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The mutant △RSD-9 K141D was subcloned into the pGEX-6p-1 expression vector and the △RSD-9 protein was purified by GST affinity chromatography and digested by Prescission Protease to cut the GST domain. Next, to further verify the intracellular interaction of RSD-9/△RSD-9 and rtSH3p13 proteins, laser confocalization study and co-immunoprecipitation method were used.

随后，我们又运用蛋白质在细胞内的荧光共定位实验和免疫共沉淀实验，在细胞内相互作用水平上，证明了RSD-9蛋白/△RSD-9蛋白都与rtSH3p13蛋白在胞内相互作用。

The fusion protein was prepared and purified to feed larvas of Prodenia litura and Phanerotoma planifrons to evaluate its insecticidal effects.

对融合蛋白进行纯化，通过口途径饲喂瓜绢螟和斜纹夜蛾幼虫，以检测目的蛋白对两种虫的毒杀作用。

Results By using two-phase solvent systems composed of petroleum ether-ethylacetate-methanol-water (22:25:23:17) at the flow rate of 2.0 mL/min, with rotating speed of 800 r/min, and at temperature of 25℃, the yield of d1-tetrahydropalmatine and of protopine purified from the total alkaloids being 16.9% and 14.7%, with purity of 99.3% and 98.8% respectively can be reached.

结果 石油醚－乙酸乙酯－甲醇－水(22：25：23：17)为溶剂体系，流速为2.0 mL/min，仪器转速800 r/min，温度25℃，从延胡索总碱中可一步纯化得到原阿片碱和延胡索乙素，得率分别为16.9%和14.7%，纯度分别为99.3%和98.8%。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

In experiment 1, a 5 weeks folate depletion protocol was employed, using 13-week old Hyline pullets assigned to receive a semi-purified folate-deficient diet or a commercial corn-soybean meal based diet supplemented with 1 mg/kg pteroylglutamic acid.

试验一：以叶酸缺乏之半纯化饲粮餵饲十三周龄之新母鸡，为期五周，藉以了解新母鸡叶酸缺乏时，血液相及血液生化值的变化，以作为叶酸是否缺乏之指标。

If we've been forgiven of our sin and purified from all unrighteousness there is no need for a purgatorial state.

如果我们已经被原谅的仲和纯化一切不正也无须purgatorial一个国家。

Then Rb and Sr are separated and purified by using a mixed eluent of pyridinium and DCTA complex.

本实验室在此基础上建立了一种新的Rb-Sr，Sm-Nd化学分离方法，以用于地质样品的同位素测试。

For the analysis of the non-kinase activity and the relationship between kinase activity and non-kinase activity, the recombinant full length MLCK was expressed and purified quantifiably with the E.coli expression system. The ATP binding site site-directed mutants of MLCK which lost the kinase activity were acquired.

为了更好地研究MLCK分子的非激酶作用以及激酶与非激酶两者的关系，本实验利用大肠杆菌表达系统大量表达并纯化出完整的MLCK分子，并对其ATP结合位点进行定点突变，获得无激酶活性的MLCK突变体。

Rabic vaccine is one of the earlest and the most successful vaccine used for human. It's development went through several stages: animal brain tissue live vaccine, inactivated vaccine, prima cells culture inactiveted vaccine and cells cullture purified vaccine. Rabic vaccine plays a positive role in that prevent and control rabic epidemic.

人用狂犬病疫苗是世界上最早研制成功和使用的疫苗之一，经历了用动物脑组织制备的活疫苗、灭活疫苗、原代细胞培养灭活疫苗和细胞培养精制纯化疫苗等几个阶段，它为人类防治和控制狂犬病的传播起了积极的作用。

Polysaccharide was isolated and purified from Chinese medicinal herb Ramulus mori, and determination method for Ramulus mori polysaccharide was developed in this study. Carbon clearance test and dinitrylfluorobenzene-induced delayed type hypersensitivity test were used to evaluate effects of RMPS on immune responses of mice treated with dexamethasone.

采用水提醇沉法从中药材桑枝中分离纯化多糖，并建立桑枝多糖的含量测定方法；采用碳粒廓清法和二硝基氟苯诱导小鼠迟发型变态反应试验法，观察桑枝多糖对地塞米松所致免疫低下模型小鼠免疫功能的影响。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。