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Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

The shilly-shally which exhibited in He Yan and Wang Bi"s thoughts when they probed into the problem of "Being and Non-being", provided the opportunity for the "exceeding the Confucian ethical code and trusting in the Nature" of Ruan Ji and Xi Kang. the extremely showed individuality in Ruan Ji and Wang Bi"s thoughts of explanation and the damage it caused, made Pei Wei and Guo Xiang put their concern focus in the more truly level of society in the situation of "Being and Non-being". And the "wandering around the range of Yi"s arrow and Self-transforming in the profound and obscure state" of Guo Xiang especially presented the dual concerns for "Being and Non-being" and "Confucian ethical code and nature.

何晏、王弼在诠释"有与无"的问题时表现出来的徘徊，为阮籍、嵇康的"越名教而任自然"埋下了理论上的契机，而表现在嵇、阮这里的主体个性的极度张扬以及对社会所造成的冲击，又使得裴頠、郭象在"有与无"的语境中，将关注的焦点放在了更为真实的社会层面之上，而郭象的"游于羿之彀中与独化于玄冥之境"则更是表现出了对"有与无"以及"自然与名教"的双重关注。

In the thesis we put forward description tools for hairs in terms of curved texel and curved volumetric texture. Also we give mathematical expressions for them and their applications for different hair styles.

我们针对头发提出了两种描述工具：弯曲纹元和弯曲体纹理，并给出了它们的数学表达式以及对不同发型的描述方法。

For example, provide the pension to the police martyr's family, carry out preferential policy on the policemen in western underdeveloped areas, directly transfer police equipments to the police sector in underdeveloped areas, put into practice the vocation system with salary for all the policemen, practice "5 small projects"(i.e. small canteen, small library, small gymnasium, small bathroom, and small washhouse) for the police stations at the grass-roots level, care about the work and life of the policemen. Thus, the cohesion of the policemen is strengthened and the policemen can devote themselves whole-heartedly to the tense work free of worries.

如对警察英烈家属给以抚恤，对西部不发达地区警察给予政策倾斜，直接划拨警用装备交付不发达地区警察机关使用，对全体警察实行带薪休假制度，对基层派出所实行"五小工程"（即小食堂、小图书馆、小健身房、小浴室、小洗衣房），对警察的工作和生活予以关心照顾，使警察无后顾之忧，从而进一步增强了警心凝聚力，使他们全身心地投入到紧张的工作中去。

I soon found a way to convince him that I would do him no harm, and taking him up by the Hand laugh'd at him, and pointed to the Kid which I had kill'd, beckoned to him to run and fetch it, which he did; and while he was wondering and looking to see how the Creature was kill'd, I loaded my Gun again, and by and by I saw a great Fowl like a Hawk sit upon a Tree within Shot; so to let Friday understand a little what I would do, I call'd him to me again, pointed at the Fowl which was indeed a Parrot, tho' I thought it had been a Hawk, I say pointing to the Parrot, and to my Gun, and to the Ground under the Parrot, to let him see I would make it fall, I made him understand that I would shoot and kill that Bird; accordingly I fir'd and bad him look, and immediately he saw the Parrot fall, he stood like one frighted again, notwithstanding all I had said to him; and I found he was the more amaz'd, because he did not see me put any Thing into the Gun; but thought that there must be some wonderful Fund of Death and Destruction in that Thing, able to kill Man, Beast, Bird, or any Thing near, or far off; and the Astonishment this created in him was such, as could not wear off for a long Time; and I believe, if I would have let him, he would have worshipp'd me and my Gun: As for the Gun it self, he would not so much as touch it for several Days after; but would speak to it, and talk to it, as if it had answer'd him, when he was by himself; which, as I afterwards learn'd of him, was to desire it not to kill him.

可怜的星期五上次曾看到我用枪打死了他的敌人，但当时他站在远处，弄不清是怎么回事，也想象不出我是怎样把他的敌人打死的。可这一次他看到我开枪，着实吃惊不少；他浑身颤抖，简直吓呆了，差一点瘫倒在地上。他既没有去看我开枪射击的那只小羊，也没有看到我已把小羊打死了，只顾扯开他自己的背心，在身上摸来摸去，看看自己有没有受伤。原来他以为我要杀死他。他跑到我跟前，扑通一声跪下来抱住我的双腿，嘴里叽哩咕噜说了不少话，我都不懂。但我不难明白他的意思，那就是求我不要杀他。我马上想出办法使他相信，我决不会伤害他。我一面用手把他从地上扶起来，一面哈哈大笑，并用手指着那打死的小羊，叫他跑过去把它带回来。他马上跑过去了。他在那里查看小山羊是怎样被打死的，并感到百思不得其解。这时我趁此机会重新把枪装上了子弹。不久，我看见一只大鸟，样子像一只苍鹰，正落在我射程内的一棵树上。为了让星期五稍稍明白我是怎样开枪的，就叫他来到我跟前。我用手指了指那只鸟--现在我看清了，其实那是一只鹦鹉，而我原先把它当作苍鹰了。我刚才说了，我用手指了指那只鹦鹉，又指了指自己的枪和鹦鹉身子底下的地方，意思是说，我要开枪把那只鸟打下来。

In building the simulation models for manufacturing missions, based on the combination with the multi-agent method and the GOOPN method, the compoundable method is presented, which makes the models more intelligent. Based on the result above, combined with the GOOPN models and the GERTS model, we put forward EPN-GERTS model through embedding Petri net into GERTS model, which can simplify the complexity in building models, and also improve the defects of GERTS model in describing resource conflicts and Petri net model in building models. In building the simulation models for development missions, the improved GERTS model is present to describe the development processes in QRMS. Through dynamically computing the redo times in GERTS, the improved model can reflect the operation character of development process. The simulation results are more precise when we use the models above.

针对构建快速研制系统的仿真对象问题，提出了基于多Agent的快速研制系统可靠性复合仿真建模方法，增强了仿真模型的智能性；在此基础上，针对制造任务，将Petri网嵌入到GERTS网中，提出了用于快速研制系统制造任务可靠性仿真的EPN-GERTS模型，简化了建模过程，同时也能考虑任务之间的资源冲突；针对研制任务，提出了用于快速研制系统研制任务可靠性仿真的改进GERTS模型，该模型可以描述设计迭代过程的动态运行特点，使仿真结果更加准确。

Many persons are married not for love but for various purposes, such as earning more money abroad, having a much happier life abroad than interiorly as fremdness is heaven; so they do their best to go abroad, and many foreigners become their instrument. I mean majority are with above purposes, and minority put on pure wedding dress because of real love, for welfare and future created by couple of one mind. In fact, the key problem is not the language, but the purpose of marriage, for live together forever or others.

很多人并不是为了爱情而结婚，那是有很多各种各样的目的的，有的人为了在国外多赚些钱，有的人以为国外就是天堂，能过上比国内幸福百倍的生活，于是想尽各种各样的方法和途径来国外，于是很多国外人成了她们的利用对象，当然在这里我指的是有那么一大部分是有上述的目的的，也有其中那一小部分是真正的跨国恋爱，为了追求未来，两个人同心协力创造属于自己的幸福而披上了洁白的婚纱。

Dye can bring a little color to life, most clothing is color with dyes, modern manufacture dyes can be costly, natural dyes from plan t and animal products have been used since ancient times, so this week, we describe by natural way to dye world, the advice comes from information written by Jenny Tim of the intermedial technology development group in Briton, this anti group is now called practical action, there are several messages to put dye onto material, the vent message for example, can be used to dye wool with onion skins, for this example, use 100 grams of natural wool, the wool must be clean, leave it overnight in water and liquid soap, then wash it with clean water that is a little warm, gently out the water, a solution called a moderant is used in the dying process, a moderant helps fix the dye to the material, traditionally moderants were found in nature, wood ash is one of example, but chemical moderants such as ironare popular today, iron is so would in many stores, it is often mixed with queen of daughter, a finding powder commonly used in cooking.

使用染料可以为生活带来更多的色彩。许多的布料都是通过染料来上色的。现代工业制造的染料通常都非常的贵，从古时起，人们就知道从植物或者动物产品中提取天然染料。这周，我们就来讨论一下如何用天然的方法来给羊毛着色。这一建议来自英国中级技术发展组的Jenny Dean的信息。这一古老贫穷的小组现在被称为实用性活动。把染料加到材料上有几种方法。其中之一是大桶法。举例来说，就是用洋葱皮来染羊毛。这一例子中，用100g的天然羊毛。羊毛必须清洗。用液体肥皂浸泡过夜。然后用温的干净的水冲洗它。轻柔地把多余的水分压挤出来。一种叫做媒染的溶剂也用于染洗过程。媒染是用来固定染料和材料的。传统的方法是，媒染是来天然的，比如木灰。但是像明矾这样的化学媒染在今天也是很流行的。明矾在许多商场都有出售。它通常和一种经常被用于烹调的粉状物-酒石混在一起。我们用多一点点的辛苦，来交换多一点点的幸福

In this paper, the 3-D finite element relocating mesh method is used to calculate the temperature field and the thermal creep stress field of spillway dam and mid level outlet dam in certain roller compacted concrete gravity dam, sensitivity analysis have been down with different parameters, such as placing temperature, the location of cooling pipe, cooling temperature, time for water supply, ascended-layer of concrete, intermissive time and different placing time, and the commendatory construction scheme have been put forward in such analysis; according by the test result on the two scheme of concrete aggregate combination (basalt as coarse aggregate and limestone as fine aggregate, basalt as coarse aggregate and basalt as fine aggregate), simulation analysis on temperature field and thermal creep stress field of the commendatory construction scheme have been down, and analysis have also been down on temperature field and thermal stress field of the two different aggregate combination, the result indicates that the spillway dam and mid level outlet dam which adopted these two aggregate combination can both satisfy the require of design crack resistance in concrete, furthermore limestone as fine is better than basalt as fine aggregate; the effect of summer water storage and winter water storage on the temperature field and thermal stress field have been analyzed, comparing with winter water storage, in summer water storage scheme the maximum tensile stress at abnormal concrete in upstream fill is decreased by 0.1～0.3MPa and there are no evidence change of the maximum tensile stress at other parts, which indicate that summer water storage is avail for the crack resistance of upstream fill; according to the simulation calculating result and comparing temperature simulation calculating results and temperature controlling measures of some similarity projects both completed and construction, synthesis appraise have been carried to the design temperature controlling measures of dam, and the temperature controlling measures of this project have been brought forward, all these have great reference value on the design of temperature controlling measures and the optimization of the construction scheme.

本文采用三维有限元浮动网格法仿真计算程序，对某碾压混凝土重力坝的溢流坝段和中孔坝段分别进行了三维有限元温度场和温度徐变应力仿真计算分析，对浇筑温度、冷却水管的布置方式、冷却水温、通水时间、浇筑升程和间歇时间、不同开始浇筑日期等参数进行了敏感性分析，在此基础上提出了推荐的施工方案；根据两种骨料方案(玄武岩粗骨料+灰岩细骨料方案；玄武岩粗骨料+玄武岩细骨料方案)的混凝土性能试验研究成果，对推荐的施工方案进行了温度场和温度徐变应力仿真计算，并对比分析了两种不同骨料情况下的温度场和温度应力，结果表明溢流坝段及中孔坝段采用上述两种骨料均能满足混凝土的抗裂设计要求，且灰岩细骨料优于玄武岩细骨料；计算分析了夏季蓄水和冬季蓄水对坝体温度场和温度应力的影响，夏季蓄水方案与冬季蓄水方案相比，坝体上游变态混凝土区域最大拉应力小0.1～0.3MPa，其他部位最大拉应力无明显变化，夏季蓄水对坝体上游面混凝土的抗裂有利；根据仿真计算成果并通过比较国内一些已建和在建的类似工程的温度仿真计算成果及采取的温控措施，对大坝的设计温控措施进行了综合评价，并提出了该工程的温控措施，对温控设计和优化施工方案具有重要参考价值。

The main works of the paper are listed as follows:On the aspect of theory analysis, this paper is based on the full acquaintance of conditions of leisure research home and abroad, generalized the corresponding concepts and theories of recreational sport, intensive discussed the corresponding contents about leisure, recreational sport and leisure education. Moreover, concerned that launching education for recreational sport in colleges and universities will accord with the requirement which higher education cultivate the all-round development human being, accord with the trait of epoch which social economy highspeed development. There exist close relationship between education for recreational sport and college P.E. reform. The direction of P.E. reform should be scientific, lifetime and leisurable in the future. Put the forward that construct an educational model of recreational sport in college, it will help college student foster their habit of lifetime sport and better leisure lifestyle.On the aspect of practice research, this paper analysis on the questionnaire called " The survey on the factors of constraints in recreational sport of college student", this investigation included four hundred students in five colleges and universities in Sichuan province.

其具体研究工作如下：在理论分析方面，本文在充分了解国内外休闲研究现状的基础上，归纳总结了运动休闲相关的概念和理论，对休闲、运动休闲和休闲教育的相关内容进行了深入探讨，而且进一步就运动休闲教育在高校中的开展做出了理论层面的分析，论述了高校中开展运动休闲的教育，符合高等教育培养全面发展的人的需求，符合当今社会经济快速发展的时代特征，认为运动休闲的教育与高校体育改革有着紧密的联系，今后体育改革应该朝科学化，终身化和休闲化的方向发展，提出在高校建立运动休闲俱乐部的教育模式，认为运动休闲的教育有利于培养大学生终身体育的习惯和良好的休闲生活方式。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐