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This anion pump is composed of two subunits, the catalytic 63KDa ArsA, a hydrophilic protein, when purified, exhibits oxyanion-stimulated ATPase activity, the hydrophobic 45. 5KDa ArsB protein, located in the inner membrane of E. coli, is the putative anion translocating channel and also serves as the anchor for ArsA to attach on the membrane.

ArsATP酶由水溶性的63KDa的ArsA蛋白和膜整合的46KDa的ArsB蛋白组成，ArsA蛋白是催化亚基，具有亚砷酸盐和亚锑酸盐激活的ATP酶活性，ArsB蛋白则构成转运亚砷酸盐和亚锑酸盐的阴离子通道，同时作为ArsA蛋白在膜上的结合位点。

The results showed that plenty of the purified DPV virions were obtained after the cell lysate was condensed by 5-fold by ultrafiltration,and the concentrated DPV fluid was ultracentrifuged with 300g/L sucrose cushion method and 300-600g/kg sucrose density gradient centrifugation methods.

结果显示，用切向超滤法将病毒培养物浓缩为原体积的1/5后，再采用300g/L蔗糖垫底超速离心和300～600g/kg蔗糖密度梯度离心的方法进行纯化，可获得大量的纯化病毒粒子。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

According to the design task, a crude oil depot B nearby a combined station is being to be constructed. So that the purified commercial crude oils can be transported out of the depot B to the terminal station and a refinery locating at the opposite direction.

本设计将根据设计任务书，在B油田联合站附近拟建一座原油库，以便联合站净化油经其中转到相向的外输首站及炼油厂。

The pigment extraction was loaded into a silica gel column and purified twice with the mobile phase of hexane/ethyl acetate (1:2, v/v) and chloroform/acetone (12:1, v/v) separately.

实验证明，本方法可以得到含量较高的褐藻黄素溶液，为褐藻黄素的大规模生产提供了一定的实验依据。

At last, the effective approach was found that the 024-antibiotic can be purified by freez-dry, methyl alcohol soaking, acetone, chloroform, and methyl alcohol continuously washing, alumina and silica gel column chromatography.

粗提制品的理化性质研究表明，024放线菌所产抗生素为无色晶体，易溶于水，可溶于甲醇和乙醇，且在甲醇中的溶解度较大，不溶或难溶于丙酮、氯仿、正丁醇、乙酸乙酯与苯。

Based on the N-terminal amino acid sequence BLAST search results and the fact that its activity is strictly Ca^2＋ dependent, the purified protein might be structurally and functionally related to the annexin protein family.

结合其N-末端序列BLAST搜索结果及其活性的钙依赖性，推测该蛋白是与膜联蛋白Ⅱ相关的一类蛋白质

The Air Purified Water Machine aims to provide convenient, high quality and safe drinking water to people in the area short of water.

设计空气纯水机的目的是为缺水地区提供方便、高质量和安全的饮用水。

Usually, the pigment is extracted by acid aqueous or alcohol solution and purified by different kinds of resins.

该色素主要采用酸性水溶液或醇溶液直接浸提，利用不同类型的树脂进一步去除杂质，得到色素产品。

Treatment of purified wood pulp with 18% sodium hydroxide solution produces alkali cellulose.

用18%氢氧化钠溶液处理纯化木浆生产出碱性纤维素。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失