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In order to study species resource from ruminal fluid, one strain of methionine-degrading bacterium MB6-1 was isolated and purified. 16 S rRNA encoding gene (16 S rDN4) from MB6-1 was amplified by PCR, and its nucleotide sequence was determined.

为分析研究山羊瘤胃液中甲硫氨酸降解菌群的物种资源，对经分离纯化获得的一株甲硫氨酸降解菌MB6-1，采用PCR方法扩增其16 S rDNA基因，并测定其基因的核苷酸全序列。

In order to study species resource from ruminal fluid, one strain of methionine- degrading bacterium MB6-1 was isolated and purified. 16 S rRNA encoding gene (16 S rDNA) from MB6-1 was amplified by PCR, and its nucleotide sequence was determined.

摘 要：为研究山羊瘤胃液中甲硫氨酸降解菌群的物种资源，对经分离纯化获得的一株甲硫氨酸降解菌MB6-1，采用PCR方法扩增其16 S rDNA基因，并测定其基因的核苷酸全序列。

Other than that, try to fill your red slots with Runed Cardinal Ruby or cheaper versions if you can't afford it. Put Reckless Ametrine in yellow slots and Purified Dreadtone in blue slots if the socket bonus is worth it (use the formula above if unsure). In general, it's not advisable to gem for hit, so as soon as you've gotten some better equipment, you should try to get the hit from items, rather than gems.

如果插槽奖励值得，那么在黄槽中插上魯莽的紫黃晶(12法术伤害10急速)在蓝槽中插上純淨的懼石(12法术伤害10精神)通常情况下，不建议插命中宝石，一旦你获得了更好的装备，你应该尝试从装备本身中获得命中属性而不是从宝石上。

It has been found that evodiamine and rutaecarpine, two of the bioactive chemicals extracted and purified from Chinese herb Evodiae Fructus, possesses different effects.

吴茱萸碱和吴茱萸次碱是由常用的中药材吴茱萸所萃取出来具有生物活性的物质。

It was investigated how to increase the yield and purity of tannin, flavone and oligoprocyanidms from the root and stalk of sanguisorba, and the stability of polyphenols from sanguisorba was studied, too. The chemical constitutes of sanguisorba were analyzed, the conditions on extracting tannin, flavone and oligoprocyanidins from sanguisorba by general lixiviating process and ultrasonic wave were optimize. The flavone and oligoprocyanidins were separated from sanguisorba and purified. The stability of polyphenol from sanguisorba was studied and the chemical structure of the products was analyzed.

本论文以地榆根茎为原料，对如何提高其主要活性成分鞣质、黄酮类物质以及低聚原花青素得率和纯度以及地榆多酚的稳定性进行了探讨；主要分析了地榆中化学成分，常规浸提和超声波浸提对地榆根茎中鞣质、黄酮类以及低聚原花青素提取条件的优化，地榆根中黄酮类和低聚原花青素的分离纯化，地榆多酚稳定性能以及产品的初步化学结构分析。

The results from bioassay showed that both the capsules of PuGV－Ps and the partially purified synergistic factor enhanced infection of Pseudaletia separata nuclear polyhedrosis virus in the larvae of armyworm significantly.

生物测定的结果表明，粘虫颗粒体病毒PuGV－Ps及其增效因子对粘虫核型多角体病毒PsNPV均有明显的增效作用。

The purified protein induced as antigen was used to immunize the rabbit, and the antiserum of WMV CP was prepared whose titer measured by ELISA was 1:6400; western blot analysis indicated that the antiserum could serologically react with CP.

以诱导的蛋白为抗原免疫家兔，制备了WMV CP抗血清，ELISA法测其效价为1/6400，Western blot分析能与CP发生血清学反应。

The UMG polysilicon can be produced by heating the purified (NH4)2SiF6 above 500℃ in H2/Ar atmosphere. FTIR and XRD results show that amorphous polysilicon granules can be prepared after heat-treatment of (NH4)2SiF6 in a reducing atmosphere. The reduction of silicofluoride salts is a viable method for the production of UMG-Si.

将纯度较高的氟化矽铵盐在氢氩气氛中加热到500℃以上，可制造出冶金级的多晶矽，由XRD、SEM、FTIR与热分析之实验结果显示，在还原气氛中经热处理后所得之粉体为多晶矽，此种方法可以作为制造冶金矽的新方法，进而制备出太阳能产业级的多晶矽。

The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps , including Ni 2+ chelating affinity chromatography and gel filtration.

大肠杆菌表达的单链抗体包涵体，用8 mol/L尿素裂解，经过Ni离子螯合亲和层析和凝胶过滤两步纯化后，纯度达到97%以上，再通过透析复性除去尿素。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失