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In order to make full use of this resource,the Rana skin collagen proteins were extracted,purified and the properties were studied.

本文为了充分利用中国林蛙资源，对蛙皮胶原蛋白进行了提取纯化和性质研究。

Methods Compounds were isolated and purified by various column chromatographic techniques and their structures were elucidated on the basis of spectral analysis (IR, ESIMS, 1HNMR, 13CNMR). The inhibitory activity against the proliferation of HL60, REH and Raji cell lines was determined by using MTT assay.

用色谱方法分离栀子中化学成分，用波谱技术(IR，ESIMS，1HNMR，13CNMR)进行结构鉴定，用四唑盐比色法测定化合物对白血病细胞（HL60人早幼粒细胞白血病、REH人急性淋巴细胞白血病、 Raji淋巴瘤）增殖的影响。

Following attachment to the host immunized with purified antigens, midgut antigens, salivary gland antigens and reinfestation by I. sinensis, the midgut of tick revealed rather strikingly pathological changes, especially of the tick feeding on host immunized with 105KD purfied antigens the basal lamina became thinner, looser, and broken.

中华硬蜱叮咬纯化抗原、中肠抗原、唾液腺抗原免疫接种组和再次感染组宿主后，中肠可发生一系列明显的病理变化，尤以叮咬105KD纯化抗原组宿主后中肠病理变化最为严重。

The crude PPG was purified with neutralization-absorption process and the experimental conditions of crystallization, decolor , dehydration and removement of metal irons were investigated respectively.

应用中和-吸附的工艺方法对聚醚进行精制，并且分别考察了结晶、脱色、脱水、脱金属离子等重要实验条件，讨论了使用不同的吸附剂，对聚醚精制效果产生的影响。

With ultrasonic wave and lysozyme, the recombination N protein is split from bacteria. We dissolve it in guanidine hydrochloride, purify it with Ni〓 affinity chromatography column and renature it in vitro. By testing the antigen of the purified N protein, it shows that its activity is very high.

建立了用超声波和溶菌酶裂解菌体，用盐酸胍溶解，Ni〓亲合层析柱纯化及体外复性等方法，经检测证明纯化的VSV重组核蛋白抗原具有较高的活性。

Fe and Al in the system were removed by precipitation at pH≈5. Co2 was obtained by precipitation at pH=9.5~10, it was then purified by reprecipitation. Co3O4 was obtained by calcining Co2 at 500℃ for 2h, the purity of which was 99.3%. The recovery rate of Co was 97%. Li2CO3 was obtained by adding Na2CO3 to the mother liquor, the purity of which was 97.8%. The one-time recovery for Li was 73%. The mother liquor can be reused after being cooled to below 10℃ and Na2SO410H2O is recovered.

体系中的Fe、Al从杂质在pH≈5时沉淀除去；Co2在pH为9.5~10沉淀得到，经酸溶解后二次沉淀提纯，500℃煅烧2.0h得Co3O4，其纯度达到99.3%，钴的回收率达97%，母液中Li离子加Na2CO3处理，沉淀得Li2CO3，其纯度达97.8%，锂的一次回收率达73%，母液经冷却至10℃以下除去Na2SO410H2O后可循环使用。

Healthy kunming mice mate to produce embryo. sacifice prognant mice at 14dpc, make preparation of fetal liver and born marrow. then proceed 40%-70% percoll gradient seperation to obtain nucleated erythroid cells. resuspend the purified erythroid cells in DMEM medium without serum, adjust cell concentration to 5×10〓.

正常健康昆明小鼠交配，待胚胎发育至14天时，处死怀孕母鼠，取胎鼠肝脏，用另一组成年小鼠制备骨髓；然后应用40％-70％Percoll梯度分离法分离胎肝和骨髓中的有核红细胞，纯化后的细胞用无血清DMEM培养基悬浮，调整细胞浓度为5×〓／ml。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

The results of the study suggested that:Ricinine and allergen were separated and purified from CBM respectively, and were ident...

研究结果表明： 1 从蓖麻饼中分别分离提取得到蓖麻碱和变应原，经检测分析后，鉴定为纯品。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷