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The method uses method of the PCR that cover type to undertake to viscera of small rat and shrewmouse head and small rat enlarge is added, detect LCMV gene; undertakes to serum ELISA experiments at the same time, have the result of two kinds of methods comparative.

方法采用套式PCR方法对小鼠和地鼠脑及小鼠脏器进行扩增,检测LCMV基因;同时对血清进行ELISA试验,将两种方法的结果进行比较。

Methods Cultivation of alveolar type Ⅱ cells were mode from normal adult rat and of silicotic animal model of rat was established.

分离培养正常成年大鼠肺泡Ⅱ型细胞,建立大鼠矽肺模型,用原位杂交和免疫组化技术检测体外培养的和矽肺病变中的肺泡Ⅱ型细胞TGFβ1和PDGF BmRNA和蛋白的表达。

Methods Cultivation of alveolar type Ⅱ cells were mode from normal adult rat and of silicotic animal model of rat was established. In situ hybridization and irnmunohistochemieal staining were applied to detect the gene expression of TGFβ1 and PDGF-B in the alveolar type Ⅱ cells of primary culture and silicotic tissue.

分离培养正常成年大鼠肺泡Ⅱ型细胞,建立大鼠矽肺模型,用原位杂交和免疫组化技术检测体外培养的和矽肺病变中的肺泡Ⅱ型细胞TGFβ1和PDGF-B mRNA和蛋白的表达。

Methods:Rat hepatocytes were isolatcd by a single two step perfusion method,placed in 96 hole tissue plates and incubated for 1.5 hours,then exposed to different concentrations of HGF for 48 hours and the proliferation of rat hepatocytes was determined by MTT colorimetric assay.

利用胶原酶原位二步灌注法分离大鼠肝细胞,接种并预培养 1 。5 h后,用含不同浓度 HGF的培养基继续培养至 48h,MTT比色法检测肝细胞生长因子对原代培养大鼠肝细胞增殖的影响。

When offering food during this time, you should stick to smaller items, like an adult mouse instead of a rat if your snake is a rat feeder.

这时候提供的食物规格应该小一些,例如大白鼠应该换成小白鼠。

The cross section area of desmin-positive fiber of soleus in glutamine-supplemented rat is larger than that in placebo-supplemented rat.

连续运动2天后,补充谷氨酰胺组的desmin阳性肌纤维比补充安慰剂组显著性增大。

The results showed that taurine has no significant influence on testis index, epididymis index, spermaduct index, penis index and testis tectology of baby and young rats, but can make old rat testis tectology better, prevent convoluted seminiferous tubule degeneration, germ cell distortion and leydig cell atrophy. Taurine increase the level of T, E2 and LH in baby rat obviously, but has no significant effect on the level of FSH. The level of T and LH was obviously increased by taurine in young and old rats, but the level of E2 and FSH has no significant changes.

结果证明:牛磺酸对幼年、青年、老年大鼠睾丸指数、附睾指数、输精管指数和阴茎指数没有明显影响;对幼年、青年大鼠睾丸组织形态没有影响,但可明显改善老年大鼠睾丸组织形态,阻止曲细精管退化、生殖细胞变形和间质细胞萎缩;显著促进幼年大鼠 T 、 E2、 LH 分泌,而对 FSH 没有明显影响;明显促进青年和老年大鼠 T 和 LH 分泌,而对 E2和 FSH 没有明显影响。

Methods: Rat MSC was isolated and purified in vitro,labelled by Hoechst33342, stereotaxis imbed into CA4 of rat hippocampus.

近来研究发现,MSC可以分化为神经元样细胞、胶质样细胞等[1],植入体内后对脑和脊髓的损伤性疾病有治疗作用[2],为MSC替代治疗神经系统疾病提供了实验基础。

Sprague Dawley rat models of caudate nucleus hemorrhage were established. 10 μL of cell suspension was separately collected from the OB-OEC transplantation group and OM-OEC transplantation group, injected into rat brain tissues (1 μL/min) using a microinjector under the stereotaxis. The same volume of medium was infused into the right caudate nucleus from rats of the control group.

取SD大鼠制备尾状核出血模型,嗅球来源嗅鞘细胞移植组及嗅黏膜嗅鞘细胞移植组各取10μL细胞悬液在立体定向下引导微量注射器向大鼠脑组织内匀速注射(1 μL/min),对照组注入等量培养基,注射点为右侧尾状核。

METHODS: TAFI was activated by thrombin and throbomodulin, and the conversion of TAFI to TAFIa was detected by chromogenic assay. A rat lung fibrin deposition model was induced by batroxobin. The effect of elevated levels of TAFIa on endogenous fibrinolysis was studied in the rat model.

①用凝血酶及血栓调节蛋白活化TAFI,然后用TAFI活性测定试剂盒检测活化后的TAFI;②以[125I]-纤维蛋白原进行示踪,用batroxobin建立大鼠肺纤维蛋白沉积模型,并运用该模型研究TAFIa对大鼠内源性纤溶的作用。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力