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119G/L .②T lymphocytes were seperated from the purified speen lymphocytes by filtration through nylon fibre columns.

进一步将脾淋巴细胞过尼龙毛柱获取T淋巴细胞。

MethodsThe ethyl acetate fractions from MeOH extracts of the spermoderm of Xanthoceras sorbifolia were partitioned and purified by means of several separation methods.

方法采用多种分离技术对文冠果种皮甲醇提取物的醋酸乙酯部位分离纯化，根据其理化性质和光谱数据进行结构鉴定。

Objectv To study the chemical of Stachys geobombycis by priliminary test Methods some researches were made of the extracted mixture for Stachys geobombycis from acidic ethanol,purified water and methanol by using the law of test tube and round filter paper.

目的 通过预试验，初步了解地蚕的化学成分。

METHODS: Bone marrow was sterilely isolated. BMSCs were isolated and purified by the density gradient centrifugation.

无菌条件下抽取猪骨髓液，密度梯度离心法分离纯化骨髓间充质干细胞。

METHODS: Triceps brachii was sterilely collected from 3 rats, and muscle-derived stem cells were isolated in vitro, and purified by adherence method.

取3只大鼠，无菌条件下切取肱三头肌，体外分离培养肌源性干细胞，贴壁法纯化扩增。

METHODS: Mouse bilateral femur was sterilely isolated. BMSCs were harvested by the Percoll density gradient centrifugation, and purified and proliferated by the adherent method.

无菌分离小鼠双侧股骨，Percoll密度梯度离心法分离骨髓间充质干细胞，再用贴壁筛选法纯化扩增。

METHODS: Rat double lower limbs were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. BMSCs were isolated and purified by the whole bone marrow adherence method and density gradient centrifugation.

无菌条件下分离大鼠双下肢，低糖DMEM培养液冲出骨髓，分别运用全骨髓贴壁法和密度梯度离心法体外分离骨髓间充质干细胞，利用差速贴壁原理对细胞进行纯化扩增。

METHODS: Bilateral sciatic nerve was sterilely dissociated from rats. Schwann cells were prepared and purified by low-concentration enzyme fast digestion and differential adherence.

无菌剥离大鼠双侧坐骨神经，应用低浓度酶快速消化法、差速贴壁法分离培养纯化许旺细胞。

METHODS: Bilateral testis of Kunming male mice aged 5-7 days were sterilely collected. Spermatogonial stem cells were isolated and purified using adherence and noncontinuity Percoll density gradient centrifugation.

无菌收集5~7 d龄昆明雄性小鼠双侧睾丸，采用差速贴壁联合非连续性Percoll密度梯度离心法分离纯化精原干细胞。

Adding purified stylar S-RNase from Prunus mume cultivar 'Gessekai' to liquid medium, for cultivating self- and cross-pollen at temperatures lot, 25℃ and 35℃, respectively, then determining the rate of pollen germination and the length of pollen tube after different periods of cultivating time.

在液体培养基中添加从果梅'月世界'花柱中纯化的S-RNase，并分别于10℃、25℃和35℃条件下培养自花、异花花粉，测定不同培养时间后的花粉萌发率和花粉管生长长度；离体条件下进行果梅花柱S-RNase降解花粉管RNA反应后，用琼脂糖凝胶电泳检测不同温度条件下自花、异花花粉管RNA被降解的程度。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。