查询词典 rat gall

Methods Injecting quinolinic acid into rat hippocampus after injecting D-galactose to form the dementia rat model.

在s.c.D-半乳糖造成大鼠亚急性衰老的基础上，双侧海马内注射喹啉酸造成类AD模型。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

The resalt shows the presence of ANFm RNA in rat nasal mucosa, and reveals that ANF may be synthesized in rat nasal mucosa.

鼻腔粘膜中ANF mRNA与脑组织中的ANF mRNA含量相似。

On the basis of establishing an rat model, the aim of this study was to investigate the effect of castor oil-diet and/or ricinolic acid,the active component of castor oil on the synthesis of PGE_2, on the expression of COX-2 gene (clycloxygenase-2) and on the cervical ripening in pregnant rat.

本研究在建立引产餐妊娠大鼠引产模型的基础上，探讨蓖麻油引产餐和蓖麻油中活性成份一蓖麻酸对妊娠大鼠体内组织前列腺素(PGE_2)合成对前列腺素合成酶环氧化酶-COX2。

The biological characteristics and toxicity of Russula subnigricans Hongo was studied for the first time from ecology and morphologic characteristics and histology, the orthogonal experiment of the optimum culture condition, the analysis of components, apoptosis of the cells from little white rat liver and kidney induced by extract of Russula Subnigricans Hongo,to the histopathologic changes observation of little white rat liver and kidney through ecological observation, light microscopy and scanning electron microscopy, reversed-phase high performance liquid chromatography, agarose gel electrophoresis, transmission electron micioscopy.

利用菌种分离技术、光镜技术、电镜技术、高效液相色谱技术、毒理实验技术、电泳方法等对亚稀褶黑菇的生物学特性和毒性机理进行了研究，主要包括以下内容：亚稀褶黑菇的生态学和组织学研究、菌种分离培养、扫描电镜观察、成分分析、粗毒液诱导小自鼠肝肾细胞凋亡，小白鼠中毒后肝肾细胞透射电镜观察，研究结果如下： 1。

The apoptosis induced by extract of Russula subnigricans Hongo was investigated in little white rat liver and kidney cells by agarose gel electrophoresis.The result showed that agarose gel electrophoresis of DNA extracted from poisoned little white rat liver and kidney cellsrevealed typical 180 ~ 200bp integer-fold "ladder" bands.

亚稀褶黑菇提取液能诱导小白鼠肝肾细胞凋亡，亚稀褶黑菇提取液诱导肝肾细胞凋亡是亚稀褶黑菇中毒后机体病变死亡的重要表现形式之一 5。

In this study, we implement and investigate the performance of a more advanced row-action maximum likelihood algorithm and compare its performance to OSEM and MLEM on an Inveon microPET for small animals. We used a clinical rat PET study acquired and reconstructed the rat sinogram using MLEM, OSEM, RAMLA.

本研究针对Inveon microPET探讨列运算最大相似度演算法之效能和可行性，也与最大相似度与均值最大化演算法及序列子集均值与最佳化演算法比较。

Reconstruct ion of interstitial collagen in sinuatrial node occur since the rat diabetes has been duplicated for 16 weeks. The unbalance between collagen type Ⅰ and type Ⅲ in sinuatrial node of diabetes rat can be reversed by insulin treatment.

Ⅱ型糖尿病大鼠窦房结16周后出现间质胶原重构，胶原类型比例失衡；Ⅱ型糖尿病大鼠行胰岛素降低血糖后可不同程度下调窦房结间质的沉积，一定程度上逆转胶原类型比例紊乱。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养，用流式细胞仪检测细胞的免疫表型。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

But this is impossible, as long as it is engaging in a market economy, there are risks in any operation.

但是，这是不可能的，只要是搞市场经济，是有风险的任何行动。

We're on the same wavelength.

我们是同道中人。