查询词典 purified

12 In a sacred and purified place after establishing a seat neither too high nor too low of kusa grass, deerskin or natural cloth; thereupon sitting firmly on that seat controlling the mind and activities of the senses making the mind one pointed; one in realization should meditate by the science of uniting the individual consciousness with the Ultimate Consciousness for purifying the mind.

在一个神圣纯净的地方，用秙挲草，鹿皮或者自然织物，做一个既不高也不矮的座垫，稳稳地坐在上面，控制心意活动和感官活动，使得意念专一。为了净化心灵，在修习中他应该通过修炼个体意识与根本意识的融合统一来进行冥想。

In chapter three, we expressed and purified leaderless DsbE , and characterized its structural and functional properties.

在论文的第三章中，我们表达、纯化了引导肽缺失的DsbE蛋白，并研究了它的结构和功能性质。

De Wit seeds with petrol ether,purified water and 95% ethanol was studied using test tube method.The chemical components of Leucaena leucocephala de Wit seeds were preliminarily demonstrated using precipitation reaction and color reaction of multiple indicators.

方法采用试管反应法，对银合欢种子的石油醚、水、95%乙醇提取物进行研究，通过多种指示剂和显色剂的沉淀反应或颜色反应，对银合欢种子可能含有的化学成分进行初步研究。

MethodsThe mixture was extracted from Leucaena leucocephala de Wit seeds with petrol ether,purified water and 95% ethanol was studied using test tube method.The chemical components of Leucaena leucocephala de Wit seeds were preliminarily demonstrated using precipitation reaction and color reaction of multiple indicators.

方法采用试管反应法，对银合欢种子的石油醚、水、95%乙醇提取物进行研究，通过多种指示剂和显色剂的沉淀反应或颜色反应，对银合欢种子可能含有的化学成分进行初步研究。

The fractions of Ligroine and chloroform part of the the EtOH extraction were isolated and purified by column chromatography on silica gel and Sephadex LH 20 and recrystallization. Their structures were studied by using UV, IR, H-NMR, 13C-NMR, and MS, techniques.

运用硅胶、Sephadex LH-20柱色谱等方法对芫花乙醇提取物的石油醚及氯仿萃取部分进行化学成分研究，并利用UV、IR、1HNMR、13CNMR、MS等光谱技术鉴定其化学结构。

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

The PCR technique was used to amplify rDNA-ITS-1 of Lutjanus fulviflamma , then the purified PCR productions were cloned into T-vector and sequenced by M13+/-primers.

以特异性引物扩增了金焰笛鲷的核糖体第一转录间隔区（ITS-1），扩增产物经克隆后测序，测得ITS1长度为566 bp。

Methods Activate groups A and C meningococcal polysaccharide with cyanogens bromide and prepare into MenAPS-ADH and MenCPS-ADH derivatives by reacting with 1, 6-adipic acid dihydrazine. Mix MenAPS-ADH and MenCPS-ADH derivatives with purified tetanus toxoid at a certain mass ratio, then add l-ethyl-3(3-dimethylaminopropyl)-carbodiimide to prepare bulks of MenAPS-TT and MenCPS-TT conjugates respectively. Mix the two bulks at a certain ratio, add stabilizer and lyophilize to prepare group A+C meningococcal polysaccharide conjugate vaccine.

A群和C群脑膜炎球菌多糖在溴化氰的活化下，以1，6己二酰肼作为连接子，与精制破伤风类毒素在碳二亚胺作用下结合，再按一定的比例将两者混合，制成A+C群脑膜炎球菌多糖结合疫苗原液，加保护剂冻干后，制成A+C群脑膜炎球菌多糖结合疫苗。

The manganic manganous oxide is prepared from the purified manganese sulfate solution using hydrogen peroxide as oxidant under alkali medium of ammonia solution.

研究了从软锰矿制备硫酸锰，在碱性溶液中，以过氧化氢为氧化剂，制备高比表面优质四氧化三锰的新工艺。

The colour of most detected bands became light in the five media with the order of mannosan, mannose, xylan, glucose and pectin.(4)The pectinase in the supernatant was purified by centrifuge , acetone precipitation and sephadex G-75 cloumngel filtration. The specific activity was increased 9.8-fold with an activity recovery of 32.98%.The molecular weight of pectinase was 42.2KD estimated by SDS-PAGE.

确立了以葡萄糖培养基6h的发酵液为提取果胶酶的最初取样点，发酵液经离心，丙酮沉淀，Sephadex G-75葡聚糖凝胶柱层析后获得了凝胶电泳均一的样品，比活力较原酶液提高了9.8倍，回收率达到了32.98%，用SDS-PAGE凝胶电泳测的纯化后的果胶酶分子量为42.2KD。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷