查询词典 purified

We have purified the fusion protein by use of amylose chromatography and the purity of fusion protein was 91%.

纯化后的融合蛋白可以被兔血清识别，证明纯化后的融合蛋白仍具有较好的反应原性。

METHODS: Adipose was obtained from regions surrounding epididymis, retroperitoneal space and nape after anesthesis by pentobarbital, and adipose-derived mesenchymal stem cells could be harvested through type Ⅰ collagenase digestion. ADSCs were incubated in vitro and purified by differential adhesion.

戊巴比妥耳缘静脉麻醉，依次无菌取出附睾附近脂肪垫、腹膜后脂肪、颈背部脂肪，I型胶原酶酶解法分离出脂肪源间充质干细胞（adipose-derived mesenchymal stem cells，ADSCs），进行体外传代后差速贴壁培养纯化。

The purified fusion protein (200 μg/mL) was used to generate the antiserum.

表达产物经GSH-琼脂糖树脂4B纯化，得到了约200微克每毫升的融合蛋白。

Consulting the application manual of Novagen company, recombinant CpRNase HIIa and CpRNase HIIb were expressed and purified.

参照 Novagen 公司提供的操作手册，表达并纯化了重组的 CpRNase HIIa 和 CpRNase HIIb。

The results are as follows: 37℃ is the most favorable temperature for induction expression of SUMO-hEGF in BL21, 5.0g/L the most best concentration of arabinose, and 4h the best time for induction expression. Meanwhile, the quantity of expression is around 20.2%. The conclusion that hEGF is the purified protein is verified by Western blotting. It is a good foundation for the development of hEGF genetic medicine.

结果表明： SUMO-hEGF在BL21中的最佳诱导表达温度为37℃、诱导剂阿拉伯糖的最佳浓度为5.0g/L、最佳诱导表达时间为4h，表达量约为20.2%；Western blot 分析证实，纯化后的蛋白是hEGF，为进一步开发hEGF基因工程药物奠定了基础。

Methods Highly purified HCV Core, NS3, NS4, NS5 proteins were immobilized on solid matrix by biochip arrayer, the protein chip was used to detect the corresponding antibodies in human serum by chemiluminescent immunoassays.

利用生物芯片技术和化学发光技术将高度纯化的抗原HCV Core、NS3、NS4、NS5以特定微阵列固定在固相载体上，用化学发光免疫法检测抗HCV Core、抗HCV NS3、抗HCV NS4和抗HCV NS5。

The electrolyte was purified by copper arsenite to effectively remove Sb and Bi impurities in the copper electrolyte .

为有效脱除铜电解液中的Sb、Bi等杂质，采用亚砷酸铜净化电解液。

The oxidation speed and rate of pyrite and arsenopyrite are highly improved after the seperated and purified vaccine was put into the 9K culture media to be trained.

分离纯化后的菌种在加入毒砂和黄铁矿的9K培养基中驯化后对毒砂和黄铁矿的氧化速度和氧化率都有较大幅度的提高。

In this study, we isolate, purify and characterize endo-β-glucanase by Ascomycete Xylaria regalis. The cellulose-containing medium is used to induce the production of the cellulose-degrading enzymes. When these enzymes are produced at maximum level, we harvest and filtrate the culture medium. The filtrate is purified by anion exchanger column and hydrophobic interaction column (phenyl-650M). The purification fold of endo-β- glucanase increases 4.3 times, the specific activity approaches 3.7 unit/mg, and the recovery is 27.6%, respectively.

为了分离且纯化此株菌产生在培养基中的endo-β-glucanase，先将此菌在cellulose液态培养基中大量培养，在此酵素达到最高峰时，利用滤纸过滤将菌丝及菌液分开，收集粗酵素液，再经过阴离子交换树脂及疏水性层析管柱(Phenyl-650M)等步骤予以纯化，所得到最终酵素纯度为 4.3倍，比活性为3.7 unit/mg，回收率为27.6%。

My feeling shows that my bad heart entre into the perfect comfort,which is similar with asphodel after summer and fall,repeatly immerge into the purified water,feel as if thinker is comfortable and passion.

我觉的，这时自己久蒙尘垢的心灵顿然浸入了一片慰藉之中，就像经历了夏秋后的水仙球，重新浸入那纯净的清水之中一样，心智舒展，活力洋溢。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失