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Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样,经微球菌溶解实验证明,3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L;从每组小鼠的12种组织提取总RNA,经Dot blotting检测证明,三种重组载体除在乳腺中表达外,p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达;在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

PCSS-driven Pockels cells: the PCSS delivers a sub-nanosecond pulse to an electro-optical crystal which controls the lasing efficiency in laser cavities ranging from microchip microjoule lasers to table-top multiJoule lasers.

PCSS 驱动的 Pockels 细胞: PCSS 递送子十亿分之一秒脉膊给一个电镀物品-光学的在激光中控制激光法效率的水晶洞排范围从微集成电路 microjoule 激光到桌子-顶端的多焦耳激光。

Misconfigured websites might misidentify a new version of IE 7 as an outdated version, thus returning web pages with results ranging from rendering and behavior issues to total rejection or denied of access to the user.

错误的网站可能misidentify一个新的IE 7版本是一种过时的版本,因此,返回网页结果不等,从渲染和行为问题,完全拒绝或否认的访问给用户。

This strain is able to degrade polycyclic aromatics such as naphthalene and biphenyl as well as monocyclic aromatics such as phenol. Under experimental conditions, strain DF51 is capable of degrading at concentrations of phenol ranging from 100 mg to 800 mg, which is catabolized mainly through the catechol 1, 2-dioxygenase pathway with a minor catechol 2, 3-dioxygenase pathway.

DF51。在本实验条件下,菌株DF51能够有效降解浓度范围为100-800mg L-1的苯酚,该菌代谢苯酚主要是通过邻苯二酚1, 2-双加氧酶催化开环途径进行,同时辅以邻苯二酚2, 3-双加氧酶催化开环,表明菌株DF51兼有混浊红球菌(Rhodoccocus opacus R7)和红球菌PNAN5Rhodoccocus sp。

The importance of the role of these morphogenic substances, as well as their mechanism of action, is evident by the fact that they have been conserved throughout evolution, where different variants can be found to exist in species ranging from worms to fruit flies and up to higher species including humans.

这些形态因子及其作用机理的重要性是显而易见的,事实上,在进化过程中这些因子是非常保守的,不同的物种包括蠕虫,果蝇以及高级物种人类在内,都存在形态因子的不同形式。

Excluded:(1) multilocular renal swelling Xiang: The renal premium ranging from anechoic area is too small to occupy the largest to 7-8cm.

排除:(1)肾多房襄肿:该肾补太小不等无回声区占据,最大到7-8cm。

Samples were subjected to high pressure ranging from 100 to 500 MPa. After high pressure treatments, the flavour compounds were extracted by Solid-Phase Microextraction. Analysis was carried out by Gas Chromatography-Mass Spectrometry. The key flavour compounds of orange juice were three alkenes (namely limonene, myrcene and α-pinene), two alcohols (linalool and α-terpineol), three aldehydes, one ketone and two esters (ethyl butyrate and 3-OH-ethyl hexanoate).

采用固相微萃取(Solid Phase Microextraction,SPME)方法对不同压力(100~500 MPa)超高压处理的鲜榨橙汁中的香气成分进行富集,并经气相色谱-质谱联用仪(Gas Chromatography-Mass Spectrometry,GC-MS)检测分析,橙汁中的主要香气成分有:3种烯(柠檬烯、月桂烯、α-蒎烯);2种醇;3种醛;1种酮;2种酯(丁酸乙酯、3-OH-己酸乙酯),它们是构成橙汁的主要特征致香成分。

Consumption of these compounds has been linked to the prevention of diseases ranging from cancer to neurodegenerative disorders.

食用这些化合物已联系到疾病的预防从癌症到神经退行性疾病。

Methods: A brain concussion was produced in rats using a simple pendulum device model. One control (n=12) and six experimental groups were used(n=12 in each group). The cerebral concussion groups were assigned to sacrifice times ranging from 1 day to 24days after injury.A Morris Water Maze memory paradigm was used to assess learning and memory function. Pycnosis degeneration or necrosis neurons in the locus cerebral cortex, dorsal hippocampus, dentate fornix and brainstem reticular formation were observed and counted after brain concussion. Cholinergic neurons in the basal forebrain and brainstem reticular formation were identified and quantitated using choline acetyltransferase.

应用自制单摆式闭合性机械打击装置建立脑震荡大鼠模型;成年SD大鼠随机分为1个对照组(n=12)和6个脑震荡组(即伤后1、2、4、8、16、24d,n=12);应用Morris水迷宫(Morris Water Maze,MWM)实验评价大鼠的学习记忆功能;用光镜对大鼠的上述脑区的固缩变性坏死细胞进行观察和计数;用免疫组化方法检测基底前脑和脑干网状结构的胆碱乙酰转移酶(cholin acetyltransferase,ChAT)阳性细胞的表达。

In 1618 she published a collection of thirty-six solo songs and soprano/bass duetsthat is a compendium of contemporary styles, ranging from intensely moving, harmonically adventurous laments to joyful sacred songs in Italian and Latin to witty strophic songs about the joys and perils of romantic love.

她在1618年出版了30集,6首歌曲,女高音独唱/低音二重奏(金正日普里莫图书出版社阿尔穆西切)这是一个现代风格的汇编,从激烈的运动,和声冒险感叹在欢乐的歌曲到意大利的神圣和拉丁美洲的机智有关的乐趣和浪漫爱情的危险strophic歌曲。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。