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Gas chromatography was employed to quantify the output of metabolization,and it was found that the yield of diacetyl for the mutant UV-3 was 18.7 fold to the wild-type 10293,up to 1.045g·L~(-1).Acetoin and ethanol yields were decreased by 48.4%and 71.4%,respectively.The yield of acetate was increased by 34.6%.

利用气相色谱测定代谢产物量,分析诱变前后菌株代谢途径中碳源的流向,结果表明突变株UV-3的丁二酮产量提高18.7倍,达到1.045g/L,乙偶姻产量降低48.4%,乙醇产量降低71.4%,乙酸产量提高34.6%,且遗传性质稳定。

Objective To quantify the burden of Borrelia burgdorferi in different representative tissues from the murine host by real time quantitative PCR.

目的 用实时荧光定量PCR确定伯氏疏螺旋体感染小鼠模型不同组织的病原体载量。

Label-free and cleavable isotope-coded affinity tag quantitative proteomic approaches were used to quantify the differential expression of secretory proteins from 3T3-L1 adipocytes with or without insulin stimulation.

首先,采用液相色谱-质谱联用方法对胰岛素刺激与否的3T3-L1脂肪细胞的分泌蛋白进行了大规模的定量分析;胰岛素刺激后,242种分泌蛋白表现为显著变化,其中55种蛋白质下调,187种上调。

Take DMSO swollen treatment and DEA-SO2-DMSO decrystallization treatment for example, the X-ray diffraction and the Tobolsky's intermittent stress relaxation of treated woods were determined during soaking in water, analyzed the effects of water on crystal degree and inter-cohesion of treated woods. According to these continuous relaxation curves measured in water with different temperatures, various thermodynamic quantities were obtained by using Eyring absolute rate theory, and reviewed the chemical reactions in wood which occur in different relaxation process. For the first time quantify these crosslinkings formed in the process of tensional relaxation by using the SMCIR intermittent stress relaxation way, and defined the cross-linking reaction types. In order to find out the contribute of drying to the fixation of deformation of chemically treated wood, stress relaxation of oven-dry untreated and treated wood was measured during the process of temperature elevation and descend, then analyzed the effect of temperature change on relaxation mechanism of treated oven-dry wood. According to continuous relaxation curves of oven-dry treated wood under various constant temperature, calculated the thermodynamics of relaxation process and discussed the mechanism of molecule change in wood, at the same time, also quantified these cross-linkings produced in wood by intermittent method and on the basis of which the model of molecular change during relaxation process of chemically treated was constructed.

以DMSO膨胀处理及DEA- SO2-DMSO非晶化塑化处理为例,测定了两种处理木材在水浸渍过程中的X射线衍射及Tobolsky间歇应力松弛,分析了水对处理木材结晶度及内部凝聚力的影响;通过未处理和两种处理木材在不同温度水中的连续应力松弛测定,应用Eyring的绝对速度反应理论计算并获得了松弛过程中的各热力学量,分析了在水中松弛过程中不同阶段木材内部发生的化学反应;并首次采用SMCIR连续·不连续双曲线应力松弛法定量了轴向拉伸应力松弛过程中木材内部产生的架桥量,明确了交联反应的类型;为了了解干燥对处理木材塑性变形固定的影响,测定了未处理和两种处理绝干木材在温度下降过程和上升过程中的应力松弛,分析了温度变化对处理绝干木材应力松弛的影响;根据多个温度水平下的连续应力松弛测定曲线,计算松弛过程的热力学量,考察了绝干木材在松弛过程中内部发生的分子变化机理,同时也用间歇法定量了木材内部新形成的架桥量,并在此基础上构筑处理木材在松弛过程中内部分子构造的变化模型。

Because of characteristics of quantify, synthesis, earthliness of VaR's measuring risk, VaR has been widely applied in many banks, financial institutions and supervision institutions, and now has been becoming the international standard. Therefore, introducing VaR to Chinese is practically very important.

VaR方法因其测量风险的定量性、综合性、通俗性等特点而被许多银行、金融机构和监管机构广泛应用,目前正在成为金融风险管理的国际标准,将它引入到我国的风险管理中有重大的现实意义。

In this paper, evaluation of public organijation performance management-model frame has been drawn to deschbe the related field, and expatiatory evaluation index system is put forward. Furthermore, analytic hierarchy process-based fuzzy comprehensive means is tried to quantify the system.

提出了公共组织运作模型研究框架,并通过对其各个层面的深入分析,构建了公共组织绩效管理评价指标体系;运用基于AHP 多层次模糊综合法,对公共组织绩效管理评价模型进行评测,对绩效管理关联因素进行量化。

Objective: To quantify the extension of air trapping on expiratory HRCT of lung and correlate with PFT results.

观察肺部疾病患者呼气相HRCT上空气潴留征的范围,对呼气相HRCT进行定量指标的测量和分析,并分析其与肺功能试验指标的关系。

A digital image -based technique was proposed to characterize and quantify the heterogeneity of rock, and it was implemented into RFPA code in order to simulate the complete failure process of rock under both the exter

本文提出了用数字图像处理技术表征岩石非均匀性的基本方法,并通过把该技术实施到岩石破裂过程分析程序RFPA中,来模拟非均匀岩石在外载荷和渗流耦合作用下发生破裂的整个过程。

OBJECTIVE: To quantify the stress distribution on the intervertebral disc, interarticular process and iliolumbar ligament of the lumbar vertebrae.

目的:观察轴向载荷下L5~S1上关节突与下关节突之间的峡部、椎间盘和髂腰韧带的应变和表面应力情况。

Ovine immatured oocytes, matured oocytes and embryos from 2-cell stage to blastocyst produced in vitro were recovered, and RT-PCR was used to semi-quantify mRNA expression. Proteins of Cx43 and Cx45 were detected by using a combination of immunocytochemistry and Laser Scanning Confocal Microscope.

收集绵羊未成熟和成熟卵母细胞以及体外受精早期胚胎各发育阶段的卵裂球细胞,通过RT-PCR半定量检测Cx43和Cx45基因的mRNA表达量;通过免疫组织化学方法结合激光扫描共聚焦显微镜检测Cx43和Cx45蛋白在绵羊体外受精的早期胚胎发育过程中的表达情况及表达区域。

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