查询词典 rat-face

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

Results Ligustrazine (20mg/kg,50mg/kg) obviously prolonged breath time of decollated mice and survival time of hypoxic mice induced by sodium nitrite; ligustrazine (10mg/kg,20mg/kg) markedly ameliorated rat neurological deficit, reduced infarct area in MCAO rat.

结果 川芎嗪20mg/kg、50mg/kg可明显延长断头小鼠的喘息时间及亚硝酸钠致死小鼠的生存时间；10mg/kg、20mg/kg可显著改善中动脉栓塞致脑缺血大鼠的行为障碍，降低脑缺血梗死面积。

Objectives A rat model sciatic nerve crush injury was reproduced in rat, and behavioral changes, pain threshold, thermal withdrawal latency and the changes in c-fos expression in cornu dorsale medullae spinalis were examined, and the relationship between peripheral nerve regeneration and hyperpathia was explored.

目的 建立大鼠坐骨神经挤压模型，观察其神经恢复过程中行为学、机械痛阈值、热缩足反射潜伏期以及脊髓背角c-fos表达（对c-fos表达产物：Fos蛋白阳性的神经元进行计数）的改变，探讨外周神经损伤再生与痛觉过敏的关系。

Methods:Special culture was performed in the fetal rat ependyma or adult rat bone marrow,respectively.

以细胞克隆及免疫细胞化学方法判断NSC增殖并鉴定NSC、神经元和神经胶质细胞。

Aim:To explore the feasibility of neural stem cells derived from both fetal rat ependyma and adult rat bone marrow.

目的 ：探寻胚鼠室管膜和成鼠骨髓分离培养神经干细胞的可行性。方法：将分离的胚脑室管膜组织或成年骨髓细胞分别特殊培养。

Experimental study on culture and identification of neural stem cells from both ependyma of fetal rat and bone marrow of adult rat［J］.

胚鼠室管膜及成鼠骨髓神经干细胞分离培养及分化鉴定的实验研究［J］。

INTERVENTIONS: Embryonal 14-day SD retina was transplantedin-between the epithalamus and hypothalamus of the midbrain in P1 rat and the right eye of the P1 rat was removed simultaneously. The development time of TP on the 9th day after operation was corresponding to the normal retina on the 1st day after born, which was then recorded as TP1, and so on. The development and differentiation was shown by histochemical method.

干预：用胚14 d SD大鼠视网膜移植至P1大鼠中脑左侧上、下丘间，同时摘除P1鼠右眼，术后第9天移植视网膜的发育时间与生后第1天正常视网膜相当，记为TP1，余类推，应用组织化学方法显示神经元的发育和分化。

To investigate the role of gene Gli3 in idiopathic congenital talipes equinovarus, we constructed the Gli3 luciferase reporter gene expression vectors to analyze the promoter activity of the rat gene Gli3. The regulatory element in the promoter region of the rat Gli3 was predicted using P-Match software and further verified by ChIP experiment.

为了探讨人类GLI3基因在单纯性马蹄内翻足(Idiopathic congenital talipes equinovarus， ICTEV)发生时所起的作用，文章构建了大鼠Gli3基因启动子区域荧光素酶报告基因表达载体来分析Gli3基因启动子的活性，并利用P-Match软件预测大鼠Gli3基因启动子区域可能的调控元件，应用ChIP实验加以验证。

Methods:①Pharmacodynamics observed the effects of The root and leaf of Panicled Fameflower on swimming time of mouse, and physiological function, IL-2 IgA IgM IgG C3 C4 of rat of asthenia of the spleen, and The leaf of Panicled Fameflower on determinating mininal inhibitory concentration, swelling of mouse ear, capillary vein permeability of mouse skin, granuloma in rat.

①药效学实验：观察土人参根、叶对小鼠游泳时间的影响，以及对&脾虚&大鼠脾脏生理功能，细胞因子IL-2、IgA、IgM、IgG、C3、C4的影响，验证土人参根、叶&健脾&功效；观察土人参叶体外抗菌实验，对二甲苯致小鼠耳廓肿胀的影响，对小鼠皮肤毛细血管通透性增高的影响，以及对大鼠棉球肉芽肿的影响，验证土人参叶&解毒消痈&功效。

The method is duplicate sex of small rat blood-vessel is gawkish model, use Y- labyrinthian detect hemal sex is gawkish model, the improvement that observes leaf of different dosage ginkgo extracts content to learn memorial capacity to small rat action.

方法复制小鼠血管性痴呆模型，利用Y-迷宫检测血管性痴呆模型，观察不同剂量银杏叶提取物对小鼠学习记忆能力的改善功能。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。