查询词典 purified

The recombinant proteins were purified with affinity columns by the 6 histidines tag or Glutathione-S-Transferase they were fused to.

并根据所表达融合蛋白的6个组氨酸或谷胱甘肽-S-转移酶标记进行亲和纯化。

It was purified by immobilized metal ion affinity chromatography under native conditions because there was a 6 histidines tag at its amino acid terminus.

在间接ELISA和免疫印迹试验中，重组的基质蛋白可与马传贫阳性血清样品发生反应，而与健康马血清无任何反应。

Coli. The protein was expressed in a soluble form and as a fusion protein. There was a 6 histidines tag at its amino acid terminus. It was purified by immobilized metal ion affinity chromatography under native conditions.

所表达的蛋白是一种可溶性的融合蛋白，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。

Our experiments confirmed that two known inhibitors DRB and A3 Inhibited the activity of purified recombinant human CK2 holoenzyme, and that quercetin, its derivatives and some tyrphostins exerted stronger inhibitory effect on CK2 than DRB and A3. These results provided important experimental basis and a simple screening method for the development of more effective inhibitors of CK2 and their clinical application in the future.

本结果实验证实纯化的重组人CK2全酶可受到已知抑制剂DRB和A3的抑制，但槲皮素及其衍生物和某些Tyrphostins抑制CK2的作用较DRB和A3更强，这对于开发更有效的CK2抑制剂，为今后应用于临床提供了重要的实验依据和一种较为简便的筛选方法。

METHODS Recombinant human protein kinase CK2 α and β subunits were cloned and expressed by genetic engineering, and purified to homogeneity. The two subunits were mixed at equal molar ratio and reconstituted CK2 holoenzyme, which exerted the maximum biological activity.

方法利用基因工程克隆，表达和纯化获得重组人蛋白激酶CK2 α和β亚基，在体外等摩尔数混合构成有最大生物活性的重组CK2全酶，在不同条件下测定CK2的活性。

METHODS: Recombinant human protein kinase CK2 α′ and β subunits were cloned, expressed and purified. The 2 subunits were mixed at equal molar ratio to reconstitute CK2 holoenzyme.

利用基因工程技术进行克隆、表达和纯化，获得重组人CK2的α′及β亚基，并在体外等摩尔混合构成CK2全酶。

AIM To study the direct effect of amiloride on recombinant human protein kinase CK2 holoenzyme and its kinetics. METHODS Recombinant human protein kinase CK2 α and β subunits were cloned,expressed and purified by gene engineering.

蛋白激酶CK2是一种真核细胞内普遍存在的信使非依赖性丝氨酸蛋白激酶，是由两个催化亚基和两个调节亚基组成的异源四聚体(α2 β2 、α′2 β2 或αα′β2 ) [1，2 ] 。

METHODS: Recombinant human protein kinase CK2 α′ and β subunits were cloned, expressed and purified. The 2 subunits were mixed at equal molar ratio to reconstitute CK2 holoenzyme.

办法：利用基因工程技术进行克隆、表达和纯化，获得重组人CK2的α′及β亚基，并在体外等摩尔混合构成CK2全酶。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

Recently many food derived fibrinolytic enzymes have been found in various traditional asian foods, such as Japanese Natto, Tofuyo, Korean Chungkook-Jang, soy sauce, edible honey mushroom, fermented shrimp paste, and Chinese douchi, and have been purified from these foods.

近年来在许多不同的亚洲传统酿造食品如日本纳豆、豆腐、泡菜、韩国大豆酱、食用磨菇酱、发酵虾酱和酱油等中均发现含有纤维蛋白溶解酶，并且从中分离纯化出这种酶。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失