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The extract was further iaolated with LC and purified with preparative HPLC, and uridine, adenosine, and thymidine were obtained.

为了提高菌体中总核苷的提取率，比较了干法和湿法提取对RCEF260菌体中总核苷含量的差异。

A uricase gene was cloned and sequenced from Bacillus subtilis strain CCRC 14199. The cloned uricase gene contained an open reading frame of 1491 nucleotides encoding a protein of about 55 kDa. A uricase-MBP fusion protein of about 98 kDa was expressed and purified.

尿酸氧化酵素基因是来自枯草杆菌CCRC14199的重组DNA，其基因序列包含1491 核甘酸约55 kDa蛋白质，及表现在麦芽糖结合蛋白大约98 kDa之融合蛋白质，并呈现高尿酸氧化酵素的活性(9.1 U/mg)。

Methods: rabbit bone marrow mesenchymal stem cells were purified, and then cultured in inducing medium containing transforming growth factor-β1; mtt assay was employed to evaluate the proliferative activity of induced cells; immunohistochemical assay was used to detect the expression of type ⅱ collagen; induced cells were transplanted into the knee of rabbit, and x-ray photography was employed to observe the repair and formation of cartilage.

纯化兔骨髓间充质干细胞；用含转化生长因子β1的诱导液培养骨髓间充质干细胞；以mtt测定诱导细胞的增殖活性；免疫组化检测ⅱ型胶原蛋白的表达；诱导后的细胞移植于白兔膝关节内，x线摄片观察软骨修复形成情况。

Methods: BMSCs were harvested from the femurs, tibias and humerus bones of the rabbit, then separated, purified and proliferated.

采用体外细胞培养技术自兔股骨、胫骨及肱骨中分离BMSCs进行纯化、培养。

Methods BMSCs were harvested from the femurs, tibias and humems bones of the rabbit, then separated, purified and proliferated.

采用体外细胞培养技术自兔股骨、胫骨及肚骨中分离BMSCs进行纯化、培养。

Methods: MSCs were isolated from bilateral thighbones and tibias of Wistar rats, purified by adhesive-screening method, expanded in vitro culture and labeled with DAPI.

无菌条件下取出大鼠双侧股骨及胫骨，冲洗骨髓腔获得细胞，贴壁筛选法纯化MSCs并进行体外培养、扩增。

Methods The rat MSCs were isolated primarily from the femurs and tibias of the Wistar rats. MSCs were cultured, proliferated and purified by passage culture. After being induced by bFGF, cultuered MSCs were divided into experimental groups (GM1 group, GDNF group and GDNF+ GM1 group) and control group.

取雄性Wistar大鼠股骨和胫骨骨髓，进行MSCs的体外培养、传代扩增及纯化。bFGF预诱导24 h后，依据加入的神经营养因子不同分为单唾液酸四己糖神经节苷脂（GM1）组、胶质源性神经营养因子组和GDNF+GM1组，以及对照组。

Liu KewuAbstract: Sucrase was isolated and purified from small intestine of pig and its properties had also been examined.

对猪小肠蔗糖酶(Sucrase，E.C.3.2.1.26)在分离纯化的基础上，对其性质进行了初步研究。

A light spritz of our refreshing blend of essential oils and purified water can do wonders for your world.

清淡的让人精神焕发的精油，溶入纯水中，能让你的世界变得美妙。

Therefore there is a need to develop a method by which large and purified populations of spermatids can be isolated, not only for the immediate requirement but also for cryoproservation for subsequent treatment cycles.

因此，有必要建立一种比较简单的、可获取大量纯化精子细胞的方法，不但可满足立即使用所需，也可进行冷冻保存用于以后的治疗周期。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失