查询词典 purified

Therefore, the Lord appointed Jeremiah to be an assayer to examine their worth, but sadly, they were found impure, like the dross after the silver has been purified.

所以上帝差派耶利米作检验者测试他们，可是不幸地发现他们的不纯净，像银子经炼过，却含有渣滓一样。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

The activity of the purified enzyme was strongly inhibited by sodium azide and partially inhibited by cysteine and dithiothreitol.

叠氮钠可强烈抑制漆酶活性，半胱氨酸和二硫苏糖醇可部分抑制漆酶活性。

A character's Azoth, his internal furnace of purified Pyros, begins at 1, but Merit points may be spent to increase it.

灵丹角色的灵丹，是他内部纯净圣火的熔炉，开始为1，不过优势点可以用来提升它。

Wastewater containing titanium pigment was treated by microfiltration membrane, Nanometer titanium can be purified and concentrated by use of microfiltration. Operational conditions were optimized and the model was built. Finally, the amount of wash water was reduced. Based on the above research, the whole set equipment was designed, which can meet the needs in a titanium industry with a capability of 500 ton per year. Backflush and automatic control were used in the equipment, which has been steadily run two years, has been widely adopted in titanium industry. The technology has been widely adopted in titanium industry.In the recent years, whole set equipment of ceramic membrane was increasingly used in the recovery of desired production from fermentation broth in China. A new membrane process integrated with pretreatment of flocculation or coagulation was exploited to recover Inosine. ZrO2 ceramic membrane was used in the work, and the average permeation flux could reach 150L/m2.h under pH value of 3, transmembrane pressure 0.1MPa, CFV 3m/s, and temperature higher than 70C. The recovery ratio of inosine is higher than traditional recovery method, although inosine degraded under acidic and high temperature condition.

实验表明，ZrO_2膜处理肌苷发酵液的合适条件为pH=3、操作压力为0.1MPa、膜面流速3m／s、温度大于70度，此时可以获得平均渗透通量150L／m~2h，但由于酸性条件、高温下肌苷降解，导致肌苷收率有所降低，但仍高于传统肌苷提取方法得到的收率；为进一步提高肌苷得率，缩短提取工序，实验研究了絮凝剂预处理发酵液，解决碱性条件下由于蛋白析出导致膜污染加重的问题，研究表明采用CaCl_2絮凝并调节pH=11，可以获得平均渗透通量为180L／m~2h，肌苷得率大于95％；比较了三种肌苷提取工艺得到的产品质量，采用预处理与陶瓷南京工业大学博士学位论文膜分离结合的除杂工艺得到的粗昔质量与陶瓷膜分离、离子交换及活性炭吸附工艺生产的粗普质量相当，均优于传统的提取工艺；设计了每小时处理12一巧吨肌普发酵液的陶瓷膜成套装置，采用部分内循环的方式降低设备的能耗，简化了肌昔提取方式，提高了产品的收率和得率，由于减少离子交换和活性炭吸附，使得工厂废水排放量降低30一40%，降低酸、碱用量，有利于环境保护。

A water soluble polysaccharide was isolated and purified from the dry leaves of bamboo that grows in Hubei Province.

采用纸色谱和气相色谱法研究了从湖北恩施地区生长的箬竹叶中分离纯化的水溶性多糖的单糖组成。

We compared the effect of sevoflurane on ciliary beat frequency with that of halothane and isoflurane using purified andcultured rat tracheal epithelial cells.

我们用纯化培养的大鼠气管上皮细胞比较了七氟烷与氟烷和异氟烷对纤毛活动频率的影响。

In those days it was possible to be purified of the guilt of such a crime,and Bellerophon was in due course absolved by King Proems of neighboring Tiryns.

在当时，这样的罪行还有被免责的可能。邻国提拉斯的普洛特斯国王让他逃过了此劫。

The mind is to be purified through bhakti.

头脑要通过巴克提来净化。

In the argument between invention and discovery on patent of biotechnology .I ,firstly, take back look at the definition of invention and discovery in practice of patent of biotechnology in western countries . then ,analyse the possibility of putting the result of basic bioresearch into patent .Finally ,draw a conclusion :we should focus on the achievement of research on natural material and gene invention should be patented as long as the material and gene which have been separated and purified have real value .

关于生物技术专利中的发明与发现之争，本文首先回顾了西方发达国家在生物技术专利实践中对发明与发现的界定，接着分析了生物技术基础研究成果的可专利性，最后得出结论，我们应淡化发明与发现之分，着重于"三性"审查，即只要天然物质和基因被分离和纯化以后具有工业实用性，就应该给天然物质和基因发明等基础研究成果授予专利。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失