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Objective To investigate which stage is the most important and the relationship with kupffer cell,during isoflurane decrease anoxia-reoxygenation injury in isolated rat liver.Methods We established the model of isolated rat liver.

目的 明确异氟醚减少离体鼠肝缺氧-复氧损伤的作用主要发生在哪一阶段,以及与枯否细胞的关系。

Objective To investigate which stage is the most important and the relationship with kupffer cell,during isoflurane decrease anoxia-reoxygenation injury in isolated rat liver.Methods We established the model of isolated rat liver.

udw,dNR 目的明确异氟醚减少离体鼠肝缺氧-复氧损伤的作用主要发生在哪一阶段,以及与枯否细胞的关系。

The distribution of the growth-associated protein (GAP-43) which is close related to the axon regeneration was examined by immunocytochemical method in the adult rat brain and the rat parietal lobus injuried into which the fetal cerebral suspension was lransplanted.

在发现流式细胞计可用于神经科学的研究后,用FCM研究了大鼠脑发育过程中细胞周期的变化和胚胎大脑细胞移植到成年大鼠顶叶后对宿主顶叶脑细胞分裂的影响;由于生长相关蛋白(GAP-43)与轴突再生密切相关,用免疫细胞化学法研究了GAP-43在成年大鼠脑内的分布及顶叶损伤植入胚胎大脑细胞后GAP-43的变化。

Results TAEP can significantly decrease the contents of monoamine neurotransmitter in the straite body and the bordering area of rat forebrain, increase the contents of neutral and acidic metabolites in the straite body, and increase the contents of 5-HIAA and HVA of the monoamines in the bordering area of rat forebrain.

结果 TAEP对大鼠纹状体的单胺类神经递质的含量具有显著的降低作用,同时升高纹状体单胺类递质中性及酸性代谢产物的含量;对大鼠前脑边缘区的单胺类递质具有显著的降低作用,同时显著升高边缘区单胺代谢物5—羟吲哚乙酸(5—HIAA),高香草酸的含量。

Aim:To study the experimental methodology based on cerebral microdialysisand high performance liquid chromatography with electrochemicaldetection, make the acute rat model of Parkinsons diseasesinduced by 6-hydroxydopamine(6-OHDA), examine the effects of the generalcistanosides on the striatal extracellular levels of monoamines neurotransmittersin rat model of PD and find the possible mechanisms of the neuroprotective actionproduced by GCs.

目的:建立基于脑微透析和高效液相-电化学检测法的实验方法;制作6-羟基多巴胺(6-OHDA)损伤致急性帕金森病大鼠模型;研究肉苁蓉总苷对6-OHDA损伤致急性PD模型大鼠纹状体细胞外液中单胺类递质含量的影响,以探讨其对脑神经保护作用的可能机制。

Adoping the simple and easy anthology to gather the unit to gather urine of 5 hours measures, Watch different series of urine quantitative changeization .(2) Experiment of immunity: Adopted CY methods copy immunity to repress the little rat pattern, To divide randomly small healthy white mice originated from kunming into normal contrast group ,pattern group, Fulin group, Guizhi group,Fulin and Guizhi group (Two medicine are according to 1 : 1、2: 1、 1: 2、 4: 3 the proportion compose ).Be total to 8 groups . To observed Fulin and Guizhi different combinition adjust the repressing small mice's spleen and thymus, Ascertaining by measuring little rat phagocyte the gobbleing uprate and serum IL-2 -* TNF — a 's content.

2免疫实验采用环磷酰胺法复制免疫抑制小鼠模型,健康昆明种小鼠随机分为正常对组、模型组、茯苓组、桂枝组、茯苓与桂枝配伍组(两药按1:1、2:1、1:2、4:3比例进行组合)共8组,观察茯苓桂枝药对及其各配伍组对免疫抑制小鼠脾、胸腺的影响,测定小鼠吞噬细胞吞噬率及血清IL-2、TNF—α含量。

The result showed that chromatogram peaks were summated,peak area increased and two new peaks were found.It suggested that the composition dissolution of RP was increased with the addition of RAT,chemical changes may be occurred after compatibility. Established the HPLC analysis method forα-asarone in RAT and researched the content change ofα-asarone after compatibility.

发现配伍后色谱峰具有加和性,峰面积增加,有两个新峰产生,提示石菖蒲能使远志中的化学成分溶出度增加,两药配伍后可能发生了复杂的化学变化;建立石菖蒲中α-细辛醚的HPLC分析方法,研究配伍远志后α-细辛醚的含量变化。

Fulwiler CE, Saper CP. Subnuclear organization of the efferent connections of the parabrachial nucleus in the rat. Brian Res 1984;319:229-259.[2]Herbert H, Moga MM, Saper CB. Connections of the parabrachial nucleus with the nucleus of the solitary tract and the medullary reticular formation in the rat. J Comp Neurol 1990;293:540-580.[3]Slugg RM, Light R.

三叉神经脊束间质核(interstitial nucleus of the spinal trigeminal tract, INV)为位于延髓闩平面附近三叉神经脊束内及其周围的一些核团,由中、小型梭形、圆形或卵圆形细胞组成,经三叉神经的分支和迷走、舌咽神经接受外周内脏和躯体感觉信息[6,7]。

In order to explore application of collagen from rat rail tendon to experimental medicines,the collagen,extracted from solvating fresh rat rail tendons by using a simple economical method,has been prepared collagen gel.

周志瑜 ,廖小宜,余海为了探索鼠尾肌腱胶原蛋白在实验医学中的应用,用简单的方法溶解新鲜大白鼠鼠尾肌腱,从中提取较纯的胶原蛋白,制成胶原凝胶。

Then to improre the change tendency of Senile rat's bone metabolism.Conclusion Intaking high does of D-galactose for a long time can cause Wistar male rat aging and induce the changes of sclerotin meanwhile.But zeaxanthin can delay the occurrence of the changes.

结论长期大量摄入D-半乳糖导致大鼠衰老的同时可能诱发其发生骨质衰老变化,而玉米黄质可以延缓此类致老性骨质变化的发生。

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