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Objective To investigate the effect of gossypol acetate on reproductive systems of male mice, providing useful data for development of environmentfriendly rat poison.

目的 探索醋酸棉酚对雄鼠生殖系统的影响,为生态灭鼠药的研制提供实验依据。

Neutrophils from normal animals were isolated from whole blood and incubated with plasma from rat gracilis muscle flaps on coverslips pretreated with ICAM.

嗜中性粒细胞从正常动物的全血中分离且用ICAM预处理过的盖玻片覆盖来自于大鼠股薄肌皮瓣的血浆孵化。

Content of result grackle extraction can reduce liver to injure the serum ALT of small rat apparently, AST and content of MDA of oar of liver divide evenly, XOD of oar of liver divide evenly is active, raise GSH active, liver organizes pathology to change to be reduced apparently.

结果白头翁提取物能明显降低肝损伤小鼠的血清ALT,AST和肝匀浆MDA含量,肝匀浆XOD活性,提高GSH活性,肝组织病理变化明显减轻。

The method uses CCl4 revulsive small rat liver injures a model, medicaments is preventive experiment, through determining transaminase of serum cereal third , millet straw and liver organization third 2 aldehyde content, yellow purine oxidizes peptide of pleasant of Guang of enzymatic, cereal is reductive enzymatic active and liver tectology change are index, observation grackle extracts the protective effect that content injures to liver.

方法采用CCl4诱导小鼠肝损伤模型,药物预防性实验,通过测定血清谷丙转氨酶、谷草转氨酶和肝组织丙二醛含量、黄嘌呤氧化酶、谷胱甘肽还原酶活性以及肝组织形态学变化为指标,观察白头翁提取物对肝损伤的保护功能。

METHODS: Rat BMSCs were isolated by density gradient method and cultured in 3 different concentrations of oxygen.

采用密度梯度法分离培养大鼠骨髓间充质干细胞,在3种氧浓度中进行培养。

PubMed abstract: Gramicidin perforated-patch-clamp recordings in brain slices were used to obtain an accurate assessment of the developmental change in the GABAA receptor reversal potential in embryonic and early postnatal rat neocortical cells including neuroepithelial precursor cells, cortical plate neurons, and postnatal neocortical neurons.

PubMed摘要:大脑部分的短杆菌肽穿孔片钳记录,用於获得胚胎中GABBA受体逆向电压发展改变的精确估计,以及出生后大鼠早期新生皮层细胞,包括神经上皮先驱细胞、皮层板神经元和出生后新皮层神经元的估计。

Yes; but this time it's more serious,' said the Rat gravely.

"不错,可这回问题更严重,"河鼠沉重地说。

MethodsThe rat single-pass intestinal perfusion technique was applied, and the gravimetry was used to correct the perfusion volume.

方法采用大鼠在体肠单向灌流吸

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因,并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列,通过对克隆的降钙素序列的比较研究,结果显示同一科的鱼降钙素序列保守性较高,同时,根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究,结果显示在分类学上,分类地位较远的鱼,其降钙素相似性较低,分类地位越接近的鱼,其降钙素相似性越高;我们利用谷胱甘肽S-转移酶(Glutathions S-transferase,GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究,应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索,并将纯化的融合蛋白作为抗原,获得了高效价的兔抗鲑鱼降钙素免疫血清;生物活性研究表明,大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Well, when I see rat poison, I grind it up and add it to my coffee to build up my strength.

我嘛,我看见老鼠药时,就把它嚼成碎末,然后加进我的咖啡里,以此来增加气力。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力