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The IWFs were purified by (NH4)2SO4 precipitation fractionation, and then applied to Sephadex G-75 column. The column eluates were collected separately according to A280 nm and injected into the health wheat leaves to examine the eliciting activities by PAL, PO assays.

IWF经硫酸铵粗沉淀后，将蛋白沉淀用重蒸水重新溶解后进行凝胶过滤柱层析，以280nm紫外吸收检测、收集不同的蛋白峰，分别注射到健康的Thatcher叶片中，以苯丙氨酸解氨酶(phenylalanine ammonialyase，PAL)、过氧化物酶(peroxidase，PO)防卫反应作为评价诱导活性的指标，检测活性成分在各部分中的分布和诱导活性的强弱。

Sm and Nd are separated and purified by using HIBA as an eluent through a very small volume of cation-exchange resin

该方法选用AG50W×8阳离子交换树脂，并先后采用不同的淋洗剂进行分离提纯。

The combined 50% and 95% ethanolic elutes were concentrated to yield the purified effctive part.

总有效成分纯度为36.87%，说明采用D101树脂分离纯化虎杖有效部位是可行的。

Used the dynamic adsorption law to determine the resin adsorption purified in Liuhaoya the yellow ketones matter craft condition is: Uses the AB-8 adsorption resin,On the liquid volume is 100～150 mL approximately,The type fluid density is 1% approximately, elutes the fluid density is 70%,Speed of flow 1 mL/min.

采用动态吸附法确定了树脂吸附纯化柳蒿芽中黄酮类物质的工艺条件为：采用AB-8吸附树脂，上液量约为100～150 mL，样液浓度约为1%，洗脱液浓度为70%，流速1 mL/min。

After process purification DNA needs once more magnetism bead purification processing, the magnetism bead under the specific liquid environment with the DNA adsorption, is fixed under the magnetic field function one side the centrifuge tube, only has DNA and the magnetism bead unifies in the together part is retained, then the use specific elutes the fluid clean, finally uses double steams the water to dissolve DNA, by now the magnetism bead and DNA were separated from, DNA preserved in the liquid, this obtained has purified, the high grade skeleton DNA sample.

在处理洗净DNA 需要一次更多磁性小珠洗净处理之后，磁性小珠在具体液体环境之下以DNA 吸附，是固定的在磁场作用一个边之下离心机管，只有有DNA 并且磁性小珠成一体在部份一起被保留，那么用途具体洗脱流动性干净，最后使用双词根水溶化DNA，在现在以前磁性小珠并且DNA 被分离了从， DNA 被保存在液体，这被获得净化了，高级最基本的DNA 样品。

The present application also features methods of eluting the purified polypeptide as well as the incorporation of the methods within a purification train.

本申请的特征还在于洗脱被纯化多肽的方法及纯化系列中该方法的并入。

An ELISA(enzyme-linked immunosorbent assay.) was developed for detection of bovine brucellosis by using LPS as antigen purified by Heating - phenol recommended by OIE (office international des epizootics).

采用世界动物卫生组织推荐的热酚法提取的脂多糖作为抗原，建立了牛布氏杆菌病的ELISA方法，对45份血清进行检测，证明本ELISA方法灵敏性较高，经阻断试验和交叉试验，证明特异性较好。

In order to maintain the catolyst at a high level of activity the feedstock must be carefully purified.

为保持催化剂的高度灵活性，必须对进料精心加以净化。

Recombinant wild type PrP purified from E. coli was used to form amyloid fibrils.

将 E。 coli 中纯化得到的重组蛋白质进行淀粉样蛋白质纤维之培养。

The enzymes were furth er separated and purified into 12 components after DEAE-32 chromatography and p reparative electrophoresis.

经DEAE-纤维素离子交换和制备电泳进一步分离纯化，得到12个单一组分。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。