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Methods To create the rat model of gastric ulcer cauterized by acetum. The rat we treated by drinking alkalescence ion water which pH value we difference and to observe the state of rat gastric ulcer haling.

采用醋酸烧灼型大鼠胃溃疡模型,对整水器处理后生产的不同pH值的碱性离子水对胃溃疡愈合的作用进行研究。

Objective It was reported that rALR (rat augmenter of liver regeneration) distribute in most of tissue and organ of rat, no-tissue specility; mRNA expression of rALR was identified variedly in different tissue, strongest in testis. But nowadays there was not some study on ALR in rat testis. Here to confirm the expression and localization of ALR in infancy,adult and old rat testis,comparing the change of ALR expression degree before per 70 PH and after per 70 PH. In order to evaluate expression principle of ALR in rat testis and know if ALR has tight connection with spermatogenesis and development of testis, to explore the relation between ALR and reproduction, to discover the possible expression character and functional methods of ALR.

目的:文献报道rALR分布于各组织器官,无组织特异性,但其mRNA在各组织的表达强度有差异,在睾丸组织表达最强,目前对大鼠睾丸ALR表达尚无深入的研究;本实验研究不同年龄段大鼠睾丸组织中肝再生增强因子的表达水平、表达部位、以及表达的方式,进而探讨睾丸ALR与睾丸发育、精子生成以及生殖活动的关系;同时探究睾丸组织ALR表达是否参与了肝脏的再生过程中,进一步了解睾丸ALR表达的特性及作用方式。

The results show that: This tablet can decrease the rat"s footpad swell induced by Freud"s, inhibit the permeability of the blood capillary in inflammatory joint and decrease the 11-2 level in serum while increasing the rat"s body weight,(2) It can lower the rat"s temperature and the permeability of the blood capillary in inflammatory joint induced by collagen and inhibit the delayed type hypersensitivity,It can lower the stickiness of full blood and the accumulation of erythrocyte obviously inblood stasis model rats,lt can inhibit the formation of hemolysin and decrease the index of carbon granule clearance in mice, It can lower the permeability of celiac blood capillary and inhibit the granulomatous hyperplasia by sc cotton ,It can inhibit the pain induced by acetic acid and raise the pain threshold value of heat stimulation.

结果表明:关络通片能明显降低佐剂性关节炎大鼠左右足趾肿胀度,降低大鼠炎症关节的通透性,抑制其IL-2含量,对大鼠体重增长缓慢有一定的改善作用;能改善Ⅱ型胶原诱导性关节炎大鼠的表征指标及炎症关节的通透性,对其迟发型超敏反应有一定的抑制作用;对血瘀模型大鼠,能显著性降低其全血粘度、血浆粘度、红细胞压积;能抑制小鼠的溶血素抗体生成,抑制小鼠网状内皮系统的吞噬能力,具有一定的抑制机体免疫功能的作用,能抑制小鼠腹腔毛细血管通透性增高,抑制大鼠棉球肉芽组织增生,具有一定的抗炎作用;能抑制小鼠醋酸扭体反应、提高小鼠热板痛阈值,具有一定的镇痛作用。

The study of mechanism of its anti-inflammationThis experiment shows that NTL can inhibit the edema of hind paw induced by fresh egg white in adrenalectomized rat, reduce the weight of thymus in rat, increase the content of hepatic glycogen ,but decrease eosinophil of mice through experimentalstudy of effect of NTL on edema of hind paw induced by fresh eggwhite in adrenalectomized rat, weight of thymus in rat, content ofhepatic glycogen and eosinophil of mice. The results indicate that NTL has the analogous function to cortin.

抗炎机理研究通过虎斑游蛇对去肾上腺大鼠蛋清性足跖肿胀、对大鼠胸腺重量和对小鼠肝糖原含量及对小鼠嗜酸性粒细胞数量影响的实验研究,表明虎斑游蛇能够抑制去肾上腺大鼠蛋清性足跖肿胀,使小鼠胸腺的重量减轻,增加小鼠肝糖原的含量而减少嗜酸性粒细胞的数量,提示虎斑游蛇具有肾上腺皮质激素样作用。

On the basis, further isolated-living condition and moderate unpredictable stress are applied successively to set up PSD rat model . The neurological functional recovery of stroke rat is assessed by Longa 5 grades and horizontal bar test.Sucrose solution consumption test, open-field test and passive avoid test are used to estimate the change of interest, the level of spontaneous motor activities and investigative behavior of PSD rat model.Fluorophotometry is applied to detect the monoamine neurotransmitters(NE,5-HT and DA) of the double frontal-parietal cortexes and brain stem. Investigating the action of YuLeShu on the neurological functional recovery of stroke rat.

运用Longa5法和水平木棒法评定卒中大鼠神经功能恢复;运用糖水实验、敞箱实验、被动躲避实验评定PSD大鼠的兴趣感变化和自发活动、探究行为等抑郁行为学改变;采用荧光分光度法测定双侧额顶皮层及脑干的单胺递质:去甲肾上腺素(Norepinephrine,NE)、5-羟色胺(5-Hydroxytryptamine,5-HT)、多巴胺(Dopamine,DA)含量。

The present invention selects and uses hereditary hypertension rat and rat with normal blood pressure to make hybridization, and the rat whose blood pressure is normal and arterial pressure susceptivity reflection sensitivity is less than 0.3 ms/mm Hg can be screened out from filial generation, bred and propagated, and repeatedly bred and propagated, after above 12 generations so as to obtain the invented pure rat with stable hereditary property and spontaneous arterial pressure susceptivity reflection functional defect.

本发明涉及医学实验动物模型技术领域,是培育用于心血管疾病防治研究的一种自发性动脉压力感受性反射功能缺陷的纯种大鼠的方法。本发明选用遗传性高血压大鼠和正常血压大鼠杂交,从子代中筛选出血压正常、动脉压力感受性反射敏感性低于0.3ms/mmHg的大鼠培育繁殖,并反复按此标准在其下一代中筛选、繁殖,经12代以上便得到遗传性稳定的自发性动脉压力感受性反射功能缺陷纯种大鼠。

Use biochemical method to observe the effect of electro-acupuncture on AchE、SOD、MAO、NO、NOS of senile dementia AD rat's brain tissue; use immunohistochemistry technique to observe protein kinase expression of hippocampal; use optical and electron microscope to observe morphosis change of rat's brain tissue; use in situ hybridism technique to observe aspartate receptor mRNA expression of hippocampal; use water and electric maze experiment to observe rat escape latent period and mistake times, to understand it's learning and memory ability.

分别用生化方法观察电针对AD大鼠脑组织胆碱酯酶、超氧化物歧化酶、单胺氧化酶、一氧化氮、一氧化氮合酶的影响;用免疫组化方法观察海马组织蛋白激酶的表达;用光、电镜方法观察大鼠脑组织形态结构的变化;用原位杂交方法观察海马组织天门冬氨酸受体rnRNA的表达;用水迷宫和电迷宫实验观察大鼠逃避潜伏期和错误次数,以了解其学习记忆能力。

ResultsComparing with gonadectomy Shenyangxu model group, Wenyangyishen Capsules can improve upset excitability of male rat of gonadectomy penis Home News for Overseas Service Department , shorten erection incubation period; The rat increasing gonadectomy male sex attaches weight modulus of sexual organ; Can urge the hypophysis , adrenal gland weight modulus to increase by, weight adjusting the thymus gland , spleen; Elevate rat serum testosterone、Cortisol contents obviously; Be caused by messenger Cortisol the various Shenyangxu model small rat symptom gets laxation disappear even.

结果 与去势肾阳虚模型组比较,温阳益肾胶囊能提高去势雄性大鼠阴茎对外部刺激的兴奋性,缩短勃起潜伏期;增加去势雄性大鼠附性器官的重量系数;能促使垂体、肾上腺重量系数增加,调节胸腺、脾脏的重量;明显升高大鼠血清睾酮、皮质醇含量;使氢化可的松所致阳虚小鼠各项症状得到缓解甚至消失。结论温阳益肾胶囊具有益肾壮阳的功效。

Methods The rats were allocated to isogene group (n = 24, liver grafts from Lewis rat to Lewis rat) or allogene group (n =24, liver grafts from DA rat to Lewis rat).

实验分两组,同基因组,Lewis-Lewis间24只;异基因组,DA-Lewis间24例。

The animal model of this method system have, the characteristics of the high fat, high leptin, high Ins, and match with the characteristic of clinical and the pathologic of simple obesity. This studies showed: The acupuncture can obviously reduce the vaule of intaking food and water of the fat rats, and lower the body weight and fat; The acupuncture can urge the diameter, area, physical volume of white fat cell contract, Brown fat tissue of fat rat group surroundings vascular containing large quantity of white fat cell, the acupuncture group was less. this showed: The acupuncture enhanced the white fat cell metabolism, and promotesed the white fat cell to convert to brown fat cell; The frequency of spontaneous electric of nerve cell of pvn of the fat rat is high, the acupuncture counld depress the spontaneous electric of nerve cell of pvn, This showed: the acupuncture can depress the appetite passing to adjust function of pvn nerve; The monoaminergic neurotransmitter of inside of the fat rat brain is disfunction, the acupuncture can rectify this disfunction, and adjust level of 5-HT, and affect appetite, and improve metabolism; The level of leptin and Ins of fat rat increased high, The level of it of acupuncture group decreased, compared with the fat group p.001, This showed: the fat rats have the defeat of leptin and Ins in the body, The acupuncture can lowering this defeat, The level of leptin and Ins of the fat rat in the brain is less than the normal group, The level of leptin of the acupuncture group is high than the fat group, This showed: the acupuncture can increase the transportation of leptin from blood to brain.

本方法所制肥胖大鼠模型具有高体重、高脂、高leptin和高Ins血症的特点,符合人类单纯性肥胖病的临床和病理特征;实验研究显示:针灸能明显减少肥胖大鼠的摄水、摄食量及降低其体重、体脂;针灸能促使白色脂肪细胞直径、面积、体积缩小,减少肥胖大鼠棕色脂肪组织血管周围白色脂肪细胞数量,显示:针灸增强了白色脂肪细胞的代谢,促进了白色脂肪细胞向棕色脂肪细胞的转化:肥胖大鼠PVN神经细胞自发放电频率增加,脑内单胺递质紊乱,针灸通过抑制PVN亢奋的自发放电,调整5-HT水平,达到影响食欲,改善代谢的作用;肥胖大鼠血中leptin及Ins水平增高,与正常组大鼠比有明显差异,提示大鼠体内存在leptin及Ins抵抗,针灸后肥胖大鼠随着体重体脂的下降,血中leptin、Ins水平降低,与肥胖组比均有显著性差异,而针灸后大鼠脑内leptin增加,与肥胖组比P.05,提示:针灸具有改善leptin、Ins抵抗及增加leptin脑内转运的作用。

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