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For cloning antifreeze protein gene from a desert darkling beetle Microdera punctipenis dzunarica in Xinjiang, the primers were designed according to the core sequence of AFP gene deposited in GenBank, and the cDNA fragment about 294 bp named as MpAFP5 was amplified with the RT-PCR and 3'-RACE technique. Sequence analysis revealed that the cloned cDNA fragment named as MpAFP5 coded the mature peptide of AFP.

根据GenBank中已发表的昆虫抗冻蛋白基因的保守序列设计引物，利用RT-PCR技术结合3'-RACE扩增的方法，从新疆荒漠昆虫准噶尔小胸鳖甲Microdera punctipenis dzunarica中获得了长约294 bp不含信号肽的抗冻蛋白cDNA片段，命名为MpAFP5，其全长序列为363 bp(GenBank注册号为：AY821792)。

In this thesis, the integrated cDNA sequences of hira genes have been isolated from full-matured ovary SMART cDNA library of gynogenetic gibel carp and bisexual coloreded crucian carp by PCR and RACE-PCR.

本研究通过PCR和RACE的方法，分别从天然雌核发育银鲫和两性生殖彩鲫成熟卵细胞的SMART cDNA文库中克隆出hira全长基因。

Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen.

方法利用RT-PCR结合RACE技术克隆短穗鱼尾葵花粉中泛变应原profilin的全长基因，并进行序列分析。

Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen. The sequence of the insert was analyzed.

利用RT-PCR结合RACE技术克隆短穗鱼尾葵花粉中泛变应原profilin的全长基因，并进行序列分析。

We have cloned gene, designated as PvfadA, for C18:0 Δ9 desaturase catalytic activity from Pavlova viridis by RT-PCR, RNA ligase mediated RACE and Overlap-PCR strategy.

我们通过RT-PCR、RNA ligase mediated RACE and Overlap-PCR方法从海洋微藻绿色巴夫藻中克隆到一个命名为PvfadA的脂肪酸去饱和酶候选基因。

First of all, we introduce the 60GHz rat-race which is symmetric and experimental, and then implement 10GHz wideband mixer by using TSMC SiGe 0.35um BiCMOS process and GCTC 2.0um HBT process in circuit design. In TSMC SiGe 0.35um,we design micro mixer with Cherry Hooper technique and active load with single ended output.

本论文首先介绍60GHz实验性质的rat-race对称电路，接著在电路设计方面以TSMC SiGe 0.35μm BiCMOS以及GCT 2μm InGaP/GaAs HBT 制程来研制应用於10GHz 之宽频混频器，其中在TSMC SiGe制程包含了使用Cherry Hooper技术混频器以及电流镜主动负载单端输出的微混频器。

In GCT 2.0 μm InGaP/GaAs HBT, there are a 5.2GHz single-in single-out micromixer and a 5.2GHz single-in single-out micromixer with lumped element rat-race.

而采用GCT 2μm InGaP/GaAs HBT制程则包含了5.2GHz单端输出单端输入微混频器与5.2GHz整合集总元件Rat-race的单端输出单端输入微混频器电路。

In SiGe BiCMOS process, there are a 5.2GHz CMFB micromixer, a 5.7GHz up-conversion micromixer using LC current combiner, a 5.2GHz LC current combine up-converter with lumped element rat-race and a 5.2GHz image rejection double quadrature down-converter.

其中SiGe BiCMOS制程包含了5.2GHz共模回授微混频器电路、5.7GHz 使用LC电流合成器之升频微混频器、整合集总元件Rat-race的5.2GHz LC电流合成升频器与5.2GHz镜像消除双正交四相位降频器等电路。

In this study, homologous amplification and RACE technique were used to clone the encoding gene (SpTPS1) of trehalose-6-phosphate synthase from Selaginella pulvinata, which is an endemic exrophilous plant in China. The full-length cDNA is 3223 bp long, containing an open reading frame of 2790 bp.

本研究以我国特有旱生植物垫状巻柏为材料，采用同源扩增与RACE技术相结合的方法克隆了海藻糖-6-磷酸合成酶基因SpTPS1，cDNA全长3223 bp，包括一个2790 bp的开放阅读框。

A novel 1886bp full-length sesquiterpene synthase cDNA was cloned from a high-yield Artemisia annua strain 001 by RACE strategy.

用RACE方法从青蒿高产株系001中克隆了一个新的1886bp的全长倍半萜合酶cDNA。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。