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Methods PI 1 anti idiotype monoclonal antibodies against pemphigus and purified PAb from the serum of a patient with active pemphigus were used to establish an ELISA system for detecting the PAb.

目前常用的PAb检测法为免疫荧光法和免疫印迹法等，都需要一定的实验条件，难以在临床推广使用。

C. Smith. 7 compounds were separated and purified from the Illicium wardii A. C. Smith fruits selected in Gaoli Gongshan in Nujiang area of Yunnan province, their chemical structures were confirmed by means of physical and chemical properties analysis and spectrum analysis.

方法］通过对来自于云南怒江高黎贡山八角果实进行分离纯化，得到了7个化合物，并采用理化性质分析和光谱分析等手段确定其化学结构。

The recombinant immunotoxin BDI-PE38 was expressed mainly in soluble form and can be purified by Ni(superscript 2+)-NTA agarose.

在基因工程的实践中，包含体的形成是人们利用大肠杆菌表达外源蛋白的一大难题。

An indirected ELISA was established with the purified recombinant protein, after the square matrix titrate determination best density is 2.43μg/mL, the blood serum best dilution ratio is 1:40,Compares with ten reagents boxes, proved we establish the ELISA method has the high specificity and the sensitivity.

以纯化的重组蛋白建立了初步的间接ELISA方法，经方阵滴定确定最佳包被浓度为2.43μg/mL，血清最佳的稀释比为1：40，与万泰试剂盒相比较，证明我们建立的ELISA方法有较高的特异性和敏感性。

Results:(1) The optimum conditions for protease induction were: 26℃, initial pH 6.0 and inoculative quantity of fungal hyphae=1g/100ml, using chitin as the inducer;(2) Pr1 was purified and its molecular weight was determined as 33kDa.

结果：(1)以几丁质为唯一碳氮源，在26℃，初始pH值6.0，1%的菌丝接种量为最佳诱导条件；(2)分离得到的类枯草杆菌蛋白酶，并测定其分子量约为33kDa。

The iodo compound 1 was isomerized by LIDA-promoted heavy halogen migration to 2,6-difluoro-4-iodopyridine (11; 81%) which was purified by crystallization thus removing unaltered starting material (1; 16%).

该碘化合物1是由异构利达，促进重型卤素迁移到2，6 -二氟- 4 - iodopyridine（11； 81％）是由结晶纯化不变，从而消除起始原料（1； 16％）。

Chromosome DNA was purified by isopycnic centrifugation in a CsCl-ethidium bromide.

氯化铯-溴化乙锭梯度离心法纯化DNA。

The Schizosaccharomyces pombe plasma membrane H(superscript +)-ATPases are obtained by differential centrifugation, acid treatment and isopycnic centrifugation in a discontinuous sucrose gradient to study the effects of Ca(superscript 2+), CaM and CaM inhibitors on activity of purified plasma membrane H(superscript +)-ATPase.

经差速离心，酸处理，蔗糖密度梯度离心制备粟酒裂殖酵母质膜H-ATP酶，以研究Ca(上标 2+)、CaM以及CaM拮抗剂对纯化的质膜H-ATP酶活性的影响。

Methods:The green peel of Juglans Mandshurica were extracted by 95% ethanol and then separated and purified by silica gel column chromatography and gel column chromatography.

核桃楸青果皮，用95%乙醇提取，然后用硅胶柱色谱、凝胶柱色谱进行分离纯化。

METHODS Polysaccharide of G.lucidum were extracted and purified.The content,molar ratio and protein content of polysaccharide were determined by HPLC and Kjeldahl method.

提取和纯化灵芝子实体、菌丝体和发酵液多糖，应用高效液相色谱法和凯氏定氮法对多糖的含量、单糖组成及糖蛋白质含量作了分析、比较。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷