查询词典 quantitative determination

Real-time fluorescence quantitative PCR technique has been widely applied, such as mRNA expression, detections of DNA copy number and determination of mononucleotide polymorphism, as well as in the clinical medicine including accurate quantitative examination of mycobacterium tuberculosis, Type B and Type C hepatitis, AIDS virus, gonococcus, and chlamydia trachomatis.

实时荧光定量PCR的应用范围非常广泛，包括mRNA表达的研究、DNA拷贝数的检测、单核苷酸多态性的测定等，已广泛应用于医学临床，它能对结核分枝杆菌、乙型、丙型肝炎、爱滋病病毒、淋球菌、沙眼衣原体等病原体进行准确的定量检测。

Department of Chemical Pharmacology, Capital University of Medical Science, Beijing 100054, ChinaAbstract: A reversedphase high performance liquid chromatographic method was developed for the simultaneous quantitative determination of caffeine, dapsone and chlorzoxazone. The operation was carried out on a C18 column (250 mmX4.6 mm i.d., 5.0 μm) with the mobile phase of acetonitrilephosphate buffer including 0.02 mol/L KH2PO4 and 0.02 mol/L (C2H53N, pH 6.5(25:75 in volume ratio).

用反相高效液相色谱同时测定血清中咖啡因、氨苯砜、氯唑沙宗探针药物的质量浓度，以乙腈磷酸盐缓冲体系(含 0.02 mol/L的磷酸二氢钾和0.02 mol/L的三乙胺，pH 6.5)(体积比为25：75)为流动相，以安替比林为内标，经C18柱(250 mmX4.6 mm i.d。， 5.0 μm)分离，紫外检测器检测，使3种探针药物得到较好的分离，并且在有效血药浓度范围内线性良好。

On the basis of referring the foreign and domestic literatures and systematically generalizing the former research results, firstly, from the adsorption gas, coal spontaneous combustion and the heat resolves, thesis analyzes the ignitable gas occurrence. Secondly, though the experiment station of coal spontaneous combustion, thesis obtains the critical temperature 60 ℃～ 100 ℃, splits temperature 100℃～ 150 ℃, the fission temperature 150℃～ 180 ℃ and the speed-up temperature 210℃～ 250 ℃ and ignitable gas release rules in the several temperatures sections according to the results. Finally according to fire resource and the catastrophe theory, ignitable gas explosion characteristic and the influence factors have been conducted of the qualitative investigation and the quantitative determination. The gas explosion control technologies are put forward. It has built the certain foundation for the effective prevention gas explosion accident occurrence.

本论文采用理论分析和实验模拟相结合的综合研究方法，首先从煤层吸附瓦斯、煤氧复合过程及煤高温热解三方面对采空区可燃性气体的产生进行分析；其次通过对大型煤自然发火实验测试台的应用，根据实验结果得到临界温度60℃～100℃、干裂温度100℃～150℃、裂变温度150℃～180℃和增速温度210℃～250℃等几个温度段的可燃性气体释放规律；最后根据爆炸引火源，结合瓦斯爆炸流变-突变理论，对可燃性气体爆炸特性及影响因素进行了定性研究和定量测定，提出瓦斯爆炸事故的控制对策，为有效预防瓦斯爆炸事故的发生打下了一定的基础。

We collected venous blood of 4 millilitre from all the children,and quantitative determination blood LPS, LBP, mCD14 by limulus reagent kinetic turbimetric assay, flow cytometry technique and ELISA method.

所有SIRS患儿及正常儿童各取静脉血4ml，分别利用鲎试剂动态比浊定量测定法、流式细胞分析技术、酶联免疫试验进行血液内毒素、mCD14、LBP的定量测定。

Besides its high sensitivity and specificity, the semi-quantitative determination by"naked-eye detection"is possible by this method.

该方法用于氟离子的检测不仅具有高的选择性及专一性，且可半定量&裸眼&检测。

Objective To establish a competitive RT-PCR method for quantitative determination of IFN-γ mRNA and IL-4 mRNA and explore the optimal inductive condition in vitro. Methods IFN-γ mRNA and IL-4 mRNA in PBMC induced by PMA and calcium ionophore of a healthy volunteer and in unstimulated PBMC of 11 HVs and 13 asthma patients were determined quantitatively with the established competitive RT-PCR method using self-prepared internal standard as competitive templets.

Subject words 】 Competitive templet； IFN-γ； IL-4； mRNA； RT-PCR 检测细胞因子最早使用生物学或免疫学测定法，这两类检测可反映某种细胞因子分泌、吸附、消耗和降解后的净含量〔1〕，但不能为细胞因子产生过程中基因转录、翻译及翻译后调控机制提供必要的信息，也不能反映细胞或组织内细胞因子基因表达水平。

OBJECTIVE:Introduce spectrophotometry method for quantitative determination of pethidine hydrochloride.

王锦目的：用紫外分光光度法测定盐酸哌替啶制剂的含量。

An HPLC method using an external standard was developed for quantitative determination of the pirimicarb residues in wheat seed .

采用甲醇超声波提取和液液分配技术净化提取物，反相高效液相色谱测定，建立了检测小麦籽粒中抗蚜威残留量的方法。

Objective To develop a rapid RP-HPLC method for quantitative determination of resveratrol in mouse serum and tissue homogenates.

目的 建立可快速测定小鼠血清及部分脏器匀浆中白藜芦醇浓度的RP-HPLC法。

Gas chromatographic analysis of 2-chloroethyl ethyl carbamate WANG Yue-feng, YANG Rui, SHE Yong-hong, ZHANG Hong-chao (Sanonda Zhengzhou Pesticide Co., Ltd., Zhengzhou 450009, China) Abstract: Separation and quantitative determination of 2-chloroethyl ethyl carbamate was conducted using gas chromatography with FID using an OV-101 packed column and biphenyl as the internal standard. Column temperature was 190℃, the ignition chamber was 230℃, and the test chamber was 230℃.

2-氯乙基氨基甲酸乙酯气相色谱分析王跃凤，杨睿，佘永红，张宏超(沙隆达郑州农药有限公司，郑州 450009)摘要：采用气相色谱法，柱填充物为3% OV-101涂渍在 Chromosorb W AW DMCS (180~250m)上，柱温：190℃，气化室：230℃，检测室：230℃；内标物为联苯，用内标法对2-氯乙基氨基甲酸乙酯有效成分进行分离和检验。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了