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When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

For my part, I think that they had better metamorphose all such aspiring heroes of universal noveldom into man weather-cocks, as they used to put heroes among the constellations,and let them swing round there till they are rusty, and not come down at all to bother honest men with their pranks.

照我的看法，他们还不如把这些普遍的小说世界里往上爬的英雄人物一概变形为风信鸡人，好像他们时常把英雄放在星座之中一样，让那些风信鸡旋转不已，直到它们锈掉为止，却千万别让它们下地来胡闹，麻烦了好人们。

To use the well-developed inspection technology, two problems are solved. First, a crank-rocker mechanism is designed to put up and down the probe; and then an output interface equipment is built, which enables the operator observe the measurement results on time, by the communication between the pachometer and the output interface equipment.

考虑利用已成形的测厚技术，本文主要解决了两方面问题：机械上设计了曲柄摇杆机构实现了测厚探头的自动抬放；通过设计输出接口显示装置，利用它与测厚仪之间的通讯实现了测量数据的远距离传输，保证了操作者可实时观测到测量数据。

They put women inprison for long terms of imprisonment,for makinga nuisance of themselves—that was the expressionwhen they took petitions in their hands to the doorof the House of Commons; and they thought thatby sending them to prison,giving them a day's im－prisonment,would cause them to all settle down a-gain and there would be no further trouble.

他们把妇女投入监狱，处以长期徒刑，理由是她们招人厌恶——这是他们手持请求书走向下议院大门时说的原话；他们以为把她们送进监狱，哪怕只关一天，就足以使她们安静下来，就不会再有麻烦了。

Tell you one of the most simple method: buy a pack of "soda" is about a dollar, with the proportion of 5 percent, which means 5 saleratus release, water release of 100 copies, with cotton dipped in wiping in the affected area, more than a few good clean, and very effective, my son the other day is a good clean, but I began to use such a ratio less effective, but then I have to put some strong, clean, the cotton have white lesion of the visco-down, after just a few, you may wish to try it!

告诉你一个最简单的方法：买一包&小苏打&大概是一元钱，用5%的比例，也就是说小苏打放5份，水放100份，用棉签蘸着抹在患处，多抹几次就好了，非常有效，我儿子前些天就是这样抹好的，但我开始用这样的比例不太见效，后来我放得浓一些，抹的时候，棉签都把患处的白的粘下来了，几次以后就好了，你不妨试一试！

It is a short, sympathetic step from identifying with Scottishness to accepting the SNP's central tenet: that Scots are a disregarded minority within Britain (contrary to evidence that they are over-represented in positions of power, from the prime minister on down), who really ought to put that right by claiming independence.

从让他们认识自己是英格兰人，到让他们接受苏格兰国民党的中心原则，这只不过是引起共鸣的一小步：在英国国内，苏格兰是一个不为人所重视的少数群体（虽然自首相而下，苏格兰人占大多数），他们只有通过要求独立，才能使得情势有所扭转。

Through the guidance of professional, we knew to get Xian Jie area before evaporate, lest bilge hampers vapour permeates the skin; Want after clean face thin use currents and other characteristics of a river moist frost, lest heat up steam burnable skin; The hot water of 90 ℃ above is put in washbasin again after all these is finished, wrap up good hair, shut eye Fu body; Face and washbasin want apart 10 centimeters or so, if feel stivy, can look up with dry down towel gently blot is facial drip, rest moment changes hot water to continue again fuming or steaming-treating diseases with fumes as in moxibustion or with steam generated by boiling medicine herbs, relapse so 2-3 second, need about 15 minutes in all; After steam is fumed rinse in time with Wenshui, take a side with cold water, frost of nutrition of the Tu Xie after wiping.

经过专业人员的指导，我们知道了在蒸面前得先洁面，以免污垢妨碍蒸汽渗透皮肤；洁面后要薄施水性滋润霜，以免热汽灼伤皮肤；这一切完成后再在脸盆中放入90℃以上的热水，包扎好头发，闭目俯身；脸与脸盆要相距10厘米左右，如感到气闷，可抬头用干软毛巾轻轻吸干面部水珠，休息片刻再换热水继续熏蒸，如此反复2-3次，共需要大约15分钟；汽熏后及时用温水冲洗，以凉水拍面，擦干后涂些营养霜。

When we yield uncomprehendingly to environment, any spirit of revolt that we may have had dies down, and our responsibilities soon put an end to it.

当我们对于环境不加以了解便屈服于其中，则我们可能具有的任何反抗之心便逐渐熄灭了。不久，我们的种种责任更使它完全死绝。

Crossed some armour a few days to go to that guest home, that guest great kindness is xenial he, write down some armour is deliciously bean curd, be in cruelly oppress put bean curd in dish of a few flavour.

过了几天某甲到了那位客人家去，那位客人盛情款待他，记得某甲好吃豆腐，便在鱼肉几味菜中都放入了豆腐。

Torre partially threw up the white flag when he put Miguel Cairo in leftfield in the eighth inning (he also wanted to give Johnny Damon's sore calves a few innings off). The sight of Cairo fighting the sun and trying to run down Mark Teixeira's long drive to left-center in the eighth -- it didn't seem like a fair fight for the career infielder -- kind of summed up the afternoon. The ball fell for a double, one of three on the day for Teixeira (4-for-5, three RBIs, three runs).王建民加油站 D0 f' u7 a- N

当Torre在八局时派Miguel Cairo去守左外野时，已经算是某种程度的举白旗投降了〔他也想让Johnny Damon疼痛的小腿能少用个几局〕，Cairo在八局时试著挡住阳光去追Mark Teixeira打到中左外野之飞球的画面〔当时看起来，对这位当了一辈子内野手的Cairo来说，实在不是一个公平的对决…〕，也算是为今天下午做了个总结，那个球最后落地形成二垒安打，也是Teixeira在这场比赛中打出的三发二垒打的最后一发，他今天五打数、四安打、打回三分、得三分。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。