能,细胞因子IL-2、IgA、IgM、IgG、C3、C4的影响,验证土人参根、叶&健脾&功效;观察土人参叶体外抗菌实验,对二甲苯致小鼠耳廓肿胀的影响,对小鼠皮肤毛细血管通透性增高的影响,以及对大鼠棉球肉芽肿的影响,验证土人参叶&解毒消痈&功效。

The method is duplicate sex of small rat blood-vessel is gawkish model, use Y- labyrinthian detect hemal sex is gawkish model, the improvement that observes leaf of different dosage ginkgo extracts content to learn memorial capacity to small rat action.

方法复制小鼠血管性痴呆模型,利用Y-迷宫检测血管性痴呆模型,观察不同剂量银杏叶提取物对小鼠学习记忆能力的改善功能。

A program was established for artificial rearing of germfree rat pups according to the law of rat pups' suck.

根据无菌大鼠乳鼠吮吸乳汁的规律,建立一套新的人工哺乳无菌乳鼠的方法。

Objective Observe the effect of total panax notoginseng on the proliferation and cell cycle of the SD rat's mesangial cells. To investigate the possible mechanism of tPNS's inhibiting effect in prevention and cure the glomerulitis and glomerular sclerosis of the MCs. Methods Extract the MCs from heathy SD rat, culture and passage the cells.

目的:观察三七总皂苷(total Panax Notoginseng Saponins, tPNS)对体外培养的SD大鼠肾小球系膜细胞(Mesangial cells, MCs)增殖以及细胞周期的影响,以探讨tPNS在防治肾小球炎症和硬化中的作用及可能机理。

Objective It is to observe the glossoscopy characteristic of gastric mucosal precancerous lesions rat.Methods Gastric mucosal precancerous lesions model was made with Wistar rat.The tongue nature,computer externalization analysis of tongue image,pH of tongue surface,tongue pathology and exfoliative cell of tongue coating were studied.

以Wistar大鼠造成胃黏膜癌前病变模型,从舌象肉眼观察、数码相机摄取舌像计算机客观化分析、舌面pH值检测、舌病理及舌苔脱落细胞观察等多方面进行研究。

AIM: To observe the effect of endothelin receptor antagonist (BQ123) on calcification in rat vascular smooth muscle cells in vitro. METHODS: Calcification of cultured rat VSMCs was produced by incubation with β-glycerophosphate.

目的:在离体钙化的血管平滑肌细胞上,观察内皮素受体阻断剂对血管平滑肌细胞钙化的影响,探讨内皮素促进细胞钙化的信号转导和分子机制。

Methods Both a rat model with habenular lesion and a hypnotic-treated rat model were established by passing a DC current or by infusing with zolpidem into the stomach and then the rats were divided randomly into habenular sham group,habenular lesion group,vehicle group and zolpidem group.The mRNA levels of adenosine and 5-HT-related genes were examined by RT-PCR in the PO/AH.

以30只雄性Wistar大鼠为实验动物,直流电损毁缰核或灌胃给予催眠药唑吡坦建立缰核损毁大鼠(n=15)和唑吡坦处理大鼠(n=15)两种动物模型,随后随机将大鼠分为缰核假手术组(n=7)、缰核损毁组(n=8)、生理盐水对照组(n=7)和唑吡坦处理组(n=8)。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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