查询词典 purified

Whey proteins which hydrolysed by the primary purified proteinase could stronger inhibit the active of ACE.

将提取的蛋白酶初步纯化，得到纯度更高的能水解乳清抑制ACE活力的蛋白酶。

The hydrolysates from oat xylan using Xyn Ⅰ Xyn Ⅱ and their mixture for hydrolysis were analysed by HPLC. It was showed that Xyn Ⅰ hydrolyzed mainly the unsubstituted regions of oat xylan, and hydrolytes were high xylooligosaccharides; whereas Xyn Ⅱ exhibited greater catalytic versatility than Xyn Ⅰ and were able to attack substituted regions of the polysaccharide and showed greater activity to low xylooligosaccharides than Xyn Ⅰ to give xylobiose as the main hydrolysate. The purified xylanases were acidic enzymes.

以纯化酶组分及其混合物水解燕麦木聚糖，采用高效液相色谱分析相应的水解产物，结果表明，Xyn Ⅰ降解燕麦木聚糖时，主要降解作用发生在底物中没有取代基的区域，水解产物的聚合度较高；Xyn Ⅱ对底物具有更强的适应性，能降解底物中有取代基的区域，相对Xyn Ⅰ酶组分，Xyn Ⅱ酶组分对低聚合度的木聚糖的活性更高，木二糖为主要降解产物。

RESULTS AND CONCLUSION Hyodeoxycholic acid was purified by recrystallization and column chromatography.

结果与结论从猪去氧胆酸中分离出一种主要杂质，建立了纯化猪去氧胆酸的方法。

Objective To explore how to improve the yield arid quality of rat islets with different purified methods.

目的 探讨不同纯化方法对提高大鼠胰岛收获效果的作用。

Results The histological staining revealed that the viability and the purity of the purified islets were above 95% and 85% respectively.

结果 组织学染色显示纯化后胰岛的活力和纯度分别在95％和85％以上。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

For the purpose of constructing mammary gland specific expressional vector, we use the updream regulation sequence of bovine BLG to regulate lacS gene to express in mammary gland. Positive clone cells are selected and purified by the expression Neo gene and report gene EGFP. So the cells can be used as sources to create transgenic animals by nuclear transfer techniques.

本研究以牛β-乳球蛋白基因上游调控成分作为启动子，嗜热菌糖苷酶基因lacS为目的基因，构建了lacS基因乳腺特异表达载体，并通过脂质体介导法转染体外培养的牛胎儿成纤维细胞，借助新霉素抗性基因和增强型绿色荧光蛋白报告基因来筛选、纯化阳性克隆细胞，为利用核移植技术制备转基因动物提供细胞来源。

Each purified lemon-flavored fish oil capsule provided 450 mg of EPA, 100 mg of DHA and 50 mg of other omega-3 fatty acids.

每个纯化柠檬味鱼油软胶囊提供450毫克的EPA100毫克的DHA，50毫克，其他欧米加-3脂肪酸。

Xanthan Gum, which is mainly made from starch, is a high molecular weight polysaccharide produced through fermentation with Xanthomonas Camperstris under the conditions of special nutrient medium, pH, O2-supply and temperature, then purified,dried and milled into white-like or light-yellow free-flowing powder.

黄原胶是一种生物合成胶，呈类白或淡黄色粉末状，是以淀粉为主要原料，由微生物黄单孢杆菌在特定的培养基、 PH 值、通氧量及温度条件下经纯种发酵，再经提炼、干燥、研磨而制成的高分子多糖聚合物。

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清，将纯化的抗磷酸酪氨酸抗体作为第一抗体，羊抗兔Ig酶结合物作为第二抗体，建立双抗体夹心酶联免疫吸附测定法。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。