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c-met proto-oncogene may play a role in the development of rat pancreas, especially the islet remodification of postnatal rat pancrcatic development.

提示c-met可能在胰腺发育过程中起到调控作用,参与胰腺发育中新生后胰岛结构重塑过程。

The results that the salic acid content of rat's serum in pVHN group was far lower than that of control group (P.01) showed that the pVHN GNA could reduce the salic acid content of rat's serum.

大鼠血清唾液酸含量测定结果显示, pVHN组动物血清唾液酸含量明显低於荷瘤对照组,与荷瘤对照组比较差异极显著(P.0l ),证明HN基因对於去除血清唾液酸具有明显的作用。

In recent years, NAFLD animal models have been successfully established in many species such as rat, sand rat, mice, rabbit, and piglets induced by diet, total parenteral nutrition, and methionine choline-deficient. These models provide new opportunities to study the pathogenesis and treatment of metabolic syndrome associated with NAFLD/NASH.

近年来,在大鼠、沙鼠、小鼠、兔和小猪等动物种属成功地建立了食物、胃肠外营养与蛋氨酸胆碱缺乏等诱导的单纯性脂肪肝和脂肪性肝炎动物模型,这些模型为研究脂肪肝和脂肪性肝炎的发病机理与治疗提供了机会。

In recent years, NAFLD animal models have been successfully established in many species such as rat, sand rat, mice, rabbit, and piglets induced by diet, total parenteral nutrition, and methionine choline-deficient.

近年来,在大鼠、沙鼠、小鼠、兔和小猪等动物种属成功地建立了食物、胃肠外营养与蛋氨酸胆碱缺乏等诱导的单纯性脂肪肝和脂肪性肝炎动物模型,这些模型为研究脂肪肝和脂肪性肝炎的发病机理与治疗提供了机会。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明:①在适宜的浓度体外培养条件下,NSCs能形成典型的神经干细胞克隆球,Vimentin免疫荧光染色阳性,单靠丝裂原刺激或NSCs自我调节和分化诱导,不会产生典型的终末分化的成熟神经细胞;②海马源性的NSCs维持干细胞特性的时间更长,生物学特性更优;③传至第2~3代的NSCs尚未分化时移植最佳;④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞;⑤各种子代克隆球和细胞存在广泛的突触联系;⑥NSCs与OECs联合培养时,不需丝裂原刺激即能形成克隆球,获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞;⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化,但并不出现终末分化;⑧本研究培养条件不利于成纤维细胞、O4生长。

The method uses method of the PCR that cover type to undertake to viscera of small rat and shrewmouse head and small rat enlarge is added, detect LCMV gene; undertakes to serum ELISA experiments at the same time, have the result of two kinds of methods comparative.

方法采用套式PCR方法对小鼠和地鼠脑及小鼠脏器进行扩增,检测LCMV基因;同时对血清进行ELISA试验,将两种方法的结果进行比较。

Methods Cultivation of alveolar type Ⅱ cells were mode from normal adult rat and of silicotic animal model of rat was established.

分离培养正常成年大鼠肺泡Ⅱ型细胞,建立大鼠矽肺模型,用原位杂交和免疫组化技术检测体外培养的和矽肺病变中的肺泡Ⅱ型细胞TGFβ1和PDGF BmRNA和蛋白的表达。

Methods Cultivation of alveolar type Ⅱ cells were mode from normal adult rat and of silicotic animal model of rat was established. In situ hybridization and irnmunohistochemieal staining were applied to detect the gene expression of TGFβ1 and PDGF-B in the alveolar type Ⅱ cells of primary culture and silicotic tissue.

分离培养正常成年大鼠肺泡Ⅱ型细胞,建立大鼠矽肺模型,用原位杂交和免疫组化技术检测体外培养的和矽肺病变中的肺泡Ⅱ型细胞TGFβ1和PDGF-B mRNA和蛋白的表达。

Methods:Rat hepatocytes were isolatcd by a single two step perfusion method,placed in 96 hole tissue plates and incubated for 1.5 hours,then exposed to different concentrations of HGF for 48 hours and the proliferation of rat hepatocytes was determined by MTT colorimetric assay.

利用胶原酶原位二步灌注法分离大鼠肝细胞,接种并预培养 1 。5 h后,用含不同浓度 HGF的培养基继续培养至 48h,MTT比色法检测肝细胞生长因子对原代培养大鼠肝细胞增殖的影响。

When offering food during this time, you should stick to smaller items, like an adult mouse instead of a rat if your snake is a rat feeder.

这时候提供的食物规格应该小一些,例如大白鼠应该换成小白鼠。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力