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Results: It was found that the myocardial chymase-like activity increased markedly in hypertrophied rat (2,6,12 weeks);the angiotensinⅡand aldosterone contents increased significantly in hypertrophied rat (12 weeks);and the myocardium angiotensin Ⅱ,aldosterone levels correlated positively to the myocardium chymase- like activity .

结果:术后2、6、12周心肌肥厚组大鼠心肌组织糜酶样活性较同期正常对照组显著增高(P<0.01);术后12 周心肌肥厚组大鼠心肌血管紧张素Ⅱ、醛固酮含量较同期正常对照组明显增加(P<0.01);心肌组织血管紧张素Ⅱ、醛固酮水平与糜酶样活性呈正相关(P<0.05)。

There were many apoptotic cells spreading through the short cloacal membrane in cloaca malformation rat embryos on gestational days 13.5. But from gestational days 14.5 to 15 cellular apoptosis did not occur in terminal rectum and"tail groove" in cloaca malformation rat embryos, rectum could not connect to the outside.

ETU致畸的胎鼠13.5天时,短小的泄殖腔膜布满大量凋亡的细胞。14.5天-15天直肠末端与相应的尾沟处未见细胞凋亡发生,直肠末端未与外界相通。

Capsule of result antler glossy ganoderma can restrain the tumour of small rat of tumour of Lewis carry on one's shoulder to grow apparently, the elevatory, kidney of the Scr of serum of small rat of tumour of carry on one's shoulder that suitable platinum of remarkable changeover causes and BUN is coriaceous those who organize MDA content is elevatory, and SOD and GSH-Px reduce capsule of; antler glossy ganoderma actively poisonous to the cell of cell of tumour of different type person active has bigger difference, its IC50 sort is: HT-29

结果鹿角灵芝胶囊能明显抑制Lewis荷瘤小鼠的肿瘤生长,并显著逆转顺铂造成的荷瘤小鼠血清Scr和BUN的升高、肾皮质组织MDA含量的升高,以及SOD和GSH-Px活性的降低;鹿角灵芝胶囊对不同类型人肿瘤细胞的细胞毒活性有较大差异,其IC50排序为:HT-29

Right MCAO was induced in all animals by a 4-0 nylon thread with round tip inserted cranially into right internal carotid artery and maintained for 90 min. HSP70 mono-clone antibody immunocytochemistry was used to detect the changes of HSP70 expression in rat cortex at 6, 12,18,24,48 and 72h reperfusion in conjunction with histopathological analysis of rat brain.

应用HSP70单克隆抗体免疫组化染色检测大鼠再灌注6、12、18、24、48及72h脑皮质HSP70表达的变化;并与病理组织学改变进行对照研究。2.20只雄性新西兰大白兔随机分成对照组(n=10)及葛根素组(n=10),所有动物均阻闭腹主动脉肾下段20min,建立兔脊髓腰尾段缺血模型。

Methods Accelerated rat crescentic nephritis were induced by rabbit anti-rat GBM nephrotoxic serum.

目的 探讨CD44及其配体骨调素在实验性新月体肾炎发展过程中的表达和作用。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果:1合成的CS-PAA磁性聚合物微球呈球形,大小均一,TEM测定其干态下粒径为80nm左右,磁化曲线证实具有超顺磁性,具有一定的PH敏感性,固载表柔比星后,水溶液性状稳定,无沉淀物,DLS测定直径约200nm左右,测定载药率为15%,较文献报道高,包封率为20%。2肉眼观察试验组大鼠膀胱表面呈褐色,可见大量的Fe粒子聚集,普鲁士兰染色法显示,试验组大鼠膀胱壁内有大量的Fe粒子,分布至膀胱壁全层,与对照组大鼠相比,试验组大鼠的肝、脾内的Fe粒子聚集量明显降低;HPLC测定结果与Fe染色相同;高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高,其他组大鼠血生化指标没有明显变化。3MTT法发现,高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组,FCM发现试验药物组可引起明显的肿瘤细胞凋亡,试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C,试验组细胞Bcl-2蛋白减少,Bax蛋白变化不明显,Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P<0.01),其中高剂量组的抑制率最高。

Results Ligustrazine (20mg/kg,50mg/kg) obviously prolonged breath time of decollated mice and survival time of hypoxic mice induced by sodium nitrite; ligustrazine (10mg/kg,20mg/kg) markedly ameliorated rat neurological deficit, reduced infarct area in MCAO rat.

结果 川芎嗪20mg/kg、50mg/kg可明显延长断头小鼠的喘息时间及亚硝酸钠致死小鼠的生存时间;10mg/kg、20mg/kg可显著改善中动脉栓塞致脑缺血大鼠的行为障碍,降低脑缺血梗死面积。

Objectives A rat model sciatic nerve crush injury was reproduced in rat, and behavioral changes, pain threshold, thermal withdrawal latency and the changes in c-fos expression in cornu dorsale medullae spinalis were examined, and the relationship between peripheral nerve regeneration and hyperpathia was explored.

目的 建立大鼠坐骨神经挤压模型,观察其神经恢复过程中行为学、机械痛阈值、热缩足反射潜伏期以及脊髓背角c-fos表达(对c-fos表达产物:Fos蛋白阳性的神经元进行计数)的改变,探讨外周神经损伤再生与痛觉过敏的关系。

Methods:Special culture was performed in the fetal rat ependyma or adult rat bone marrow,respectively.

以细胞克隆及免疫细胞化学方法判断NSC增殖并鉴定NSC、神经元和神经胶质细胞。

Aim:To explore the feasibility of neural stem cells derived from both fetal rat ependyma and adult rat bone marrow.

目的 :探寻胚鼠室管膜和成鼠骨髓分离培养神经干细胞的可行性。方法:将分离的胚脑室管膜组织或成年骨髓细胞分别特殊培养。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力