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For the asymmetric reduction ofα-hydroxyacetophenone and ethyl 4-chloro acetoacetate,-1-phenyl-1,2-ethanediol and-ethyl 4-chloro-3-hydroxybutanoate were produced by the purified enzyme, with the 99% and 94.3% e.e. value respectively.

在纯酶水平验证了近平滑假丝酵母(Candida parapsilosis CCTCCM203011)对外消旋苯基乙二醇的拆分机理，整个去消旋过程是由-苯基乙二醇次级醇脱氢酶和羰基还原酶共同催化完成的。

Since 20 years before , the desulfurizer has become essential critical facility of the iron and steel plant Along with utilization of the desulphurization technology and use of the desulfurizer, processed haze temperature and haze ingredient have had the past compares change, moreover used the haze afterheat technology, and purified sprayed water.

二十多年来，脱硫塔成为钢铁厂必不可少的重要设施。随着脱硫技术的运用及脱硫塔的使用，使处理后的烟气温度和烟气成分与过去相比发生了变化，而且利用上了烟气的余热，并对烟气中的粉尘用水喷淋净化。

There are, however, no specific Wnt agonists and no method for in vivo delivery of purified Wnt proteins.

然而，没有特异的Wnt激动剂，也没有办法在体内释放纯化的Wnt蛋白。

After obtaining highly active purified, Arabidopsis thaliana AHAS, the crystallization of AHAS-monosulfuron had been tried.

通过X-射线衍射实验，经过对全部衍射图像的整合、分析，最终得到了分辨率为2.9〓的晶体衍射数据。

Alums occur naturally in various mineral s and can be prepared and purified by crystallization from their solutions.

明矾天然存在于各种矿物中，大致有酸涩味，无色无臭，以白色结晶粉末形式存在。

Alums occur naturally in various minerals and can be prepared and purified by crystallization from their solutions.

明矾天然存在于各种矿物中，大致有酸涩味，无色无臭，以白色结晶粉末形式存在。

Papain was modified using different anhydrides. The average ratio of modified-NH2 was tested by a trinitrobenzenesulfonic acid method. The native and modified papain samples were purified by dialysis and their structures were characterized by UV and IR.

用不同酸酐对木瓜蛋白酶进行化学修饰，以三硝基苯磺酸法测定修饰酶的平均氨基修饰度，对修饰前后的木瓜蛋白酶分别纯化并通过UV-vis和IR对其结构进行了表征。

Prokaryotic expressed protein products of candidate cDNAs were purified by SDS-PAGE and used as immunogens to prepare murine antisera.

5利用SDS-PAGE分离原核表达产物，并以纯化的表达产物为免疫原免疫小鼠，制备抗血清。

Cinnamomea, antrocamphin A was purified from previous ethanol extraction using bioactivity-guided fractionation. As results, the antrocamphin A could significantly inhibit NO and PGE2 autacoids production in LPS-induced RAW 264.7 macrophage cells. Meanwhile, the mRNA and protein expression levels of iNOS and COX-2 were inhibited by antrocamphin A in a dose-dependent manner. Antrocamphin A also reduced the translocation of NF-κB induced by LPS, which was associated with the prevention of the degradation of I-κB, and subsequently decreased p65/p50 proteins level in the nucleus. This is the first report demostrating the A.

接著并以活性为导向的分离策略，从樟芝子实体乙醇抽出物中分离出具抗发炎活性成分antrocamphin A，续利用LPS诱导小鼠巨噬细胞（RAW 264.7）产生发炎的模式，解析antrocamphin A之抗发炎机制，结果证实antrocamphin A确可有效抑制一氧化氮自由基和前列腺素的生成，透过蛋白质表现分析得知，antrocamphin A之抗发炎活性是经由抑制一氧化氮生成酵素和第二型环氧酵素的mRNA表现，进而抑制了一氧化氮生成酵素、第二型环氧酵素和核转录因子-κB等酵素之表现。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。