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By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Supposing you have purified the nadis by asana and pranayama, and you have also awakened the charkas by prananyama and a few asanas, there then remains the awakening of sushumna.

假设你已经通过坐法及调息净化了经脉，你也已经通过调息及一些体式唤醒了轮穴，这样就剩下中脉需要去唤醒了。

Based on above experiments, I prepared transgenic tobacco plants harboring the expression cassette of the GUS gene controlled by the Hsp70 gene promoter. In the mean time, I also expressed in bacteria and purified the proteins encoded by the P1 and NIa cistrons of PSbMV.

在此基础上，我们又培育了含Hsp70基因启动子指导下的GUS基因的表达盒的转基因烟草，并在细菌细胞中表达和提纯了PSbMV P1、NIa的蛋白质。

Methods: Myoblasts isolated from rat muscle samples were cultured and purified clonally in vitro. The 3rd passage cells were incubated with medium including basic fibroblast growth factor and dimethyl sulfoxid.

体外分离培养大鼠成肌细胞并克隆纯化，培养至第3代时加入含有碱性成纤维细胞生长因子和二甲基亚砜的培养液诱导分化，进行RT-PCR分析和免疫荧光细胞化学染色鉴定。

The amount is much lower than that of traditional coagulants, purified water quality.

用量远低于传统混凝剂，净化后的水质优良。

The purified vWF was immobilized onto a coverslip. Cell rolling was induced in a parallel-plate flow chamber and observed by phase-contrast video microscope.

纯化的vWF固着在盖玻片上，在平行板液流室中进行细胞滚动研究，用相差电视显微镜观察。

METHODS: Rat BMSCs were in vitro isolated, cultured and purified by the whole bone marrow method. At the fourth passage, BMSCs at a density of 4×10^8/L were incubated in 12-well plate with a coverslip.

全骨髓法体外分离培养、纯化大鼠骨髓间充质干细胞，传至第4代时，按4×10^8 L^(-1)密度接种于置有盖玻片的12孔板内，制备细胞爬片。

A recombinant pl asmid (designated as p8.0) was obtaining by ligating these three fragments. The p8.0 was subcloned into a plasmid (designated as p8.2) containing entire LTR of EIAV DLA. The complete nucleotide sequence of DLA strain of EIAV was determined by sequencing the p 8.2 . The purified p8.2 was used to transfect donkey leuko cyte cultures.

将此8.0kb EIAV全基因再亚克隆到含有一完整EIAV DLA株长末端重复序列的质粒中，获得一含有EIAV驴白细胞弱毒前病毒全基因的重组质粒，将其命名为p8.2，经核苷酸序列分析，证明p8.2含有EIAV前病毒的全基因。

Biomass gas power generation technology is a renewable energy technology which is using gasification technology change a variety of agricultural, forestry wastes (such as rice husk, wood chips, straw, dung, garbage, sewage) into combustible gas, through deducting decoking, dehydration, cooling, purification process, the purified gas as a gas engine fuel to drive generator and change the heat into electricity.

生物质气体发电机组技术是一种可再生能源利用技术，是将多种农、林业废弃物（如食用菌菇菇肥、谷壳、木屑、秸秆、畜粪、生活垃圾、污水）利用气化技术转化为生物质气，通过除尘、除焦、脱水、冷却等净化过程，作为气体发动机的生物质燃料燃烧作功，带动发电机发电，将生物质能转化为电能。

Purified sucralose-6-acetate which had been recrystallized was hydrolyzed in th system of sodium methoxide/methanol solution, and the mixture was quenched with H+ ion exchange resin, and cake was washed with methanol. The eluate was concentrated to remove methanol and methyl acetate. The aqueous solution was decoloured with active carbon. The filtrate solution was concentrated and crystallized above 38℃for sever hours, the product sucralose was collected.

重结晶的三氯蔗糖-6-乙酸酯于CH3ONa/CH3OH体系中醇解，用H+型阳离子交换树脂中和，蒸去甲醇、乙酸甲酯等，在水相中用活性炭脱色，水溶液经浓缩，在38℃以上结晶数小时，分离得三氯蔗糖。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。